Radiation effective dose assessment of [51Mn]- and [52Mn]-chloride

In order to establish the potential of [51/52Mn]Cl2 as safe PET brain tracers, the radiation effective dose (ED) of [51Mn]- and [52Mn]-chloride has been assessed by using biokinetic models in anthropomorphic phantoms. Results showed that [52Mn]-chloride releases one hundred thirty times more radiation dose (ED = 1.35 mSv/MBq) than [51Mn]-chloride (ED = 1.02E-02 mSv/MBq). Although the maximum positron energy of 52Mn allows a PET image resolution similar to that of 18F, activities below 15 MBq should be administered

Atividades de importação e exportação do Laboratório de Quarentena "Costa Lima" no período de 1991 a 2000.

Resumo: O Laboratório "Costa Lima", da Embrapa Meio Ambiente, realizou entre 1991 e 2000 170 introduções referentes a 42 espécies de organismos benéficos (6 fungos, 12 bactérias, 2 nematóides entomopatogênicos, 7 ácaros predadores, 13 insetos parasitóides e 2 insetos predadores) e de outros microrganismos. Este trabalho relata todas essas introduções e exportações oficiais de agentes de controle biológico e de outros organismos úteis.bitstream/item/131402/1/2001DC01.pdfNa publicação, ISSN incorreto 1516-4675

Avaliação do efeito do Baculovirus anticarsia sobre Podisus nigrispinus (DALLAS, 1851), predador da lagarta da soja Anticarsia gemmatalis (HUBNER, 1818).

Este trabalho tem como objetivo conhecer os efeitos da ingestão de lagartas da soja infectadas com B. anticarsia pelo predador Podisus nigrispinus, avaliando-se os parâmetros biológicos de cada inseto.bitstream/CNPMA/5854/1/baculovirus_anticarsia.pd

Pulmonary tuberculosis followed by sarcoidosis in an HIV-infected patient: a case report and a simplified diagnostic flowchart for diagnosis and treatment of sarcoidosis

The diagnosis of sarcoidosis in a patient living with HIV infection is an uncommon event and a challenge for clinicians. Clinical manifestations are variable and fluctuating depending to adherence to ARV therapy and to the level of CD4 count. We analyze here one chronic case in which sarcoidosis appeared clinically two years after pulmonary tuberculosis. The course of the disease was influenced and prolonged by frequent interruptions of antiretroviral therapy. Moreover the diagnosis and the decision to treat have been delayed by the need of exclusion of other pathologies, principally tuberculosis reactivation/reinfection, other mycobacterial diseases, hematologic malignancies. We propose a simplified flowchart for diagnosis and follow up of sarcoidosis, which may also be applied to patients with HIV infection. Diagnosis of latent tuberculosis infection (LTBI) may be difficult in these patients, because the immunological paradox of sarcoidosis. For this reason, following exclusion of active tuberculosis, we advise to submit all sarcoidosis patients to IPT (isoniazid preventive therapy), when immunosuppressive therapy is started

Implementation of two data bases on biosafety of biocontrol agents.

Since 1994, two data bases are being developed at the National Research Center for Monitoring and Environmental Impact Assessment - (CNPMA/EMBRAPA), under a project entitled "Risk Analysis and Environmental Impact of the Use of Biocontrol Agents". The first, "Who is Who on Risk Assessment of Biocontrol Agents" is an international data base and stores information on researchers, institutions and laboratories involved in such studies. It is presently available on the Internet for on-line search. Over 300 specialists from around the world are currently included in the data base. The second, "Risk Assessment of Biocontrol Agents", stores information collected from scientific papers about methodologies for risk assessment of the use of biocontrol agents. Over 100 papers have been processed and available for consultation at CNPMA/EMBRAPA's library. These data bases are being developed under a research project, conducted at CNPMA. The contribution of this work is to identify researchers and institutions that work with this issue and provide fast communication among them to faster exchange of experiences, and also to facilitate the selection of methodologies that could be used in different studies

Interleukin-1 receptor-associated kinase 4 (IRAK4) plays a dual role in myddosome formation and Toll-like receptor signaling

Toll-like receptors (TLRs) form part of the host innate immune system, in which they act as sensors of microbial and endogenous danger signals. Upon TLR activation, the intracellular Toll/interleukin-1 receptor domains of TLR dimers initiate oligomerization of a multiprotein signaling platform comprising myeloid differentiation primary response 88 (MyD88) and members of the interleukin-1 receptor-associated kinase (IRAK) family. Formation of this myddosome complex initiates signal transduction pathways, leading to the activation of transcription factors and the production of inflammatory cytokines. To date, little is known about the assembly and disassembly of the myddosome and about the mechanisms by which these complexes mediate multiple downstream signaling pathways. Here, we isolated myddosome complexes from whole-cell lysates of TLR-activated primary mouse macrophages and from IRAK reporter macrophages to examine the kinetics of myddosome assembly and disassembly. Using a selective inhibitor of IRAK4\u27s kinase activity, we found that whereas TLR cytokine responses were ablated, myddosome formation was stabilized in the absence of IRAK4\u27s kinase activity. Of note, IRAK4 inhibition had only a minimal effect on NF-kappaB and mitogen-activated protein kinase (MAPK) signaling. In summary, our results indicate that IRAK4 has a critical scaffold function in myddosome formation and that its kinase activity is dispensable for myddosome assembly and activation of the NF-kappaB and MAPK pathways but is essential for MyD88-dependent production of inflammatory cytokines. Our findings suggest that the scaffold function of IRAK4 may be an attractive target for treating inflammatory and autoimmune diseases