28 research outputs found
Successional patterns along soil development gradients formed by glacier retreat in the Maritime Antarctic, King George Island
The use of Brazilian vegetable oils in nanoemulsions: an update on preparation and biological applications
Arthrobacter livingstonensis sp. nov. and Arthrobacter cryotolerans sp. nov., salt- and psychrotolerant species isolated from Livingston Island, Antarctica
Isolation and characterisation of heterotrophic microorganisms from mineral soils of Livingston Island (Antarctica) and Store Koldewey (Northeast-Greenland).
Leifsonia psychrotolerans sp. nov., a psychrotolerant species of the family Microbacteriaceae from Livingston Island, Antarctica
A cold-tolerant, yellow-pigmented, Gram-positive, motile, facultatively anaerobic bacterial strain, LI1T, was isolated from a moss-covered soil from Livingston Island, Antarctica, near the Bulgarian station St. Kliment Ohridski. Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species Leifsonia kafniensis KFC-22T, Leifsonia pindariensis PON10T and Leifsonia antarctica SPC-20T, with which it showed sequence similarities of 99.0, 97.9 and 97.9â%, respectively. DNAâDNA hybridization revealed a reassociation value of 2.7â% with L. kafniensis LMG 24362T. The DNA G+C content of strain LI1T was 64.5 mol%. The growth temperature range was â6 to 28 °C, with optimum growth at 16 °C. Growth occurred in 0â5â% NaCl and at pH 4.5â9.5, with optimum growth in 1â2â% NaCl and at pH 5.5â6.5. The predominant fatty acids were anteiso-C15â:â0, C18â:â0 and iso-C15â:â0. The polar lipids were phosphatidylglycerol and diphosphatidylglycerol. Physiological and biochemical tests clearly differentiated strain LI1T from L. kafniensis. Therefore, a novel cold-tolerant species within the genus Leifsonia is proposed: Leifsonia psychrotolerans sp. nov. (type strain LI1Tâ=âDSM 22824Tâ=âNCCB 100313T).</jats:p
Solubility determination and solution thermodynamics of olmesartan medoxomil in (PEG-400 + water) cosolvent mixtures
Stability-indicating comparative methods using mekc and lc for determination of olmesartan medoxomil
A stability-indicating method using MEKC was validated for the analysis of olmesartan medoxomil in tablets. Successful separation was achieved using a fused silica capillary (40 cm x 50 ”m i.d.); background electrolyte consisted of a combination of 10 mmol L-1 borate buffer and 5 mmol L-1 anionic detergent sodium dodecyl sulfate (95:5; v/v) pH 6.5; hydrodynamic mode at 50 mBar for 5 s; 25 kV separation voltage at 25 ÂșC; and column temperature 25 ÂșC with detection at 257 nm. The proposed method, validated following ICH guidelines, was applied to the determination of this antihypertensive with good results compared with an LC method
Determination of cetirizine and its impurities in bulk and tablet formulation using a validated capillary zone electrophoretic method
Development and Validation of a Discriminating In Vitro Dissolution Method for a Poorly Soluble Drug, Olmesartan Medoxomil: Comparison Between Commercial Tablets
A dissolution test for tablets containing 40Â mg of olmesartan medoxomil (OLM) was developed and validated using both LC-UV and UV methods. After evaluation of the sink condition, dissolution medium, and stability of the drug, the method was validated using USP apparatus 2, 50Â rpm rotation speed, and 900Â ml of deaerated H2O + 0.5% sodium lauryl sulfate (w/v) at pHâ6.8 (adjusted with 18% phosphoric acid) as the dissolution medium. The model-independent method using difference factor (f1) and similarity factor (f2), model-dependent method, and dissolution efficiency were employed to compare dissolution profiles. The kinetic parameters of drug release were also investigated. The obtained results provided adequate dissolution profiles. The developed dissolution test was validated according to international guidelines. Since there is no monograph for this drug in tablets, the dissolution method presented here can be used as a quality control test for OLM in this dosage form, especially in a batch to batch evaluation