Isoenergetic-practical and semi-purified diets for protein requirement determination in Hermetia illucens larvae: consequences on life history traits

BACKGROUND: Black soldier fly (BSF) is one of the most promising species for the intensive breeding of insects given its adaptability and its efficiency in the conversion of waste. To maximize the production and use waste as substrates, it is essential to determine the larvae nutritional requirements. The study aims to evaluate the effects of 5 practical, semi-purified and isoenergetic diets (PSPID) with increasing protein levels (10%, CP10; 14%, CP14; 16%, CP16; 19%, CP19) on BSF life history traits. A total of 2000 six-day-old larvae were weighed and divided into groups of 100 (4 replicates/treatment [PSPID and Gainesville diet (GA) used as environmental control]). In larva-prepupa stage, sampling was carried out on 30 individuals/replicate, while in prepupa-adult stage on 35. RESULTS: In the whole larval stage, the CP16 treatment performed better when compared to the other groups. At 18 days old, the CP14 treatment showed a higher weight than the CP19 (P < 0.01), while the CP10 and CP16 weights were intermediate. On the contrary, the CP10 prepupae weight was higher than the CP19 (P < 0.01). The CP10 and CP14 pupae performed better in terms of weight when compared to the others (P < 0.01). The dietary treatments did not affect the adult parameters. The sex significantly influenced both the exuvia weight, which was the greatest in females (P < 0.001), and the fly lifespan, longest in males (P < 0.05). Fly lifespan was also influenced by the interaction between treatment and sex, with the CP19 females showing a longer life than the others (P < 0.05). CONCLUSIONS: In conclusion, the optimal protein level considering the whole larval stage is 16% on dry matter (DM). At 18 days old, looking at the size intended for the meal production, it can be considered 14% on DM. The result obtained on adult emergence in PSPID may not be attributable to the protein content. Further researches on macronutrients requirements determination have to be conducted to evaluate the substrates composition effects on BSF life history traits

Determination of lipid requirements in black soldier fly through semi-purified diets

The insect market is still far from an effective upscale and, to achieve this goal, it is necessary to know the BSF dietary requirements for the production maximization. Worldwide, given the waste variability, is not always easy to identify the optimal waste-based mixture that can allow to reach the best production, in terms of quantity and quality. Due this reason, nutritional need ranges are the basic knowledge, affordable for everyone, to increase the profitability of the insect farming. The study aims to evaluate the effects of 6 semi-purified, isonitrogenous and isoenergetic diets (SPII) with increasing lipid levels (1%, L1; 1.5%, L1.5; 2.5%, L2.5; 3.5% L3.5; 4.5%, L4.5) on BSF life history traits (6 replicates/treatment and 100 larvae/replicate). The Gainesville diet was used as environmental control. Considering the whole larval stage, 4.5% lipid level guarantees better performance when compared to content lower than 2.5%. The L4.5 10-day-old larvae yielded greater when compared to the other dietary treatments. At 14 and 18 days of age, the larvae of the groups above 2.5% performed better than L1, while the L1.5 showed intermediate results. Lipid levels below 1.5% on DM, when compared to 4.5%, resulted in a smaller prepupa and pupa size. The results obtained on the adult stage do not allow the identification of a lipid levels ideal range, as in the larval stage. In conclusion, in the whole larval stage and in prepupae/pupae phases, lipid percentage lower than (or equal to) 1% have a negative effect on growth. Other research will be needed in order to evaluate lipid levels above 4.5% on DM

Lifetime prediction of self-healing ceramic-matrix composites using a multi-physics image-based model

Please click Additional Files below to see the full abstrac

Behavioural analysis of interactions between pedestrians and vehicles in street designs with elements of shared space

This paper describes the development and implementation of qualitative behavioural criteria in order to analyse the conduct of pedestrians and vehicles when they are required to interact with each other, with particular interest to street designs with elements of shared space. The new behavioural analysis technique is developed by identifying the fundamental principles that underpin existing traffic analyses, such as traffic conflicts techniques, and adapting those to a qualitative framework that describes the mindset and rationale of road users. The technique is then applied to a case study in London, using video data from periods before and after the redevelopment of the Exhibition Road site from a conventional dual carriageway to a modern design with some elements of shared space. With the main goals being to assess the pedestrians’ confidence and the vehicles’ tolerance/patience when forced to interact with each other, behavioural trends are related to instantaneous characteristics of the vehicle flow (vehicle approach speed and traffic density). The data produced are used to develop and validate qualitative behavioural relationships for pedestrian-vehicle interactions, as well as location-specific conclusions for the Exhibition Road site

Behavioural analysis of vehicle-pedestrian interactions: The case of street designs with elements of shared space

This paper describes the development and implementation of qualitative behavioural criteria in order to analyse the conduct of pedestrians and vehicles when they are required to interact with each other, with particular interest to street designs with elements of shared space. The new behavioural analysis technique is developed by identifying the fundamental principles that underpin existing traffic analyses, such as traffic conflicts techniques, and adapting those to a qualitative framework that describes the mindset and rationale of road users. The technique is then applied to a case study in London, using video data from periods before and after the redevelopment of the Exhibition Road site from a conventional dual carriageway to a modern design with some elements of shared space. With the main goals being to assess the pedestrians’ confidence and the vehicles’ tolerance/patience when forced to interact with each other, behavioural trends are related to instantaneous characteristics of the vehicle flow (vehicle approach speed and traffic density). The data produced are used to develop and validate qualitative behavioural relationships for pedestrian-vehicle interactions, as well as location-specific conclusions for the Exhibition Road site

Osimertinib in patients with advanced epidermal growth factor receptor T790M mutation-positive non-small cell lung cancer: Rationale, evidence and place in therapy

The identification of epidermal growth factor receptor (EGFR) mutations represented a fundamental step forward in the treatment of advanced non-small cell lung cancer (NSCLC) as they define a subset of patients who benefit from the administration of specifically designed targeted therapies. The inhibition of mutant EGFR through EGFR-tyrosine kinase inhibitors (TKIs), either reversible, first-generation gefitinib and erlotinib, or irreversible, second-generation afatinib, has dramatically improved the prognosis of patients harboring this specific genetic alteration, leading to unexpected clinical benefit. Unfortunately, virtually all patients who initially respond to treatment develop acquired resistance to EGFR-TKIs within 9-14 months. The EGFR T790M secondary mutation has emerged as a cause of treatment failure in approximately 60% of resistant cases. To date, several compounds designed with the aim to overcome T790M-mediated resistance are under clinical investigation. The aim of this review is to discuss emerging data regarding the third-generation EGFR-TKI, osimertinib, for the treatment of EGFR T790M mutant advanced NSCLC