776 research outputs found
Synergistic Formation of Radicals by Irradiation with Both Vacuum Ultraviolet and Atomic Hydrogen: A Real-Time In Situ Electron Spin Resonance Study
We report on the surface modification of polytetrafluoroethylene (PTFE) as an
example of soft- and bio-materials that occur under plasma discharge by
kinetics analysis of radical formation using in situ real-time electron spin
resonance (ESR) measurements. During irradiation with hydrogen plasma,
simultaneous measurements of the gas-phase ESR signals of atomic hydrogen and
the carbon dangling bond (C-DB) on PTFE were performed. Dynamic changes of the
C-DB density were observed in real time, where the rate of density change was
accelerated during initial irradiation and then became constant over time. It
is noteworthy that C-DBs were formed synergistically by irradiation with both
vacuum ultraviolet (VUV) and atomic hydrogen. The in situ real-time ESR
technique is useful to elucidate synergistic roles during plasma surface
modification.Comment: 14 pages, 4 figure
Cleavage of pyrene-stabilized RNA bulge loops by trans-(±)-cyclohexane-1,2-diamine
Chemical agents that cleave HIV genome can be potentially used for anti-HIV therapy. In this report, the cleavage of the upper stem-loop region of HIV-1 TAR RNA was studied in a variety of buffers containing organic catalysts. trans-(±)-Cyclohexane-1,2-diamine was found to cleave the RNA with the highest activity (31%, 37°C, 18 h). Cleavage of the RNA in trans-(±)-cyclohexane-1,2-diamine buffer was also studied when the RNA was hybridized with complementary DNAs. A pyrene-modified C3 spacer was incorporated to the DNA strand to facilitate the formation of a RNA bulge loop in the RNA/DNA duplex. In contrast, unmodified DNAs cannot efficiently generate RNA bulge loops, regardless of the DNA sequences. The results showed that the pyrene-stablized RNA bulge loops were efficiently and site-specifically cleaved by trans-(±)-cyclohexane-1,2-diamine
A DNA aptamer recognising a malaria protein biomarker can function as part of a DNA origami assembly
DNA aptamers have potential for disease diagnosis and as therapeutics, particularly when interfaced with programmable molecular technology. Here we have combined DNA aptamers specific for the malaria biomarker Plasmodium falciparum lactate dehydrogenase (PfLDH) with a DNA origami scaffold. Twelve aptamers that recognise PfLDH were integrated into a rectangular DNA origami and atomic force microscopy demonstrated that the incorporated aptamers preserve their ability to specifically bind target protein. Captured PfLDH retained enzymatic activity and protein-aptamer binding was observed dynamically using high-speed AFM. This work demonstrates the ability of DNA aptamers to recognise a malaria biomarker whilst being integrated within a supramolecular DNA scaffold, opening new possibilities for malaria diagnostic approaches based on DNA nanotechnology
Is a Priming Dose of Insulin Necessary in a Low-Dose Insulin Protocol for the Treatment of Diabetic Ketoacidosis?
OBJECTIVEâThe purpose of this study was to assess the efficacy of an insulin priming dose with a continuous insulin infusion versus two continuous infusions without a priming dose
High-pressure Synthesis and Physical Properties of New Filled Skutterudite Compound BaOs4P12
We have succeeded in synthesizing samples of new filled skutterudite compound BaOs4P12 using the high-pressure synthesis technique. The physical properties of the compound are reported for the first time. The electrical resistivity decreases with decreasing temperature, and drop sharply around 1.8K. This indicates that BaOs4P12 is a new superconductor. Before the high-pressure synthesis, using synchrotron radiation x-ray, we tried to observe synthesizing processes of BaOs4P12 in-situ at high temperature and high pressure to obtain the optimum condition for synthesis
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