Mental health in hospitals: A cross-sectional study of the representations of professionals in a French university hospital: Considering mental health is essential for the individual and society. Healthcare professionals are at risk of poor mental health. On the occasion of WHO World Mental Health Day on October 10th, 2021, a survey was conducted by the Occupational Health Service and the Direction of quality in Healthcare of the University Hospital of Lyon (Hospices civils de Lyon, HCL). The aim of the study was to collect the representations and beliefs of hospital staff on the/their mental health. Methods: A short and anonymous questionnaire was sent electronically to the 22,904 HCL professionals, with a link towards a short educational video. Results: Seven hundred and sixty-six responses were collected. Representations of mental health were generally positive among professionals, with a transactional vision including known determinants such as living and working conditions. The most chosen words related to mental health were ?equilibrium? and ?well-being?. The measures considered to be the most relevant by professionals to promote mental health were the establishment of rest ?bubble? times, times for discussions (colleagues and management), prevention programs, easier access to a ?psy? and to the occupational health service. Conclusion: This survey contributed to a better understanding of the representations of HCL professionals regarding mental health and to their awareness in this field. These results can be used to develop a mental health promotion plan in the workplace, adapted to hospital professionals.

Considering mental health is essential for the individual and society. Healthcare professionals are at risk of poor mental health. On the occasion of WHO World Mental Health Day on October 10th, 2021, a survey was conducted by the Occupational Health Service and the Direction of quality in Healthcare of the University Hospital of Lyon (Hospices civils de Lyon, HCL). The aim of the study was to collect the representations and beliefs of hospital staff on the/their mental health.La prise en considération de la santé mentale est essentielle pour l'individu et la société. Les professionnels de santé sont à risque d'une santé mentale dégradée. À l'occasion de la Journée mondiale de la santé mentale de l'OMS le 10 octobre 2021, une enquête a été conduite par le Service de médecine et santé au travail (SMST) et la Direction qualité usagers et santé populationnelle (DQUSP) des Hospices civiles de Lyon (HCL). L'objectif était de recueillir les représentations et croyances des personnels hospitaliers sur la/leur santé mentale. Méthode: Un questionnaire court et anonyme a été adressé par voie électronique aux 22 904 professionnels des HCL, accompagné d'une courte vidéo pédagogique. Résultats: Au total, 766 réponses ont été recueillies. Les représentations de la santé mentale étaient globalement positives parmi les professionnels, avec une vision transactionnelle incluant les déterminants connus comme les conditions de vie et de travail. Les mots les plus choisis en lien avec la santé mentale étaient « équilibre » et « bien-être ». Les mesures considérées comme les plus pertinentes pour promouvoir la santé mentale par les professionnels étaient la mise en place de temps de « bulle » de repos, des temps d'échanges (collègues et encadrement), des programmes de prévention, l'accès facilité à un(e) « psy » et au service de médecine et santé au travail. Conclusion: Cette enquête a contribué à mieux connaître les représentations des professionnels des HCL quant à la/leur santé mentale, et a participé à leur sensibilisation dans ce domaine. Ces résultats pourront participer à élaborer un plan de promotion de la santé mentale au travail adapté aux professionnels hospitaliers

Loss of phosphoinositide 3-kinase γ decreases migration and activation of phagocytes but not T cell activation in antigen-induced arthritis

<p>Abstract</p> <p>Background</p> <p>Phosphoinositide 3-kinase γ (PI3Kγ) has been depicted as a major regulator of inflammatory processes, including leukocyte activation and migration towards several chemokines. This study aims to explore the role of PI3Kγ in the murine model of antigen-induced arthritis (AIA).</p> <p>Methods</p> <p>Development of AIA was investigated in wildtype and PI3Kγ-deficient mice as well as in mice treated with a specific inhibitor of PI3Kγ (AS-605240) in comparison to untreated animals. Inflammatory reactions of leukocytes, including macrophage and T cell activation, and macrophage migration, were studied <it>in vivo </it>and <it>in vitro</it>.</p> <p>Results</p> <p>Genetic deletion or pharmacological inhibition of PI3Kγ induced a marked decrease of clinical symptoms in early AIA, together with a considerably diminished macrophage migration and activation (lower production of NO, IL-1β, IL-6). Also, macrophage and neutrophil infiltration into the knee joint were impaired <it>in vivo</it>. However, T cell functions, measured by cytokine production (TNFα, IFNγ, IL-2, IL-4, IL-5, IL-17) <it>in vitro </it>and DTH reaction <it>in vivo </it>were not altered, and accordingly, disease developed normally at later timepoints</p> <p>Conclusion</p> <p>PI3Kγ specifically affects phagocyte function in the AIA model but has no impact on T cell activation.</p

Defective dendritic cell migration and activation of adaptive immunity in PI3Kγ-deficient mice

Gene-targeted mice were used to evaluate the role of the gamma isoform of phosphoinositide 3-kinase (PI3Kγ) in dendritic cell (DC) migration and induction of specific T-cell-mediated immune responses. DC obtained from PI3Kγ−/− mice showed a reduced ability to respond to chemokines in vitro and ex vivo and to travel to draining lymph nodes under inflammatory conditions. PI3Kγ−/− mice had a selective defect in the number of skin Langerhans cells and in lymph node CD8α(−) DC. Furthermore, PI3Kγ−/− mice showed a defective capacity to mount contact hypersensitivity and delayed-type hypersensitivity reactions. This defect was directly related to the reduced ability of antigen-loaded DC to migrate from the periphery to draining lymph nodes. Thus, PI3Kγ plays a nonredundant role in DC trafficking and in the activation of specific immunity. Therefore, PI3Kγ may be considered a new target to control exaggerated immune reactions

Tripartite motif containing protein 27 negatively regulates CD4 T cells by ubiquitinating and inhibiting the class II PI3K-C2β

The K+ channel KCa3.1 is required for Ca2+ influx and the subsequent activation of CD4 T cells. The class II phosphatidylinositol 3 kinase C2β (PI3KC2β) is activated by the T-cell receptor (TCR) and is critical for KCa3.1 channel activation. Tripartite motif containing protein 27 (TRIM27) is a member of a large family of proteins that function as Really Interesting New Gene (RING) E3 ubiquitin ligases. We now show that TRIM27 functions as an E3 ligase and mediates lysine 48 polyubiquitination of PI3KC2β, leading to a decrease in PI3K enzyme activity. By inhibiting PI3KC2β, TRIM27 also functions to negatively regulate CD4 T cells by inhibiting KCa3.1 channel activity and TCR-stimulated Ca2+ influx and cytokine production in Jurkat, primary human CD4 T cells, and Th0, Th1, and Th2 CD4 T cells generated from TRIM27−/− mice. These findings provide a unique mechanism for regulating class II PI3Ks, and identify TRIM27 as a previously undescribed negative regulator of CD4 T cells

The Class II Phosphatidylinositol 3 kinase C2β Is Required for the Activation of the K+ Channel KCa3.1 and CD4 T-Cells

The Ca2+-activated K+ channel KCa3.1 is required for Ca2+ influx and the subsequent activation of T-cells. We previously showed that nucleoside diphosphate kinase beta (NDPK-B), a mammalian histidine kinase, directly phosphorylates and activates KCa3.1 and is required for the activation of human CD4 T lymphocytes. We now show that the class II phosphatidylinositol 3 kinase C2β (PI3K-C2β) is activated by the T-cell receptor (TCR) and functions upstream of NDPK-B to activate KCa3.1 channel activity. Decreased expression of PI3K-C2β by siRNA in human CD4 T-cells resulted in inhibition of KCa3.1 channel activity. The inhibition was due to decreased phosphatidylinositol 3-phosphate [PI(3)P] because dialyzing PI3K-C2β siRNA-treated T-cells with PI(3)P rescued KCa3.1 channel activity. Moreover, overexpression of PI3K-C2β in KCa3.1-transfected Jurkat T-cells led to increased TCR-stimulated activation of KCa3.1 and Ca2+ influx, whereas silencing of PI3K-C2β inhibited both responses. Using total internal reflection fluorescence microscopy and planar lipid bilayers, we found that PI3K-C2β colocalized with Zap70 and the TCR in peripheral microclusters in the immunological synapse. This is the first demonstration that a class II PI3K plays a critical role in T-cell activation