Switchable ErSc2N rotor within a C80 fullerene cage: An EPR and photoluminescence excitation study

Systems exhibiting both spin and orbital degrees of freedom, of which Er3+ is one, can offer mechanisms for manipulating and measuring spin states via optical excitations. Motivated by the possibility of observing photoluminescence and electron paramagnetic resonance from the same species located within a fullerene molecule, we initiated an EPR study of Er3+ in ErSc2N@C80. Two orientations of the ErSc2N rotor within the C80 fullerene are observed in EPR, consistent with earlier studies using photoluminescence excitation (PLE) spectroscopy. For some crystal field orientations, electron spin relaxation is driven by an Orbach process via the first excited electronic state of the 4I_15/2 multiplet. We observe a change in the relative populations of the two ErSc2N configurations upon the application of 532 nm illuminations, and are thus able to switch the majority cage symmetry. This photoisomerisation, observable by both EPR and PLE, is metastable, lasting many hours at 20 K.Comment: 4 pages, 4 figure

Unique geometry of sister kinetochores in human oocytes during meiosis I may explain maternal age-associated increases in chromosomal abnormalities

The first meiotic division in human oocytes is highly error-prone and contributes to the uniquely high incidence of aneuploidy observed in human pregnancies. A successful meiosis I (MI) division entails separation of homologous chromosome pairs and co-segregation of sister chromatids. For this to happen, sister kinetochores must form attachments to spindle kinetochore-fibres emanating from the same pole. In mouse and budding yeast, sister kinetochores remain closely associated with each other during MI, enabling them to act as a single unified structure. However, whether this arrangement also applies in human meiosis I oocytes was unclear. In this study, we perform high-resolution imaging of over 1900 kinetochores in human oocytes, to examine the geometry and architecture of the human meiotic kinetochore. We reveal that sister kinetochores in MI are not physically fused, and instead individual kinetochores within a pair are capable of forming independent attachments to spindle k-fibres. Notably, with increasing female age, the separation between kinetochores increases, suggesting a degradation of centromeric cohesion and/or changes in kinetochore architecture. Our data suggest that the differential arrangement of sister kinetochores and dual k-fibre attachments may explain the high proportion of unstable attachments that form in MI and thus indicate why human oocytes are prone to aneuploidy, particularly with increasing maternal age

Mutant huntingtin enhances activation of dendritic Kv4 K+ channels in striatal spiny projection neurons

Huntington\u27s disease (HD) is initially characterized by an inability to suppress unwanted movements, a deficit attributable to impaired synaptic activation of striatal indirect pathway spiny projection neurons (iSPNs). To better understand the mechanisms underlying this deficit, striatal neurons in ex vivo brain slices from mouse genetic models of HD were studied using electrophysiological, optical and biochemical approaches. Distal dendrites of iSPNs from symptomatic HD mice were hypoexcitable, a change that was attributable to increased association of dendritic Kv4 potassium channels with auxiliary KChIP subunits. This association was negatively modulated by TrkB receptor signaling. Dendritic excitability of HD iSPNs was rescued by knocking-down expression of Kv4 channels, by disrupting KChIP binding, by restoring TrkB receptor signaling or by lowering mutant-Htt (mHtt) levels with a zinc finger protein. Collectively, these studies demonstrate that mHtt induces reversible alterations in the dendritic excitability of iSPNs that could contribute to the motor symptoms of HD

Revealing the Nature of Algol Disks through Optical and UV Spectroscopy, Synthetic Spectra, and Tomography of TT Hydrae

We have developed a systematic procedure to study the disks in Algol-type binaries using spectroscopic analysis, synthetic spectra, and tomography. We analyzed 119 H-alpha spectra of TT Hya, an Algol-type eclipsing interacting binary, collected from 1985-2001. The new radial velocities enabled us to derive reliable orbital elements, including a small non-zero eccentricity, and to improve the accuracy of the absolute dimensions of the system. High resolution IUE spectra were also analyzed to study the formation of the ultraviolet lines and continuum. Synthetic spectra of the iron curtain using our new shellspec program enabled us to derive a characteristic disk temperature of 7000K. We have demonstrated that the UV emission lines seen during total primary eclipse cannot originate from the accretion disk, but most likely arise from a hotter disk-stream interaction region. The synthetic spectra of the stars, disk, and stream allowed us to derive a lower limit to the mass transfer rate of 2e-10 solar masses per year. Doppler tomography of the observed H-alpha profiles revealed a distinct accretion disk. The difference spectra produced by subtracting the synthetic spectra of the stars resulted in an image of the disk, which virtually disappeared once the composite synthetic spectra of the stars and disk were used to calculate the difference spectra. An intensity enhancement of the resulting tomogram revealed images of the gas stream and an emission arc. We successfully modeled the gas stream using shellspec and associated the emission arc with an asymmetry in the accretion disk.Comment: 46 pages, 15 figures, 6 tables, accepted by Ap

Analysis of Resilience and its Relationships with Psychological Factors during Successful Aging

The objective of this research was to verify the relationship between resilience, successful aging, wisdom, quality of life, basic psychological needs perception, cognition and depression. The methodological design was quantitative and transversal. 161 elderly people of both sexes living in the city of Ivoti / Brazil participated. The instruments used were the Resilience Scale, Wisdom Scale, Geriatric Depression Scale, and Inventory of Basic Psychological Needs Perceptions for the Elderly, MMSE, and Whoqol-Old. Spearman's correlation analysis identified the relationship between resilience and quality of life variables, basic psychological needs and depression. In the linear regression analysis, we found a model in which the increase of resilience is associated to the personal competence, autonomy, wisdom, cognitive performance and past, present and future activities. We conclude that the resilience association with psychological aspects in the onset of old age is an important protective factor for the maintenance of successful aging

Enzimas degradadoras de parede celular e parasitismo de escleródios no biocontrole de mofo-branco em campo por Trichoderma spp.

Este trabalho teve como objetivo identificar os principais mecanismos utilizados por Trichoderma spp. para o controle biológico de Sclerotinia sclerotiorum, causador do mofo branco do feijoeiro comum

Nitric oxide precursors and congenital heart surgery: A randomized controlled trial of oral citrulline

ObjectiveThe study sought to determine whether citrulline supplementation, a precursor to nitric oxide synthesis, is safe and efficacious in increasing plasma citrulline concentrations and decreasing the risk of postoperative pulmonary hypertension.Study DesignForty children, undergoing cardiopulmonary bypass and at risk for pulmonary hypertension, were randomized to receive 5 perioperative doses (1.9 g/m2 per dose) of either oral citrulline or placebo. Plasma citrulline and arginine concentrations were measured at 5 time points. Measurements of systemic blood pressure and presence of pulmonary hypertension were collected.ResultsMedian citrulline concentrations were significantly higher in the citrulline group versus the placebo group immediately postoperatively (36 μmol/L vs 26 μmol/L, P = .012) and at 12 hours postoperatively (37 μmol/L vs 20 μmol/L, P = .015). Mean plasma arginine concentrations were significantly higher in the citrulline group versus the placebo group by 12 hours postoperatively (36 μmol/L vs 23 μmol/L, P = .037). Mean systemic blood pressure did not differ between groups (P = .53). Postoperative pulmonary hypertension developed in 9 patients, 6 of 20 (30%) in the placebo group and 3 of 20 (15%) in the citrulline group (P = .451), all of whom had plasma citrulline concentrations less than age-specific norms. Postoperative pulmonary hypertension did not develop in patients who demonstrated plasma citrulline concentrations in excess of 37 μmol/L (P = .036).ConclusionsOral citrulline supplementation safely increased plasma citrulline and arginine concentrations compared with placebo after cardiopulmonary bypass. Postoperative pulmonary hypertension did not occur in children with naturally elevated citrulline levels or elevations through supplementation. Oral citrulline supplementation may be effective in reducing postoperative pulmonary hypertension

A Way to Reopen the Window for Electroweak Baryogenesis

We reanalyse the sphaleron bound of electroweak baryogenesis when allowing deviations to the Friedmann equation. These modifications are well motivated in the context of brane cosmology where they appear without being in conflict with major experimental constraints on four-dimensional gravity. While suppressed at the time of nucleosynthesis, these corrections can dominate at the time of the electroweak phase transition and in certain cases provide the amount of expansion needed to freeze out the baryon asymmetry without requiring a strongly first order phase transition. The sphaleron bound is substantially weakened and can even disappear so that the constraints on the higgs and stop masses do not apply anymore. Such modification of cosmology at early times therefore reopens the parameter space allowing electroweak baryogenesis which had been reduced substantially given the new bound on the higgs mass imposed by LEP. In contrast with previous attempts to turn around the sphaleron bound using alternative cosmologies, we are still considering that the electroweak phase transition takes place in a radiation dominated universe. The universe is expanding fast because of the modification of the Friedmann equation itself without the need for a scalar field and therefore evading the problem of the decay of this scalar field after the completion of the phase transition and the risk that its release of entropy dilutes the baryon asymmetry produced at the transition.Comment: 19 pages, 3 figures; v2: minor changes, remark added at end of section 5 and in caption of figure 1; v3: references added, version to be publishe