157 research outputs found
Variability in supply and cross-shelf transport of pink shrimp (Farfantepenaeus duorarum) postlarvae into western Florida Bay
The variability in the supply of pink shrimp (Farfantepenaeus duorarum) postlarvae and the transport mechanisms of planktonic stages were investigated with field data and simulations of transport. Postlarvae entering the nursery grounds of Florida Bay were collected for three consecutive years at channels that connect the Bay with the Gulf of Mexico, and in channels of the Middle Florida Keys that connect the southeastern margin of the Bay with the Atlantic Ocean. The influx of postlarvae in the Middle Florida Keys was low in magnitude and varied seasonally and among years. In contrast, the greater postlarval influx occurred at the northwestern border of the Bay, where there was a strong seasonal pattern with peaks in influx from July through September each year. Planktonic stages need to travel up to 150 km eastward between spawning grounds (northeast of Dry Tortugas) and nursery grounds (western Florida Bay) in about 30 days, the estimated time of planktonic development for this species. A Lagrangian trajectory model was developed to estimate the drift of planktonic stages across the SW Florida shelf. The model simulated the maximal distance traveled by planktonic stages under various assumptions of behavior. Simulation
results indicated that larvae traveling with the instantaneous current and exhibiting a diel behavior travel up to 65 km and 75% of the larvae travel only 30 km. However, the eastward distance traveled increased substantially when a larval response to tides was added to the behavioral variable (distance increased to 200 km and 85% of larvae traveled 150 km). The question is, when during larval development, and where on the shallow SW Florida shelf, does the tidal response become incorporated into the behavior of pink shrimp
Calcium EXAFS Establishes the Mn-Ca Cluster in the Oxygen-Evolving Complex of Photosystem II â€
High-resolution X-ray spectroscopy of rare events: a different look at local structure and chemistry
Impact of glucocorticoid receptor density on ligand-independent dimerization, cooperative ligand-binding and basal priming of transactivation: a cell culture model
Glucocorticoid receptor (GR) levels vary between tissues and individuals and are altered by physiological and pharmacological effectors. However, the effects and implications of differences in GR concentration have not been fully elucidated. Using three statistically different GR concentrations in transiently transfected COS-1 cells, we demonstrate, using co-immunoprecipitation (CoIP) and fluorescent resonance energy transfer (FRET), that high levels of wild type GR (wtGR), but not of dimerization deficient GR (GRdim), display ligand-independent dimerization. Whole-cell saturation ligand-binding experiments furthermore establish that positive cooperative ligand-binding, with a concomitant increased ligand-binding affinity, is facilitated by ligand-independent dimerization at high concentrations of wtGR, but not GRdim. The down-stream consequences of ligand-independent dimerization at high concentrations of wtGR, but not GRdim, are shown to include basal priming of the system as witnessed by ligand-independent transactivation of both a GRE-containing promoter-reporter and the endogenous glucocorticoid (GC)-responsive gene, GILZ, as well as ligand-independent loading of GR onto the GILZ promoter. Pursuant to the basal priming of the system, addition of ligand results in a significantly greater modulation of transactivation potency than would be expected solely from the increase in ligand-binding affinity. Thus ligand-independent dimerization of the GR at high concentrations primes the system, through ligand-independent DNA loading and transactivation, which together with positive cooperative ligand-binding increases the potency of GR agonists and shifts the bio-character of partial GR agonists. Clearly GR-levels are a major factor in determining the sensitivity to GCs and a critical factor regulating transcriptional programs
Spatial variability in Florida Bay particulate organic matter composition: combining flow cytometry with stable isotope analyses
Glucocorticoid Receptor–Promoter Interactions: Energetic Dissection Suggests a Framework for the Specificity of Steroid Receptor-Mediated Gene Regulation
The glucocorticoid receptor (GR) is a member of the steroid
receptor
family of ligand-activated transcription factors. A number of studies
have shown that steroid receptors regulate distinct but overlapping
sets of genes; however, the molecular basis for such specificity remains
unclear. Previous work from our laboratory has demonstrated that under
identical solution conditions, three other steroid receptors [the
progesterone receptor A isoform (PR-A), the progesterone receptor
B isoform (PR-B), and estrogen receptor α (ER-α)] differentially
partition their self-association and promoter binding energetics.
For example, PR-A and PR-B generate similar dimerization free energies
but differ significantly in their extents of intersite cooperativity.
Conversely, ER-α maintains an intersite cooperativity most comparable
to that of PR-A yet dimerizes with an affinity orders of magnitude
greater than that of either of the PR isoforms. We have speculated
that these differences serve to generate receptor-specific promoter
occupancies, and thus receptor-specific gene regulation. Noting that
GR regulates a unique subset of genes relative to the other receptors,
we hypothesized that the receptor should maintain a unique set of
interaction energetics. We rigorously determined the self-association
and promoter binding energetics of full-length, human GR under conditions
identical to those used in our earlier studies. We find that unlike
all other receptors, GR shows no evidence of reversible self-association.
Moreover, GR assembles with strong intersite cooperativity comparable
to that seen only for PR-B. Finally, simulations show that such partitioning
of interaction energetics allows for receptor-specific promoter occupancies,
even under conditions where multiple receptors are competing for binding
at identical sites
Recommended from our members
FIELD OBSERVATIONS ON SELECTIVE TIDAL-STREAM TRANSPORT FOR POSTLARVAL AND JUVENILE PINK SHRIMP IN FLORIDA BAY
Post larvae and juveniles of pink shrimp were collected in the summers of 2005 and 2006 at three stations in northwestern Florida Bay. the main nursery ground of this species in South Florida. Collections were made at one- or two-hour intervals during three full moon nights and two new moon nights at depth intervals in the water column. Results of the five collections were consistent with the assumption that postlarvae use a flood-tide transport (FTT) to advance into the estuary by ascending in the water column during the dark-flood tide and resting near the bottom during the ebb tide. Evidence of a FTT were higher numbers of postlarvae per hour collected during the flood tide vs. ebb tide and the large number of postlarvae collected with highest velocity flood tide currents. ANOVA indicated significant differences in the number of postlarvae collected between tidal stages and moon phases, but not among depths. Post larvae were more abundant during new moon than full moon. We also found different patterns of postlarval distribution between the new and full moon. During the new moon, a large peak of postlarvae occurred coincident with highest current speeds, whereas, with one exception, during the full moon postlarvae were more abundant in the second half of the flood period near the slack tide. In contrast, juveniles exhibited a behavior and migratory pattern opposite to that of postlarvae. ANOVA indicated significant differences between the number of juveniles captured between tidal stages and among depths, but not between moon phases. Juveniles were found almost exclusively near the surface on the ebb tide. Significantly larger juveniles were captured on the dark-ebb rather than on the dark-flood tide during both moon phases, suggesting that older juveniles were leaving the Bay on the ebb tide
- …