503 research outputs found
Algorithms for fitting cylindrical objects to sparse range point clouds for rapid workspace modeling
Toxicity of Aromatic Plants and Their Constituents Against Coleopteran Stored Products Insect Pests
Evaluation of genetic diversity and linkage disequilibrium in Korean-bred rice varieties using SSR markers
Background: In order to evaluate the variation among different rice
types, the genetic diversity in a rice collection composed by 59
breeding lines, 23 landraces, 18 weedy rice lines, and 35 introduced
lines that collected from countries worldwide was analyzed using 134
simple sequence repeat markers. Results: In total, 1264 alleles were
identified (average, 9.43 per locus). Rare alleles made up a large
portion (58.4%) of the detected alleles, and 29 unique alleles
associated with rice accessions were also discovered. A model-based
structural analysis revealed the presence of three subpopulations. The
genetic relationships revealed by the neighbour-joining tree method
were fairly consistent with the structure-based membership assignments
for most of the accessions. A total of 105 accessions (79.5%) showed a
clear relationship to each cluster, while the remaining 27 accessions
(20.5%) were categorized as admixtures. Linkage disequilibrium (LD)
patterns and distributions are of fundamental importance for
genome-wide association mapping. The mean r2 value for all
intrachromosomal loci pairs was 0.1286. The LD between linked markers
decreased with the genetic distance between pairs of linked loci.
Conclusions: These results will provide an effective aid for future
allele mining, association genetics, mapping and cloning gene(s),
germplasm conservation, and improvement programs
Development of cDNA-derived SSR markers and their efficiency in diversity assessment of Cymbidium accessions
Abstract Cymbidium spp. are popular flowering plants. Assessment of
the genetic diversity in cultivated Cymbidium facilitates conservation
of germplasm and subsequent cultivar improvement. Thus, it is important
to develop more efficient polymorphic DNA markers. Although more motifs
(403) were identified and more primers (206) were designed in the
genomic library compared to the cDNA library, a larger number of
successful primers were obtained from the cDNA library (59.9%) than
from genomic DNA library (51.1%). However, higher PIC and gene
diversity were identified in genomic SSRs. The average allele number
per locus was also higher in genomic SSRs (7.3) than EST-SSRs (5.2),
among the 24 evaluated Cymbidium accessions. AT/TA was comparatively
high in EST-SSRs, while this motif was not as common in genomic SSRs.
The CTT/AAG/TCT/AGA/TTC/GAA and TGC/GCA/GCT/AGC/CTG/CAG motifs were the
most abundant tri-nucleotide sequences in EST-SSRs, while
GTT/AAC/TGT/ACA/TTG/CAA was the most frequent in genomic SSRs. The
number of repeats ranged from 3 to 12 in EST-SSRs. Currently, 52 novel
polymorphic SSR markers have been evaluated, which will be useful for
germplasm assessments, core set construction, evaluation of genetic
diversity, and marker assisted selection (MAS) based Cymbidium
breeding
Night shift work and inflammatory markers in male workers aged 20â39 in a display manufacturing company
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