Effects of a synthetic bioactive peptide on neurite growth and nerve growth factor release in chondroitin sulfate hydrogels.

Previous work has revealed robust dorsal root ganglia neurite growth in hydrogels of chondroitin sulfate. In the current work, it was determined whether addition of a synthetic bioactive peptide could augment neurite growth in these matrices via enhanced binding and sequestering of growth factors. Fluorescence recovery after photobleaching studies revealed that addition of peptide slowed nerve growth factor diffusivity in chondroitin sulfate gels, but not in control gels of hyaluronic acid. Furthermore, cultures of chick dorsal root ganglia in gels of hyaluronic acid or chondroitin sulfate revealed enhanced growth in chondroitin sulfate gels only upon addition of peptide. Taken together, these results suggest a synergistic nerve growth factor-binding activity between this peptide and chondroitin sulfate

Cast Out: Vagrancy and Homelessness in Global and Historical Perspective

Throughout history, those arrested for vagrancy have generally been poor men and women, often young, able-bodied, unemployed, and homeless. Most histories of vagrancy have focused on the European and American experiences. Cast Out: Vagrancy and Homelessness in Global and Historical Perspective is the first book to consider the shared global heritage of vagrancy laws, homelessness, and the historical processes they accompanied. In this ambitious collection, vagrancy and homelessness are used to examine a vast array of phenomena, from the migration of labor to social and governmental responses to poverty through charity, welfare, and prosecution. The essays in Cast Out represent the best scholarship on these subjects and include discussions of the lives of the underclass, strategies for surviving and escaping poverty, the criminalization of poverty by the state, the rise of welfare and development programs, the relationship between imperial powers and colonized peoples, and the struggle to achieve independence after colonial rule. By juxtaposing these histories, the authors explore vagrancy as a common response to poverty, labor dislocation, and changing social norms, as well as how this strategy changed over time and adapted to regional peculiarities. Part of a growing literature on world history, Cast Out offers fresh perspectives and new research in fields that have yet to fully investigate vagrancy and homelessness. This book by leading scholars in the field is for policy makers, as well as for courses on poverty, homelessness, and world history. Contributors: Richard B. Allen, David Arnold, A. L. Beier, Andrew Burton, Vincent DiGirolamo, Andrew A. Gentes, Robert Gordon, Frank Tobias Higbie, Thomas H. Holloway, Abby Margolis, Paul Ocobock, Aminda M. Smith, Linda Woodbridgehttps://ohioopen.library.ohio.edu/oupress/1000/thumbnail.jp

Immunologic Aspects of Perioperative Nutrition

Nutrition has proven to be of great importance for the postoperative clinical outcome. Several studies have shown that infectious complications in the surgical patient , are reduced by pre- or postoperative nourishment. We discuss cellular immunity in relation to both enteral and parenteral nutrition and present an updated literature study of current evidence. The aim of this paper is to give an overview of studies, that compare different immunological parameters in the surgical patient being nourished either enterally or parenterally

Tracking Lysosome Migration within Chinese Hamster Ovary (CHO) Cells Following Exposure to Nanosecond Pulsed Electric Fields

Above a threshold electric field strength, 600 ns-duration pulsed electric field (nsPEF) exposure substantially porates and permeabilizes cellular plasma membranes in aqueous solution to many small ions. Repetitive exposures increase permeabilization to calcium ions (Ca2+) in a dosage-dependent manner. Such exposure conditions can create relatively long-lived pores that reseal after passive lateral diffusion of lipids should have closed the pores. One explanation for eventual pore resealing is active membrane repair, and an ubiquitous repair mechanism in mammalian cells is lysosome exocytosis. A previous study shows that intracellular lysosome movement halts upon a 16.2 kV/cm, 600-ns PEF exposure of a single train of 20 pulses at 5 Hz. In that study, lysosome stagnation qualitatively correlates with the presence of Ca2+ in the extracellular solution and with microtubule collapse. The present study tests the hypothesis that limitation of nsPEF-induced Ca2+ influx and colloid osmotic cell swelling permits unabated lysosome translocation in exposed cells. The results indicate that the efforts used herein to preclude Ca2+ influx and colloid osmotic swelling following nsPEF exposure did not prevent attenuation of lysosome translocation. Intracellular lysosome movement is inhibited by nsPEF exposure(s) in the presence of PEG 300-containing solution or by 20 pulses of nsPEF in the presence of extracellular calcium. The only cases with no significant decreases in lysosome movement are the sham and exposure to a single nsPEF in Ca2+-free solution

Destruction of single species biofi lms of Escherichia coli or Klebsiella pneumoniae subsp. pneumoniae by dextranase, lactoferrin, and lysozyme

The aim of this work was to determine the destructive activity of dextranase, lactoferrin, and lysozyme, against single species biofi lms composed of either Klebsiella pneumoniae subsp. pneumoniae or Escherichia coli using the MBEC Assay. Luminescence measurements based on quantitation of the ATP present were used to determine the amount of biofi lm elimination and correlated with quantity of live bacteria present in the sample. The data were analyzed employing a two-way ANOVA and Bonferroni post-test. Treatments resulted in percentage reductions of E. coli biofi lms ranging from 73 to 98 %. Lactoferrin (40 μg/ml) produced a signifi cantly higher-percentage reduction than lysozyme (10 μg/ml) (P < 0.05), no other signifi cant differences occurred. Similar treatments resulted in percentage reductions of K. pneumoniae subsp. pneumoniae biofilms ranging from 51 to 100 %. Dextranase treatments produced a signifi cantly lower percentage reduction than all other materials (P < 0.05), no other signifi cant differences occurred. No material was capable of complete destruction of both single species biofi lms; however, low concentrations of lactoferrin and lysozyme each removed 100 % of the K. pneumoniae subsp. pneumoniae biofi lm. Low concentrations of lactoferrin or lysozyme might be benefi cial to prevent biofi lm formation by K. pneumoniae subsp. pneumoniae. [Int Microbiol 2012; 15(4):183-187

Agouti C57BL/6N embryonic stem cells for mouse genetic resources.

We report the characterization of a highly germline competent C57BL/6N mouse embryonic stem cell line, JM8. To simplify breeding schemes, the dominant agouti coat color gene was restored in JM8 cells by targeted repair of the C57BL/6 nonagouti mutation. These cells provide a robust foundation for large-scale mouse knockout programs that aim to provide a public resource of targeted mutations in the C57BL/6 genetic background

Conditional expression of the TVA receptor allows clonal analysis of descendents from Cre-expressing progenitor cells

AbstractAn understanding of the number and types of progeny produced by progenitor cells during development provides a foundation for studies of when and where cell fate determination takes place. Lineal relationships can be revealed by the identification of descendents of cells that express a recombinase, such as Cre or Flp. This method provides data concerning gene expression history, but does not provide clonal resolution among the descendents. An alternative method employs retroviral labeling, which permits the identification of clones, but does not allow for the tracking of gene expression history. Here we report a combination of these methods to circumvent each method's limitations. By employing the specificity of Cre expression, and by selecting only a subset of cells with a Cre history for retroviral infection, clones with a gene expression history can be labeled. The method utilizes a conditional allele of the avian tumor virus receptor A (TVA), which allows infection of mouse cells following Cre activity, with mammalian retroviral vectors pseudotyped with the ASLV-A envelope glycoprotein (EnvA). We quantified the efficiency and specificity of this system in vivo and in vitro. We also generated a series of retroviral vectors encoding a variety of histochemical and fluorescent reporter genes that enable the tracking of mixtures of clones, thus enabling better resolution of clonal boundaries. This method and new vectors can be used to further our understanding of the gene expression patterns of progenitor cells that make particular daughter cells, as well as provide a platform for manipulating identified subsets of developing cells

Mapping historical forest biomass for stock-change assessments at parcel to landscape scales

Understanding historical forest dynamics, specifically changes in forest biomass and carbon stocks, has become critical for assessing current forest climate benefits and projecting future benefits under various policy, regulatory, and stewardship scenarios. Carbon accounting frameworks based exclusively on national forest inventories are limited to broad-scale estimates, but model-based approaches that combine these inventories with remotely sensed data can yield contiguous fine-resolution maps of forest biomass and carbon stocks across landscapes over time. Here we describe a fundamental step in building a map-based stock-change framework: mapping historical forest biomass at fine temporal and spatial resolution (annual, 30m) across all of New York State (USA) from 1990 to 2019, using freely available data and open-source tools. Using Landsat imagery, US Forest Service Forest Inventory and Analysis (FIA) data, and off-the-shelf LiDAR collections we developed three modeling approaches for mapping historical forest aboveground biomass (AGB): training on FIA plot-level AGB estimates (direct), training on LiDAR-derived AGB maps (indirect), and an ensemble averaging predictions from the direct and indirect models. Model prediction surfaces (maps) were tested against FIA estimates at multiple scales. All three approaches produced viable outputs, yet tradeoffs were evident in terms of model complexity, map accuracy, saturation, and fine-scale pattern representation. The resulting map products can help identify where, when, and how forest carbon stocks are changing as a result of both anthropogenic and natural drivers alike. These products can thus serve as inputs to a wide range of applications including stock-change assessments, monitoring reporting and verification frameworks, and prioritizing parcels for protection or enrollment in improved management programs.Comment: Manuscript: 24 pages, 7 figures; Supplements: 12 pages, 5 figures; Submitted to Forest Ecology and Managemen