39 research outputs found
SEA-Mediated Ligation Is Accelerated at Mildly Acidic pH: Application to the Formation of Difficult Peptide Junctions
No full textInternational audienceThe bis(2-sulfanylethyl)amido (SEA) ligation has been introduced in 2010 as a novel chemoselective peptide bond forming reaction. SEA ligation is a useful reaction for protein total synthesis that is complementary to the native chemical ligation (NCL). In particular, SEA ligation proceeds efficiently in a wide range of pH, from neutral pH to pH 3-4. Thus, the pH can be chosen to optimize the solubility of the peptide segments or final product. It can be also chosen to facilitate the formation of difficult junctions, since the rate of SEA ligation increases significantly by decreasing the pH from 7.2 to 4.0. Here we describe a protocol for SEA ligation at pH 5.5 in the presence of 4-mercaptophenylacetic acid (MPAA) or at pH 4.0 in the presence of a newly developed diselenol catalyst. The protocols describe the formation of a valyl-cysteinyl peptide bond between two model peptides
Insight into the SEA amide thioester equilibrium. Application to the synthesis of thioesters at neutral pH
No full textInternational audienc
Vesical sphincter problems in multiple sclerosis: a first-line approach to assessment and treatment
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Pronostic interest of urodynamic test under acute S3 and interest of tibial posterior stimulation in the determining of efficacy of the neuromodulation S3 during detrusor overactivityPronostic interest of urodynamic test under acute S3 and interest of tibial posterior stimulation in the determining of efficacy of the neuromodulation S3 during detrusor overactivity
No full textCarbohydrate dependence during hard-intensity exercise in trained cyclists in the competitive season : importance of training status
No full textÉpidémie de cryptosporidiose d’origine hydrique dans les Alpes Maritimes–novembre 2019
No full textCopper-binding motifs Xxx-His or Xxx-Zzz-His (ATCUN) linked to an antimicrobial peptide: Cu-binding, antimicrobial activity and ROS production
No full textOne-pot chemical synthesis of small ubiquitin-like modifier protein–peptide conjugates using bis(2-sulfanylethyl)amido peptide latent thioester surrogates
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