Evaluation of Wedged Arterial Injection as a New Technique for Delivery of Experimental Therapeutic Sustances into the Porcine Pancreas

Objectives. To prospectively evaluate the technical feasibility and efficacy of wedged arterial injection (WAI) as a potential route for experimental selective therapy to the pancreas of healthy pigs. Materials and Methods. Selective angiographies were completed in ten pigs under general anaesthesia. By superselective angiography, the catheter was inserted and wedged into the major pancreatic artery, blocking the blood flow. In order to evaluate the efficacy of the WAI method, a DNA-specific fluorescent dye (Hoechst 33258) was used. Results. Histological study revealed a uniform distribution of the fluorescent dye within the nuclei of the endocrine and exocrine pancreatic cells. Pancreatic and liver enzymes as well as histopathology of the pancreas were normal. Conclusion. WAI is a highly effective minimally invasive methodology to target the porcine pancreas. The findings suggest that WAI may contribute to developing preclinical assays of pancreas gene or cell-transfer therapies in swine model

Efecto de la triploidía sobre los parámetros musculares y nutricionales del rodaballo (Scophthalmus maximus, L.) después de su maduración sexual

Con el objetivo de una mejor comprensión de la calidad final del rodaballo triploide (Scophtha/mus maximus, L.), la celularidad musculary los parámetros de calidad de la carne fueron estudiados en 7 rodaballos diploides y 7 lriploides de 33 meses de edad, todos ejemplares decultivoy de un tamaño similar. La celularidad del músculo blanco se evaluó por medio del número y diámetro de las fibras musculares. Para determinarla calidad de la carne fueron analizados diversos parámetros físico-químicos (humedad, proteína, grasa y ácidos grasas totales) y se determinaIOO las propiedades mecánicas de la lextura (dureza, elasticidad, masticabilidad, cohe~ividad y gomosidad) con un texturómetro. El mecanismode crecimiento muscular difirió entre los dos grupos, de tal manera que el tamaño de las fibras fue mayor (p<O,OOI)en los triploides , mientrasquela densidad de fibras musculares fue mayor en el grupo de los diploides, lo que demuestra que las diferencias genéticas produjeron dtlerente celularidad muscular en ambos grupos. No se encontraron diferencias significativas en las propiedades texturales ni en los parámetros decalidad de la carne entre diploides y triploides.In order lo a beller understanding of Ihe final qualily of lriploid turbot (Scophthalmus maximus, L.), muscle cellularily and qualily parametersoflhe flesh were studied on 7 diploid and 7 triploid 33 months old farmed turbot of similar size. White muscle cellularily was evaluated by meansofIhe number and diameter of muscle fibers. lo ascertain the flesh qualily, several physlco-cbemlcal parameters (moisture, protein, total fat andfalty acids) were analyzed, and textural mechanical properties (hardness, springiness, chewiness, cohesiveness, gumminess) were determinedwitha texturometer. Muscle growth mechanism differed between both groups, such that muscie fibres size was greater (p<O,OOI)in triploid specimens, wihereas muscle fibre density was higher in diploid group, thus showing mat genetic differences produced different muscie cellularity in bothgroups. No significant differences were found in textural properties and flesh qualily parameters between diploid and triploid groups

Diferenciación histoquímica de los tipos de fibras del músculo semitendinoso del perro [Microforma].

Tesis - Universidad de Murcia.NEBRIJA. MEDIATECA. MF 37 1471

Estructura, ultraestructura y dinámica de crecimiento de la musculatura lateral de la lubina (Dicentrarchus labrax, L.) y la dorada (sparus aurata) durante el desarrollo larvario y postlarvario [Microforma] .

Tesis-Universidad de Murcia.Consulte la tesis en: BCA. GENERAL. MEDIATECA. MF-TD 1393.Consulte la tesis en: BCA. GENERAL. MEDIATECA. MF-TD 1369.NEBRIJA. MEDIATECA. MF-24-1070.NEBRIJA. MEDIATECA. MF 37 1474

Estructura, ultraestructura y dinámica de crecimiento de la musculatura lateral de la lubina (Dicentrarchus labrax L.) y la dorada (Sparus aurata L.) durante el desarrollo larvario y postlarvario / Octavio M. López Albors ; director Francisco Moreno Medina.

Tesis-Universidad de Murcia.Consulte la tesis en: BCA. GENERAL. ARCHIVO UNIVERSITARIO. T.M.-941.Consulte la tesis en: BCA. GENERAL. Fac. Veterinaria. Sala de estudio. Tesis-V 62

Influencia de la temperatura sobre el crecimiento muscular de la lubina (Dicentrarchus Labrax L.) durante el desarrollo larvario / Mª Dolores Ayala Florenciano ; directores Octavio M. López Albors, Francisco Gil Cano.

Tesis-Universidad de Murcia.Consulte la tesis en: BCA. GENERAL. DEPOSITO. T.M-2027.Consulte la tesis en: BCA. GENERAL. Fac. Veterinaria. Sala de estudio. Tesis-V 122

The histochemical profiles of fibre types in porcine skeletal muscle

Using a variety of histochemical methods -mATPase staining after alkaline and acid preincubations, NADH-TR and a-MGPDH- we have investigated the fibre types in porcine skeletal muscle. The results reveal that four major fibre types -1, IIA, IIB and II*- can be separated histochemically in Longissimus lumborum muscle of Landrace pigs. The histochemical properties of the muscle fibre type 11* are very similar to that of type IIX described in other mammals. The existence of IIX fibres in pig muscle has been recently demonstrated by molecular biology techniques and our results validate the use of histochemistry (mATPase) as an easy methodology to differentiate the three fast myosins (type 11 fibres) in pig muscle

Plastinated macroparasites, an alternative resource for use in practical lessons

© 2020. OIE.. This document is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the published version of a published Work that appeared in final form in Revue scientifique et technique - Office international des épizooties,.The plastination technique is a recently developed option to preserve biological tissues widely used in human and veterinary anatomy. This process allows one to obtain real, safe, clean, dry, resistant and permanently stable material without any additional treatment or maintenance. All these advantages are associated with the rapid expansion of plastination during recent decades in a high number of educational institutions. However, the studies of parasite conservation through plastination are scarce, although the material obtained has a high quality, as prior references have shown. In this study, 141 students of veterinary medicine and pharmacy university degrees used, in a blind study, plastinated specimens (experimental group) or wet specimens (control group) during three consecutive practical sessions. Afterwards all the students completed anonymous questionnaires on both knowledge and satisfaction. The results demonstrated that plastinated parasites are a valid and satisfactory alternative to be used as a teaching/learning tool during practical lessons. Moreover, with regard to the knowledge of morphological structures, the results from the questionnaire of assimilated knowledge showed no differences between the use of plastinated and formaldehyde-preserved parasite