High-intensity interval training combined with vibration and dietary restriction Improves body composition and blood lipids in obese adults: a randomized trial

This study aimed to compare the effect of high-intensity interval training (HIIT) with additional whole-body vibration (WBV) on body composition and lipid profile in obese/overweight adults on a hypocaloric diet. Forty adults were randomly assigned to (a) HIIT and vibration and hypocaloric diet (HIITWBV, n ¼ 13), (b) HIIT and diet (HIIT, n ¼ 14), and (c) diet only (control [CON], n ¼ 13). High-intensity interval training WBV participants trained 3 times per week for 8 weeks (6 sets 1 minute of HIIT, cycling at 90% heart rate peak followed by 1 minute of interset vibration, at a frequency of 18 Hz increasing until 25 Hz with a peak-to- peak displacement of 4 mm. Training volume increased 1 set every 2 weeks until 10 sets). The HIIT group performed HIIT training followed by 2 minutes of passive recovery, while the CON continued with their daily activities combined with calorie restriction. Body composition (body fat and fat-free mass) and biochemical indices (glucose, total cholesterol, high-density lipoprotein cholesterol, and triglycerides) were determined. Following 8 weeks, body fat was significantly reduced by 7.5% and both tri- glycerides and total cholesterol decreased in the HIITWBV group only ( 16.5% and 11.7% respectively). This study suggests that HIIT in combination with WBV and a hypocaloric diet can improve overall lipid profile in overweight/obese individual

Effect of Bioorganic Enhancers on Bean (Phaseolus vulgaris L.) Yields

This research took place on El Huerto Farm, Cándido González Cooperative of Credit and Services, in the province of Camaguey, Cuba, on low-natural-fertility, red-brown, mulled magnesian ferrallitic soil. The goal of the study was to increase yields of bean variety "Rosa", using bio-organic enhancers applied every seven days after seed germination. A randomized block design was made, with six treatments and four replicas. The indicators evaluated were, number of pod per plants, number of beans per pod, volume of 100 beans, and crop yields. A computerized two-way analysis of variance was used for evaluation of the experimental data; the Duncan´s test was applied to significant values (5%). The treatment with natural liquid humus + potassium nitrate + potassium sulfate produced the highest rise in yields and related componentsThis research took place on El Huerto Farm, Cándido González Cooperative of Credit and Services, in the province of Camaguey, Cuba, on low-natural-fertility, red-brown, mulled magnesian ferrallitic soil. The goal of the study was to increase yields of bean variety "Rosa", using bio-organic enhancers applied every seven days after seed germination. A randomized block design was made, with six treatments and four replicas. The indicators evaluated were, number of pod per plants, number of beans per pod, volume of 100 beans, and crop yields. A computerized two-way analysis of variance was used for evaluation of the experimental data; the Duncan´s test was applied to significant values (5%). The treatment with natural liquid humus + potassium nitrate + potassium sulfate produced the highest rise in yields and related component

Effect of Bioorganic Enhancers on Bean (Phaseolus vulgaris L.) Yields

This research took place on El Huerto Farm, Cándido González Cooperative of Credit and Services, in the province of Camaguey, Cuba, on low-natural-fertility, red-brown, mulled magnesian ferrallitic soil. The goal of the study was to increase yields of bean variety "Rosa", using bio-organic enhancers applied every seven days after seed germination. A randomized block design was made, with six treatments and four replicas. The indicators evaluated were, number of pod per plants, number of beans per pod, volume of 100 beans, and crop yields. A computerized two-way analysis of variance was used for evaluation of the experimental data; the Duncan´s test was applied to significant values (5%). The treatment with natural liquid humus + potassium nitrate + potassium sulfate produced the highest rise in yields and related components

Efecto de potenciadores bioorgánicos sobre el rendimiento del frijol (Phaseolus vulgaris L.)

La investigación se realizó en la finca “El Huerto” perteneciente a la Cooperativa de Créditos y Servicios “Cándido González” en la provincia de Camagüey-Cuba, sobre un suelo Fersialítico Rojo Parduzco Mullido Ferromagnesial de baja fertilidad natural, teniendo como objetivo incrementar el rendimiento en el cultivo del frijol variedad Rosa, con el empleo de potenciadores bioorgánicos, los que se aplicaron cada siete días a partir de los diez días de germinada la semilla. El diseño experimental utilizado fue de bloques al azar con seis tratamientos y cuatro réplicas. Los indicadores evaluados fueron la altura de la planta, número de vainas por plantas, número de granos por vaina, masa de 100 granos y el rendimiento agrícola. Para la evaluación de los datos experimentales se empleó un análisis de varianza de clasificación doble, por medio del paquete estadístico y donde hubo significación se aplicó la prueba de rangos múltiples de Duncan para un nivel de significación del 5%. Se obtuvo como resultado que en el tratamiento donde se aplica el humus líquido natural + nitrato de potasio + sulfato de potasio, se logra el mayor incremento en el rendimiento y sus principales componentes

Efecto de potenciadores bioorgánicos sobre el rendimiento del frijol (Phaseolus vulgaris L.)

La investigación se realizó en la finca “El Huerto” perteneciente a la Cooperativa de Créditos y Servicios “Cándido González” en la provincia de Camagüey-Cuba, sobre un suelo Fersialítico Rojo Parduzco Mullido Ferromagnesial de baja fertilidad natural, teniendo como objetivo incrementar el rendimiento en el cultivo del frijol variedad Rosa, con el empleo de potenciadores bioorgánicos, los que se aplicaron cada siete días a partir de los diez días de germinada la semilla.  El diseño experimental utilizado fue de bloques al azar con seis tratamientos y cuatro réplicas. Los indicadores evaluados fueron la altura de la planta, número de vainas por plantas, número de granos por vaina, masa de 100 granos y el rendimiento agrícola. Para la evaluación de los datos experimentales se empleó un análisis de varianza de clasificación doble, por medio del paquete estadístico y donde hubo significación se aplicó la prueba de rangos múltiples de Duncan para un nivel de significación del 5%. Se obtuvo como resultado que en  el tratamiento donde se aplica el humus líquido natural + nitrato de potasio + sulfato de potasio, se logra el mayor incremento en el rendimiento y sus principales componentes ABSTRACTThis research took place on El Huerto Farm, Cándido González Cooperative of Credit and Services, in the province of Camaguey, Cuba, on low-natural-fertility, red-brown, soft fersiallitic ferromagnesial soil. The goal of the study was to increase yields of bean variety "rosa", using bioorganic primers, applied every seven days after seed germination. A randomized block design was made, with six treatments and four replicas. The indicators evaluated were, number of pod per plants, number of bean per pod, mass of 100 beans, and agricultural yield. A computerized double variance analysis was used for evaluation of the experimental data; the Duncan´s test was applied to significant instances of 5%. The treatment that applied the natural liquid humus + potassium nitrate + potassium sulphate produced the highest rise in yields and related components

Photopolymerization of ionic liquids in flexible microporous aramids for ion conductive solid polyelectrolytes

This work presents the preparation of novel solid polymer electrolytes based on flexible microporous aramids filled with photopolymerized ionic liquids and lithium salt. The materials combined a high ionic conductivity with the mechanical and thermal characteristics of the aramids, including also good flexibility and handleability. First, a simple casting process was followed to obtain microporous aramids with an interconnected channel morphology. In a second step, this channel structure was filled with a solution of non-commercial photopolymerizable ionic liquid, commercial ionic liquids and the lithium salt, followed by UV irradiation to obtain the conducting aramids. Ionic conductivity of the materials was studied at 25 °C, and also in the temperature range between −50 to 90 °C, together with SEM analyses of the filled porous structure and thermal properties, to fully characterize the photopolymerization process of the ionic liquids inside the porous structure. The materials showed high ionic conductivity values together with excellent thermal and mechanical properties, indicating their viability as flexible and thermally stable solid electrolytes.FEDER (Fondo Europeo de Desarrollo Regional) and both the Spanish Ministerio de Economía, Industria y Competitividad (MAT2017-84501-R and MAT2017-88923-P), the Consejería de Educacion-Junta ´ de Castilla y Leon ´ (BU306P18) and the Spanish Ministerio de Ciencia e Innovacion ´ (PID2019-108583RJ-I00/AEI/10.13039/501100011033)

Antibacterial potential of commercial and wild lactic acid bacteria strains isolated from ovine and caprine raw milk against Mycoplasma agalactiae

© 2023 Toquet, Bataller, Gomis, Sánchez, Toledo-Perona, De la Fe, Corrales and Gómez-Martín. This is an open-access article distributed under the terms of the Creative Commons CC-BY license, http://creativecommons.org/licenses/by /4.0/ This document is the Published version of a Published Work that appeared in final form in Frontiers in Veterinary Science. To access the final edited and published work see https://doi.org/10.3389/fvets.2023.1197701Introduction: The complexity of fighting contagious agalactia (CA) has raised the necessity of alternative antimicrobial therapies, such as probiotics. Lactic acid bacteria (LAB) are present in the mammary gland of small ruminants and their antimicrobial effect have been previously described against species like Mycoplasma bovis but never against Mycoplasma agalactiae (Ma). This in vitro study aims to evaluate the antimicrobial activity against Ma of ovine and caprine LAB strains and a human commercial probiotic (L2) of Lactobacillus spp. Methods: A total of 63 possible LAB strains were isolated from nine ovine and caprine farms in Spain, three isolates (33B, 248D, and 120B) from the 63 strains were selected, based on their capacity to grow in a specific medium in vitro, for an in vitro experiment to assess their antimicrobial activity against Ma in Ultra High Temperature (UHT) processed goat milk (GM). A women commercial vaginal probiotic was also included in the study. The inoculum of L2 was prepared at a concentration of 3.24 × 108  CFU/mL and the average concentration of the inoculum of the wild LAB varied from 7.9 × 107 to 8.4 × 108  CFU/mL. Results: The commercial probiotic L2 significantly reduced the concentration of Ma to 0.000 log CFU/mL (p < 0.001), strain 33B reduced it from 7.185 to 1.279 log CFU/mL (p < 0.001), and 120B from 6.825 to 6.466 log CFU/mL (p < 0.05). Strain 248D presented a bacteriostatic effect in GM. Moreover, the three wild strains and the commercial probiotic produced a significative reduction of the pH (p < 0.001). Discussion: This is the first in vivo report of the antimicrobial potential of LAB strains against Ma and its interaction. Our results support possible future alternative strategies to antibiotic therapy, previously not contemplated, to fight CA in small ruminants. Further studies are necessary to elucidate the action mechanisms through which these LAB are able to inhibit Ma and to assess the safety of using these strains in possible in vivo studies

Production of a functionally active recombinant SARS-CoV-2 (COVID-19) 3C-Like protease and a soluble inactive 3C-like protease-RBD chimeric in a prokaryotic expression system

During the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) intracellular life-cycle, two large polyproteins, pp1a and pp1ab, are produced. Processing of these by viral cysteine proteases, the papain-like protease (PLpro) and the chymotrypsin-like 3C-like protease (3CL-pro) release non-structural proteins necessary for the establishment of the viral replication and transcription complex (RTC), crucial for viral replication. Hence, these proteases are considered prime targets against which anti-coronavirus disease 2019 (COVID-19) drugs could be developed. Here, we describe the expression of a highly soluble and functionally active recombinant 3CL-pro using Escherichia coli BL21 cells. We show that the enzyme functions in a dimeric form and exhibits an unexpected inhibitory profile because its activity is potently blocked by serine rather than cysteine protease inhibitors. In addition, we assessed the ability of our 3CL-pro to function as a carrier for the receptor binding domain (RBD) of the Spike protein. The co-expressed chimeric protein, 3CLpro-RBD, did not exhibit 3CL-pro activity, but its enhanced solubility made purification easier and improved RBD antigenicity when tested against serum from vaccinated individuals in ELISAs. Chimeric proteins containing the 3CL-pro could represent an innovative approach to developing new COVID-19 vaccines