Development and application of a self-referencing glucose microsensor for the measurement of glucose consumption by pancreatic ?-cells

Glucose gradients generated by an artificial source and ?-cells were measured using an enzyme-based glucose microsensor, 8-?m tip diameter, as a self-referencing electrode. The technique is based on a difference measurement between two locations in a gradient and thus allows us to obtain real-time flux values with minimal impact of sensor drift or noise. Flux values were derived by incorporation of the measured differential current into Fick's first equation. In an artificial glucose gradient, a flux detection limit of 8.2 ± 0.4 pmol·cm-2·s-1 (mean ± SEM, n = 7) with a sensor sensitivity of 7.0 ± 0.4 pA/mM (mean ± SEM, n = 16) was demonstrated. Under biological conditions, the glucose sensor showed no oxygen dependence with 5 mM glucose in the bulk medium. The addition of catalase to the bulk medium was shown to ameliorate surface-dependent flux distortion close to specimens, suggesting an underlying local accumulation of hydrogen peroxide. Glucose flux from ?-cell clusters, measured in the presence of 5 mM glucose, was 61.7 ± 9.5 fmol·nL-1·s-1 (mean ± SEM, n = 9) and could be pharmacologically modulated. Glucose consumption in response to FCCP (1 ?M) transiently increased, subsequently decreasing to below basal by 93 ± 16 and 56 ± 6%, respectively (mean ± SEM, n = 5). Consumption was decreased after the application of 10 ?M rotenone by 74 ± 5% (mean ± SEM, n = 4). These results demonstrate that an enzyme-based amperometric microsensor can be applied in the self-referencing mode. Further, in obtaining glucose flux measurements from small clusters of cells, these are the first recordings of the real-time dynamic of glucose movements in a biological microenvironment. <br/

Evaluating the Diversity of Emergency Medicine Foundation (EMF) Grant Recipients in the Last Decade

On behalf of the ACEP Research CommitteeIntroduction: To study diversity of researchers and barriers to success among Emergency Medicine Foundation (EMF) grant recipients in the last 10 years.Methods: EMF grant awardees were approached to complete a brief survey, which included demographics, queries related to contributions to the literature, success in obtaining grants, and any perceived barriers they encountered.Results: Of the 342 researchers contacted by email, a total of 147 completed the survey for a response rate of 43%. The respondents were predominately mid to late career white-male-heterosexual-Christian with an average age of 44 years (range 25-69 years of age). With regards to training and education, the majority of respondents (50%) were either Associate or Professor clinical rank (8% instructor/resident/fellow and 31% Assistant). Sixty-two percent of the respondents reported perceived barriers to career advancement since completion of residency. The largest perceived barrier to success was medical specialty (26%), followed by gender (21%) and age (16%).Conclusion: Our survey of EMF grant recipients in the last 10 years shows a considerable lack of diversity. The most commonly perceived barriers to career advancement by this cohort were medical specialty, gender, and age. An opportunity exists for further definition of barriers and development of mechanisms to overcome them, with a goal of increased success for those that are underrepresented. 

Two Bipolar Outflows and Magnetic Fields in a Multiple Protostar System, L1448 IRS 3

We performed spectral line observations of CO J=2-1, 13CO J=1-0, and C18O J=1-0 and polarimetric observations in the 1.3 mm continuum and CO J=2-1 toward a multiple protostar system, L1448 IRS 3, in the Perseus molecular complex at a distance of ~250 pc, using the BIMA array. In the 1.3 mm continuum, two sources (IRS 3A and 3B) were clearly detected with estimated envelope masses of 0.21 and 1.15 solar masses, and one source (IRS 3C) was marginally detected with an upper mass limit of 0.03 solar masses. In CO J=2-1, we revealed two outflows originating from IRS 3A and 3B. The masses, mean number densities, momentums, and kinetic energies of outflow lobes were estimated. Based on those estimates and outflow features, we concluded that the two outflows are interacting and that the IRS 3A outflow is nearly perpendicular to the line of sight. In addition, we estimated the velocity, inclination, and opening of the IRS 3B outflow using Bayesian statistics. When the opening angle is ~20 arcdeg, we constrain the velocity to ~45 km/s and the inclination angle to ~57 arcdeg. Linear polarization was detected in both the 1.3 mm continuum and CO J=2-1. The linear polarization in the continuum shows a magnetic field at the central source (IRS 3B) perpendicular to the outflow direction, and the linear polarization in the CO J=2-1 was detected in the outflow regions, parallel or perpendicular to the outflow direction. Moreover, we comprehensively discuss whether the binary system of IRS 3A and 3B is gravitationally bound, based on the velocity differences detected in 13CO J=1-0 and C18O J=1-0 observations and on the outflow features. The specific angular momentum of the system was estimated as ~3e20 cm^2/s, comparable to the values obtained from previous studies on binaries and molecular clouds in Taurus.Comment: ApJ accepted, 20 pages, 2 tables, 10 figure

First measurements of high frequency cross-spectra from a pair of large Michelson interferometers

Measurements are reported of the cross-correlation of spectra of differential position signals from the Fermilab Holometer, a pair of co-located 39 m long, high power Michelson interferometers with flat, broadband frequency response in the MHz range. The instrument obtains sensitivity to high frequency correlated signals far exceeding any previous measurement in a broad frequency band extending beyond the 3.8 MHz inverse light crossing time of the apparatus. The dominant but uncorrelated shot noise is averaged down over $2\times 10^8$ independent spectral measurements with 381 Hz frequency resolution to obtain $2.1\times 10^{-20} \ \mathrm{m}/\sqrt{\mathrm{Hz}}$ sensitivity to stationary signals. For signal bandwidths $\Delta f > 11$ kHz, the sensitivity to strain $h$ or shear power spectral density of classical or exotic origin surpasses a milestone $PSD_{\delta h} < t_p$ where $t_p= 5.39\times 10^{-44}/\mathrm{Hz}$ is the Planck time.Comment: 5 pages, 3 figure

Interferometric Constraints on Quantum Geometrical Shear Noise Correlations

Final measurements and analysis are reported from the first-generation Holometer, the first instrument capable of measuring correlated variations in space-time position at strain noise power spectral densities smaller than a Planck time. The apparatus consists of two co-located, but independent and isolated, 40 m power-recycled Michelson interferometers, whose outputs are cross-correlated to 25 MHz. The data are sensitive to correlations of differential position across the apparatus over a broad band of frequencies up to and exceeding the inverse light crossing time, 7.6 MHz. By measuring with Planck precision the correlation of position variations at spacelike separations, the Holometer searches for faint, irreducible correlated position noise backgrounds predicted by some models of quantum space-time geometry. The first-generation optical layout is sensitive to quantum geometrical noise correlations with shear symmetry---those that can be interpreted as a fundamental noncommutativity of space-time position in orthogonal directions. General experimental constraints are placed on parameters of a set of models of spatial shear noise correlations, with a sensitivity that exceeds the Planck-scale holographic information bound on position states by a large factor. This result significantly extends the upper limits placed on models of directional noncommutativity by currently operating gravitational wave observatories.Comment: Matches the journal accepted versio

Effects of Backflow Correlation in the Three-Dimensional Electron Gas: Quantum Monte Carlo Study

The correlation energy of the homogeneous three-dimensional interacting electron gas is calculated using the variational and fixed-node diffusion Monte Carlo methods, with trial functions that include backflow and three-body correlations. In the high density regime the effects of backflow dominate over those due to three-body correlations, but the relative importance of the latter increases as the density decreases. Since the backflow correlations vary the nodes of the trial function, this leads to improved energies in the fixed-node diffusion Monte Carlo calculations. The effects are comparable to those found for the two-dimensional electron gas, leading to much improved variational energies and fixed-node diffusion energies equal to the release-node energies of Ceperley and Alder within statistical and systematic errors.Comment: 14 pages, 5 figures, submitted to Physical Review

Domestic Pigs Have Low Susceptibility to H5N1 Highly Pathogenic Avian Influenza Viruses

Genetic reassortment of H5N1 highly pathogenic avian influenza viruses (HPAI) with currently circulating human influenza A strains is one possibility that could lead to efficient human-to-human transmissibility. Domestic pigs which are susceptible to infection with both human and avian influenza A viruses are one of the natural hosts where such reassortment events could occur. Virological, histological and serological features of H5N1 virus infection in pigs were characterized in this study. Two- to three-week-old domestic piglets were intranasally inoculated with 106 EID50 of A/Vietnam/1203/04 (VN/04), A/chicken/Indonesia/7/03 (Ck/Indo/03), A/Whooper swan/Mongolia/244/05 (WS/Mong/05), and A/Muscovy duck/Vietnam/ 209/05 (MDk/VN/05) viruses. Swine H3N2 and H1N1 viruses were studied as a positive control for swine influenza virus infection. The pathogenicity of the H5N1 HPAI viruses was also characterized in mouse and ferret animal models. Intranasal inoculation of pigs with H5N1 viruses or consumption of infected chicken meat did not result in severe disease. Mild weight loss was seen in pigs inoculated with WS/Mong/05, Ck/Indo/03 H5N1 and H1N1 swine influenza viruses. WS/Mong/05, Ck/Indo/03 and VN/04 viruses were detected in nasal swabs of inoculated pigs mainly on days 1 and 3. Titers of H5N1 viruses in nasal swabs were remarkably lower compared with those of swine influenza viruses. Replication of all four H5N1 viruses in pigs was restricted to the respiratory tract, mainly to the lungs. Titers of H5N1 viruses in the lungs were lower than those of swine viruses. WS/Mong/05 virus was isolated from trachea and tonsils, and MDk/VN/05 virus was isolated from nasal turbinate of infected pigs. Histological examination revealed mild to moderate bronchiolitis and multifocal alveolitis in the lungs of pigs infected with H5N1 viruses, while infection with swine influenza viruses resulted in severe tracheobronchitis and bronchointerstitial pneumonia. Pigs had low susceptibility to infection with H5N1 HPAI viruses. Inoculation of pigs with H5N1 viruses resulted in asymptomatic to mild symptomatic infection restricted to the respiratory tract and tonsils in contrast to mouse and ferrets animal models, where some of the viruses studied were highly pathogenic and replicated systemically

TADPOL: A 1.3 mm Survey of Dust Polarization in Star-forming Cores and Regions

We present {\lambda}1.3 mm CARMA observations of dust polarization toward 30 star-forming cores and 8 star-forming regions from the TADPOL survey. We show maps of all sources, and compare the ~2.5" resolution TADPOL maps with ~20" resolution polarization maps from single-dish submillimeter telescopes. Here we do not attempt to interpret the detailed B-field morphology of each object. Rather, we use average B-field orientations to derive conclusions in a statistical sense from the ensemble of sources, bearing in mind that these average orientations can be quite uncertain. We discuss three main findings: (1) A subset of the sources have consistent magnetic field (B-field) orientations between large (~20") and small (~2.5") scales. Those same sources also tend to have higher fractional polarizations than the sources with inconsistent large-to-small-scale fields. We interpret this to mean that in at least some cases B-fields play a role in regulating the infall of material all the way down to the ~1000 AU scales of protostellar envelopes. (2) Outflows appear to be randomly aligned with B-fields; although, in sources with low polarization fractions there is a hint that outflows are preferentially perpendicular to small-scale B-fields, which suggests that in these sources the fields have been wrapped up by envelope rotation. (3) Finally, even at ~2.5" resolution we see the so-called "polarization hole" effect, where the fractional polarization drops significantly near the total intensity peak. All data are publicly available in the electronic edition of this article.Comment: 53 pages, 37 figures -- main body (13 pp., 3 figures), source maps (32 pp., 34 figures), source descriptions (8 pp.). Accepted by the Astrophysical Journal Supplemen

Highly Ordered and Pinched Magnetic Fields in the Class 0 Protobinary System L1448 IRS 2

We have carried out polarimetric observations with the Atacama Large Millimeter/submillimeter Array toward the Class 0 protostellar system L1448 IRS 2, which is a protobinary embedded within a flattened, rotating structure, and for which a hint of a central disk has been suggested, but whose magnetic fields are aligned with the bipolar outflow on the cloud core scale. Our high-sensitivity and high- resolution (̃100 au) observations show a clear hourglass magnetic field morphology centered on the protostellar system, but the central pattern is consistent with a toroidal field indicative of a circumstellar disk; though, other interpretations are also possible, including field lines dragged by an equatorial accretion flow into a configuration parallel to the midplane. If a relatively large disk does exist, it would suggest that the magnetic braking catastrophe is averted in this system, not through a large misalignment between the magnetic and rotation axes, but rather through some other mechanisms, such as nonideal magnetohydrodynamic effects and/or turbulence. We have also found a relationship of decreasing polarization fractions with intensities and the various slopes of this relationship can be understood as multiple polarization mechanisms and/or depolarization from a changing field morphology. In addition, we found a prominent clumpy depolarization strip crossing the center perpendicular to the bipolar outflow. Moreover, a rough estimate of the magnetic field strength indicates that the field is strong enough to hinder formation of a rotationally supported disk, which is inconsistent with the feature of a central toroidal field. This also suggests that early disk formation can happen even in young stellar objects with a strong primordial magnetic field