79 research outputs found

    Optimal General Matchings

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    Given a graph G=(V,E)G=(V,E) and for each vertex v∈Vv \in V a subset B(v)B(v) of the set {0,1,…,dG(v)}\{0,1,\ldots, d_G(v)\}, where dG(v)d_G(v) denotes the degree of vertex vv in the graph GG, a BB-factor of GG is any set F⊆EF \subseteq E such that dF(v)∈B(v)d_F(v) \in B(v) for each vertex vv, where dF(v)d_F(v) denotes the number of edges of FF incident to vv. The general factor problem asks the existence of a BB-factor in a given graph. A set B(v)B(v) is said to have a {\em gap of length} pp if there exists a natural number k∈B(v)k \in B(v) such that k+1,…,k+p∉B(v)k+1, \ldots, k+p \notin B(v) and k+p+1∈B(v)k+p+1 \in B(v). Without any restrictions the general factor problem is NP-complete. However, if no set B(v)B(v) contains a gap of length greater than 11, then the problem can be solved in polynomial time and Cornuejols \cite{Cor} presented an algorithm for finding a BB-factor, if it exists. In this paper we consider a weighted version of the general factor problem, in which each edge has a nonnegative weight and we are interested in finding a BB-factor of maximum (or minimum) weight. In particular, this version comprises the minimum/maximum cardinality variant of the general factor problem, where we want to find a BB-factor having a minimum/maximum number of edges. We present an algorithm for the maximum/minimum weight BB-factor for the case when no set B(v)B(v) contains a gap of length greater than 11. This also yields the first polynomial time algorithm for the maximum/minimum cardinality BB-factor for this case

    Protein repair in Arabidopsis and the control of seed vigor

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    Does Kin Recognition and Sib-Mating Avoidance Limit the Risk of Genetic Incompatibility in a Parasitic Wasp?

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    Background: When some combinations of maternal and paternal alleles have a detrimental effect on offspring fitness, females should be able to choose mates on the basis of their genetic compatibility. In numerous Hymenoptera, the sex of an individual depends of the allelic combination at a specific locus (single-locus Complementary Sex Determination), and in most of these species individuals that are homozygous at this sexual locus develop into diploid males with zero fitness. Methods and Findings: In this paper, we tested the hypothesis of genetic incompatibility avoidance by investigating sibmating avoidance in the solitary wasp parasitoid, Venturia canescens. In the context of mate choice we show, for the first time in a non-social hymenopteran species, that females can avoid mating with their brothers through kin recognition. In ‘‘no-choice’ ’ tests, the probability a female will mate with an unrelated male is twice as high as the chance of her mating with her brothers. In contrast, in choice tests in small test arenas, no kin discrimination effect was observed. Further experiments with male extracts demonstrate that chemical cues emanating from related males influence the acceptance rate of unrelated males. Conclusions: Our results are compatible with the genetic incompatibility hypothesis. They suggest that the female wasps recognize sibs on the basis of a chemical signature carried or emitted by males possibly using a ‘‘self-referent phenotyp

    Wood Utilization Is Dependent on Catalase Activities in the Filamentous Fungus Podospora anserina

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    Catalases are enzymes that play critical roles in protecting cells against the toxic effects of hydrogen peroxide. They are implicated in various physiological and pathological conditions but some of their functions remain unclear. In order to decipher the role(s) of catalases during the life cycle of Podospora anserina, we analyzed the role of the four monofunctional catalases and one bifunctional catalase-peroxidase genes present in its genome. The five genes were deleted and the phenotypes of each single and all multiple mutants were investigated. Intriguingly, although the genes are differently expressed during the life cycle, catalase activity is dispensable during both vegetative growth and sexual reproduction in laboratory conditions. Catalases are also not essential for cellulose or fatty acid assimilation. In contrast, they are strictly required for efficient utilization of more complex biomass like wood shavings by allowing growth in the presence of lignin. The secreted CATB and cytosolic CAT2 are the major catalases implicated in peroxide resistance, while CAT2 is the major player during complex biomass assimilation. Our results suggest that P. anserina produces external H2O2 to assimilate complex biomass and that catalases are necessary to protect the cells during this process. In addition, the phenotypes of strains lacking only one catalase gene suggest that a decrease of catalase activity improves the capacity of the fungus to degrade complex biomass

    Comparative transcriptome analysis reveals different strategies for degradation of steam-exploded sugarcane bagasse by Aspergillus niger and Trichoderma reesei

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    Reflexion measurements for inverse characterization of steel diffusion bond mechanical properties

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    Conference of 43rd Annual Review of Progress in Quantitative Nondestructive Evaluation, QNDE 2016 ; Conference Date: 17 July 2016 Through 22 July 2016; Conference Code:126650International audienceThe present work describes a non-destructive testing method aimed at securing high manufacturing quality of the innovative compact heat exchanger developed under the framework of the CEA R&D program dedicated to the Advanced Sodium Technological Reactor for Industrial Demonstration (ASTRID). The heat exchanger assembly procedure currently proposed involves high temperature and high pressure diffusion welding of stainless steel plates. The aim of the non-destructive method presented herein is to characterize the quality of the welds obtained through this assembly process. Based on a low-frequency model developed by Baik and Thompson [1], pulse-echo normal incidence measurements are calibrated according to a specific procedure and allow the determination of the welding interface stiffness using a nonlinear fitting procedure in the frequency domain. Performing the characterization of plates after diffusion welding using this method allows a useful assessment of the material state as a function of the diffusion bonding process
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