Direct Cardiac Reprogramming: Progress and Promise.

The human adult heart lacks a robust endogenous repair mechanism to fully restore cardiac function after insult; thus, the ability to regenerate and repair the injured myocardium remains a top priority in treating heart failure. The ability to efficiently generate a large number of functioning cardiomyocytes capable of functional integration within the injured heart has been difficult. However, the ability to directly convert fibroblasts into cardiomyocyte-like cells both in vitro and in vivo offers great promise in overcoming this problem. In this review, we describe the insights and progress that have been gained from the investigation of direct cardiac reprogramming. We focus on the use of key transcription factors and cardiogenic genes as well as on the use of other biological molecules such as small molecules, cytokines, noncoding RNAs, and epigenetic modifiers to improve the efficiency of cardiac reprogramming. Finally, we discuss the development of safer reprogramming approaches for future clinical application

The von Karman equations for plates with residual strain

We provide a derivation of the Foppl-von Karman equations for the shape of and stresses in an elastic plate with residual strains. These might arise from a range of causes: inhomogeneous growth, plastic deformation, swelling or shrinkage driven by solvent absorption. Our analysis gives rigorous bounds on the convergence of the three dimensional equations of elasticity to the low-dimensional description embodied in the plate-like description of laminae and thus justifies a recent formulation of the problem to the shape of growing leaves. It also formalizes a procedure that can be used to derive other low-dimensional descriptions of active materials.Comment: 26 page

Motorcycle Potential Problems in Jakarta

After the economic crisis in 1997, motorcycles become a popular transport mode in Indonesian cities.However this condition leads to large number of violation and accident rate. This study tried to discuss howthis problem started and what possible measure can be taken to solve the problems, mainly based on literaturereview. The traffic data used were compiled from the data provided by PT Pamintori Cipta. Interview surveywas conducted to find people perception of motorcycles issues. The conclusion suggests that there is a needof further comprehensive study to find out what condition that makes motorcyclists voluntary return to usepublic transport and social engineering and intensive training program should be implemented for youngergeneration to improve traffic condition in Jakarta

Unjamming of Granular Packings due to Local Perturbations: Stability and Decay of Displacements

We study the mechanical response generated by local deformations in jammed packings of rigid disks. Based on discrete element simulations we determine the critical force of the local perturbation that is needed to break the mechanical equilibrium and examine the generated displacement field. Displacements decay as a power law of the distance from the perturbation point. The decay exponent and the critical force exhibit nontrivial dependence on the friction: Both quantities are nonmonotonic and have a sharp maximum at the friction coefficient 0.1. We find that the mechanical response properties are closely related to the problem of force-indeterminacy where similar nonmonotonic behavior was observed previously. We establish direct connection between the critical force and the ensemble of static force networks.Comment: 4 pages, 4 figure

Endogenous Wnt signalling in human embryonic stem cells generates an equilibrium of distinct lineage-specified progenitors.

The pluripotent nature of human embryonic stem cells (hESCs) makes them convenient for deriving therapeutically relevant cells. Here we show using Wnt reporter hESC lines that the cells are heterogeneous with respect to endogenous Wnt signalling activity. Moreover, the level of Wnt signalling activity in individual cells correlates with differences in clonogenic potential and lineage-specific differentiation propensity. The addition of Wnt protein or, conversely, a small-molecule Wnt inhibitor (IWP2) reduces heterogeneity, allowing stable expansion of Wnt(high) or Wnt(low) hESC populations, respectively. On differentiation, the Wnt(high) hESCs predominantly form endodermal and cardiac cells, whereas the Wnt(low) hESCs generate primarily neuroectodermal cells. Thus, heterogeneity with respect to endogenous Wnt signalling underlies much of the inefficiency in directing hESCs towards specific cell types. The relatively uniform differentiation potential of the Wnt(high) and Wnt(low) hESCs leads to faster and more efficient derivation of targeted cell types from these populations

Extent of force indeterminacy in packings of frictional rigid disks

Static packings of frictional rigid particles are investigated by means of discrete element simulations. We explore the ensemble of allowed force realizations in the space of contact forces for a given packing structure. We estimate the extent of force indeterminacy with different methods. The indeterminacy exhibits a nonmonotonic dependence on the interparticle friction coefficient. We verify directly that larger force-indeterminacy is accompanied by a more robust behavior against local perturbations. We also investigate the local indeterminacy of individual contact forces. The probability distribution of local indeterminacy changes its shape depending on friction. We find that local indeterminacy tends to be larger on force chains for intermediate friction. This correlation disappears in the large friction limit.Comment: 5 pages, 6 figure