Estudio de la obesidad y del sobrepeso como factores de riesgo de la prevalencia y severidad del asma en niños de Valencia

[email protected]; [email protected]; [email protected]: La obesidad y el sobrepeso se han descrito como factores de riesgo asociados a la prevalencia y severidad del asma en niños y adolescentes. El objetivo del estudio ha sido el valorar el papel de la obesidad en el asma infantil. Ámbito de estudio y sujetos:Estudio realizado en niños y adolescentes entre 8 y 15 años, elegidos por un muestreo aleatorio tipo cluster entre los niños que estudiaban en 80 colegios, el cual representa el 30% de los colegios de la ciudad de Valencia. Material y métodos:El análisis de los datos se organizó en dos grupos, obesos (aquellos niños en un percentil superior al 85 del Índice de Masa Corporal (kg/m2), tomando como referencia la población española) y no obesos, cuando no cumplian esta condición. Se calcularon la prevalencia de los diferentes parámetros con un intervalo de confianza al 95%, y el riesgo relativo (RR) de los síntomas compatibles con asma entre niños obesos comparándolos con los no obesos. Resultados: No se obtuvo un riesgo relativo significativo para la obesidad con respecto al asma en aquellos niños por encima del percentil 85. Por otra parte, un incremento en el riesgo en relación con la severidad del asma se observó con la obesidad, principalmente en el percentil 85 (RR = 1,51 de sufrir entre 4-12 ataques de pitos y RR = 1,86 de sufrir más de 12 ataques en niños obesos frente a los no obesos) Conclusiónes: En este estudio, no identificamos un riesgo más alto de asma entre niños obesos frente a los no obesos, aunque encontramos que hubiera un riesgo más alto de severidad de síntomas asmáticos. En relación con la severidad del asma, observamos un riesgo más alto de ataques de pitos y sibilancias entre los niños obesos en los percentiles 85 y 95 del Indice de Masa Corporal.Background: Obesity and overweight have been described as factors associated with asthma. Our aim was to evaluate the role obesity plays on asthma in children. Scope and subjects: A study carried out on children and teenagers between 8 and 15 years of age, chosen for a cluster-type random sampling from children who studied in 80 schools, which represents 30% of the schools in the city of Valencia. Material and Methods: The analysed data was organized into two groups, obese (from the Body Mass Index (Kg/m2)), showing children with a percentile over 85% of the measuring reference for the Spanish population) and non obese, when they did not fulfil this condition. The prevalence of the different parameters studied was calculated by an Interval of Confidence of 95%. The risk was calculated (Relative Risk) from those symptoms compatible with asthma among obese children compared to non obese children. Results: No significant relative risk (RR) was seen for obesity with regards to asthma in those percentiles of obesity over 85. Otherwise, an increase in the relative risk (RR) regarding the severity of asthma was seen in relation to obesity, mainly in the 85th percentile (RR = 1.51 of suffering between 4-12 wheezing attacks and RR = 1.86 of suffering more than 12 attacks in obese children as opposed to non obese children). Conclusions: In this study, we did not identify a higher risk of asthma among obese children than among non obese children, although we did find there was a higher risk of severity of asthmatic symptoms. As far as the severity of the asthma is concerned, we saw a higher risk of wheezing and whistling attacks among obese children with the 85th and the 95th percentiles according to the Body Mass [email protected]; [email protected]; [email protected]

Efecto del gen BMP15 sobre la distribución del tipo de parto en ovejas Rasa Aragonesa

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Relationship between the effects of the BMP15 gene and the polygenic effects on prolificacy in the rasa Aragonesa sheep breed

Los efectos del gen BMP15, localizado en el cromosoma X, sobre la media y la variabilidad de la prolificidad fueron estimados en la población Rasa Aragonesa, donde se analizaron 918.956 partos de ovejas de distintos genotipos (heterocigotas para el alelo FecXR o ROA®; y no portadoras). En primer lugar, se utilizaron modelos umbral que incluían o no el efecto del gen para estimar el efecto del genotipo BMP15 y su contribución a la variabilidad genética de la prolificidad en esta población. También se utilizaron otros dos modelos para estimar las interacciones entre el genotipo y los poligenes, así como el efecto del genotipo sobre la variabilidad de la prolificidad. Todos los análisis se hicieron con el programa ASReml. El efecto del genotipo sobre la media fue de 0,32 corderos adicionales por parto cuando el alelo ROA® se encontraba presente. Debido a que durante muchos años la selección por prolificidad en esta población se ha llevado a cabo desconociendo la presencia de esta mutación, los animales selectos portadores tienen un valor genético poligénico residual más bajo que los no portadores. No se encontró interacción significativa entre el genotipo y los efectos poligénicos sobre el valor genético. Aunque la interacción entre el genotipo y el conjunto de umbrales fue significativa, la diferencia entre ambos genotipos en la varianza de la prolificidad sobre la escala observada a una prolificidad media dada es escasa y muy poco relevanteThe effects of the BMP15 gene, located on the X chromosome, on mean prolificacy and its variability, were estimated in the Rasa Aragonesa sheep population through the analysis of 918,956 lambing records from ewes of different genotypes (FecXR or ROA® heterozygous ewes; and non-carrier ewes). Threshold models including or not the gene effect were first run to determine the effect of the BMP15 genotype and its importance in the total genetic variability of prolificacy. Two other models were also run to estimate the interaction between the BMP15 genotype and the polygenic background, as well as the effect of the genotype on the variability prolificacy. All the models were run using the ASReml software. The effect of the presence of the ROA® allele of the BMP15 gene on the mean prolificacy was 0.32 extra lambs per lambing. Due to the selection on prolificacy performed during many years in this population ignoring the presence of this major gene, animals carrying the mutation were found to have lower remaining polygenic estimated breeding values than non-carrier animals, and there was no interaction between the BMP15 genotype and the polygenic background. Although the interaction between the genotype and the set of thresholds was significant, the resulting between-genotypes difference of variance of prolificacy on the observed scale, at a similar mean litter size, was very low and not relevantPublishe

J-PLUS: analysis of the intracluster light in the Coma cluster

The intracluster light (ICL) is a luminous component of galaxy clusters composed of stars that are gravitationally bound to the cluster potential but do not belong to the individual galaxies. Previous studies of the ICL have shown that its formation and evolution are intimately linked to the evolutionary stage of the cluster. Thus, the analysis of the ICL in the Coma cluster will give insights into the main processes driving the dynamics in this highly complex system. Using a recently developed technique, we measure the ICL fraction in Coma at several wavelengths, using the J-PLUS unique filter system. The combination of narrow- and broadband filters provides valuable information on the dynamical state of the cluster, the ICL stellar types, and the morphology of the diffuse light. We use the Chebyshev-Fourier Intracluster Light Estimator (CICLE) to disentangle the ICL from the light of the galaxies, and to robustly measure the ICL fraction in seven J-PLUS filters. We obtain the ICL fraction distribution of the Coma cluster at different optical wavelengths, which varies from $\sim 7\%-21\%$, showing the highest values in the narrowband filters J0395, J0410, and J0430. This ICL fraction excess is distinctive pattern recently observed in dynamically active clusters (mergers), indicating a higher amount of bluer stars in the ICL compared to the cluster galaxies. Both the high ICL fractions and the excess in the bluer filters are indicative of a merging state. The presence of younger/lower-metallicity stars the ICL suggests that the main mechanism of ICL formation for the Coma cluster is the stripping of the stars in the outskirts of infalling galaxies and, possibly, the disruption of dwarf galaxies during past/ongoing mergers.Comment: 10 pages, 3 figures, 1 table. Accepted for publication in A&

Human Coronavirus Virulence Motifs and Virulence

Trabajo presentado en el XIV International Nidovirus Symposium (Nido2017), celebrado en Kansas City, Missouri (Estados Unidos), del 4 al 9 de junio de 2017We have shown that SARS-CoV E protein is a virulence factor that includes at least two virulence motifs: its ion channel (IC) activity encoded within the transmembrane domain and a PDZ binding motif (PBM) located at its carboxy-terminus. We showed that E protein pathogenicity was caused by the activation of different host signaling pathways. One of them was the activation of inflammasome, a process mediated by the conductance of Ca++ byEprotein IC activity, leading to an increased expression of IL-1beta, TNF-alpha and IL-6 levels. Another signaling pathway implied the activation of a proinflammatory response mediated by NF-kB activation. This activation was a consequence of E protein-syntenin binding mediated by PBM-PDZ interactions. This binding caused an increase of p38MAPK phosphorylation promoting the induction of acute respiratory distress syndrome (ARDS), edema and death of mice infected with a mouse adapted SARS-CoV. The relevance of p38 MAPK activation after infection with the mouse adapted SARS-CoV was confirmed by the protection of mice in the presence of an inhibitor of p38 MAPK, but not in its absence. These results illustrated the identification of an efficient coronavirus (CoV) antiviral. The presence of a virulence factor such as the PBM motif in E protein allows the virus to interact with more than 400 cell proteins containing PDZ motifs, conferring the virus the potential to control a high number of cell-signaling pathways increasing its replication and virulence. In fact, we are analyzing the proteome of the viral PBM-cellular PDZ interactions using system biology approaches. Frequently, the ARDS caused by lung infection with mild respiratory viruses is resolved before it evolves to serious edema. In contrast, after SARS-CoV infection frequently this resolution does not take place. We have shown the binding of E protein to a main mediator of edema resolution, the Na+ /K+ ATPase, and proposed that this may be one of the procedures by which edema recovery is prevented after SARS-CoV infection, either by inhibition of Na+ /K+ ATPase activity or by relocating this enzyme to another subcellular compartment. Deadly human CoVs as SARS- and MERS-CoVs have at least two viral proteins with IC activity and PBM motifs. Studies on the relevance of E and 3a SARS-CoV proteins in replication and virulence, and the interdependence among them have shown that the presence in the virus of at least E or 3a proteins was needed for virus viability. In fact, we have shown that the complementation between E and 3a proteins is mediated by the PBM motifs located at the carboxy-terminus of these proteins. Our studies on the interaction of SARS-CoV and MERS-CoV with the host, and the engineering of reverse genetics systems for each of these viruses, led us to the development of genetically stable vaccine candidates that provided full-protection against the challenge with the homologous virulent virus using mice models

Safety and immunogenicity of a modified pox vector-based HIV/AIDS vaccine candidate expressing Env, Gag, Pol and Nef proteins of HIV-1 subtype B (MVA-B) in healthy HIV-1-uninfected volunteers: A phase I clinical trial (RISVAC02)

Clinical Trial, Phase ITo investigate the safety and immunogenicity of a modified vaccinia virus Ankara vector expressing HIV-1 antigens from clade B (MVA-B), a phase-I, doubled-blind placebo-controlled trial was performed.Peer reviewe

Novel insights into host-fungal pathogen interactions derived from live-cell imaging

Acknowledgments The authors acknowledge funding from the Wellcome Trust (080088, 086827, 075470 and 099215) including a Wellcome Trust Strategic Award for Medical Mycology and Fungal Immunology 097377 and FP7-2007–2013 grant agreement HEALTH-F2-2010-260338–ALLFUN to NARG.Peer reviewedPublisher PD

The ratio of horizontal to vertical displacement in solar oscillations estimated from combined SO/PHI and SDO/HMI observations

In order to make accurate inferences about the solar interior using helioseismology, it is essential to understand all the relevant physical effects on the observations. One effect to understand is the (complex-valued) ratio of the horizontal to vertical displacement of the p- and f-modes at the height at which they are observed. Unfortunately, it is impossible to measure this ratio directly from a single vantage point, and it has been difficult to disentangle observationally from other effects. In this paper we attempt to measure the ratio directly using 7.5 hours of simultaneous observations from the Polarimetric and Helioseismic Imager on board Solar Orbiter and the Helioseismic and Magnetic Imager on board the Solar Dynamics Observatory. While image geometry problems make it difficult to determine the exact ratio, it appears to agree well with that expected from adiabatic oscillations in a standard solar model. On the other hand it does not agree with a commonly used approximation, indicating that this approximation should not be used in helioseismic analyses. In addition, the ratio appears to be real-valued.Comment: Accepted for publication in Astronomy & Astrophysics. 8 pages, 8 figure

CagI Is an Essential Component of the Helicobacter pylori Cag Type IV Secretion System and Forms a Complex with CagL

Helicobacter pylori, the causative agent of type B gastritis, peptic ulcers, gastric adenocarcinoma and MALT lymphoma, uses the Cag type IV secretion system to induce a strong proinflammatory response in the gastric mucosa and to inject its effector protein CagA into gastric cells. CagA translocation results in altered host cell gene expression profiles and cytoskeletal rearrangements, and it is considered as a major bacterial virulence trait. Recently, it has been shown that binding of the type IV secretion apparatus to integrin receptors on target cells is a crucial step in the translocation process. Several bacterial proteins, including the Cag-specific components CagL and CagI, have been involved in this interaction. Here, we have examined the localization and interactions of CagI in the bacterial cell. Since the cagI gene overlaps and is co-transcribed with the cagL gene, the role of CagI for type IV secretion system function has been difficult to assess, and conflicting results have been reported regarding its involvement in the proinflammatory response. Using a marker-free gene deletion approach and genetic complementation, we show now that CagI is an essential component of the Cag type IV secretion apparatus for both CagA translocation and interleukin-8 induction. CagI is distributed over soluble and membrane-associated pools and seems to be partly surface-exposed. Deletion of several genes encoding essential Cag components has an impact on protein levels of CagI and CagL, suggesting that both proteins require partial assembly of the secretion apparatus. Finally, we show by co-immunoprecipitation that CagI and CagL interact with each other. Taken together, our results indicate that CagI and CagL form a functional complex which is formed at a late stage of secretion apparatus assembly