Expression of VP60 gene from rabbit haemorrhagic disease virus (RHDV) YL strain under control of the ats1A promoter in tobacco

The VP60 gene from rabbit haemorrhagic disease virus (RHDV) YL strain in Northeast of China, under control of the ats1A promoter from Rubisco small subunit genes of Arabidopsis thaliana, was introduced into the transfer deoxyribonucleic acid (T-DNA) region of plant transfer vector pCAMBIA1300 and transferred to tobacco (Nicotiana tabacum cv. Petit Havanna SR1) with Agrobacterium tumefaciens-mediated method. Polymerase chain reaction (PCR) reverse transcription(RT) and -PCR analysis of the transformed tobacco plants confirmed the integration of the VP60 gene copy into the plant DNA and VP60 gene transcription produced. Western blot analysis revealed that the VP60 protein was expressed in tobacco under control of ats1A promoter.Key words: Agrobacterium tumefaciens, rabbit haemorrhagic disease virus (RHDV), VP60 protein, ats1A promoter, plant-derived vaccine

Evaluation of Aerosol Fire Extinguishing Agent Using a Simple Diffusion Model

Aerosol fire extinguishing agents have been recognized as an effective replacement of Halon. The uniform diffusion time and the effective concentration of the aerosol fire extinguishing agent are important parameters for putting out a fire. In this work, the effective concentration is derived based on the diffusion equation, and its variation with changing diffusion coefficient and diffusion time is analyzed. The uniform diffusion time could then be conveniently estimated using this equation. Based on experimental data, the concentration kinetics of the aerosol is drawn and the relation between the diffusion coefficient and the uniform diffusion time is analyzed. It was found that the uniform diffusion time is not dependent on the shape of the closed room but dependent on the total room volume and the position of its diffusion source. This model is demonstrated as a facile tool for the convenient evaluation and reasonable application of the aerosol fire extinguishing agent by predicting the uniform diffusion times of extinguishing aerosol in closed rooms

Experimental study of THGEM detector with mini-rim

The gas gain and energy resolution of single and double THGEM detectors (5{\times}5cm2 effective area) with mini-rims (rim is less than 10{\mu}m) were studied. The maximum gain can reach 5{\times}103 and 2{\times}105 for single and double THGEM respectively, while the energy resolution of 5.9 keV X-ray varied from 18% to 28% for both single and double THGEM detectors of different hole sizes and thicknesses.All the experiments were investigated in mixture of noble gases(argon,neon) and small content of other gases(iso-butane,methane) at atmospheric pressure.Comment: 4pages,6figures, it has been submitted to Chinese Physics

Perspective of monochromatic gamma-ray line detection with the High Energy cosmic-Radiation Detection (HERD) facility onboard China's Space Station

HERD is the High Energy cosmic-Radiation Detection instrument proposed to operate onboard China's space station in the 2020s. It is designed to detect energetic cosmic ray nuclei, leptons and photons with a high energy resolution ($\sim1\%$ for electrons and photons and $20\%$ for nuclei) and a large geometry factor ($>3\,{ m^2\,sr}$ for electrons and diffuse photons and $>2\,{ m^2\,sr}$ for nuclei). In this work we discuss the capability of HERD to detect monochromatic $\gamma$-ray lines, based on simulations of the detector performance. It is shown that HERD will be one of the most sensitive instruments for monochromatic $\gamma$-ray searches at energies between $\sim10$ to a few hundred GeV. Above hundreds of GeV, Cherenkov telescopes will be more sensitive due to their large effective area. As a specific example, we show that a good portion of the parameter space of a supersymmetric dark matter model can be probed with HERD.Comment: 9 pages, 7 figures, matches version published in Astropart.Phy