Stimulatory effects of ghrelin on spontaneous contractions in the rat myometrium

WOS: 000276227500005Ghrelin, a recently isolated hormone, has been reported to have modulatory effects on smooth muscle contractility. In this study, we investigated the effects of ghrelin on spontaneous contractions in the rat uterus in vitro. Myometrium strips were removed from Wistar rats following decapitation and placed in a jacketed tissue bath. After initiation of spontaneous contractions, control contractions were recorded for 10 min and various concentrations of ghrelin were added to the tissue bath cumulatively. The Wilcoxon signed ranks test was used for statistical analysis. Application of ghrelin augmented the spontaneous contractions in myometrial strips. The mean peak amplitudes of contractions were 2.69 +/- 0.32 g (n = 6), 2.71 +/- 0.31 g (n = 6), 2.92 +/- 0.36 g (n = 6), and 3.46 +/- 0.24 g (n = 6) under control conditions and after application of 0.01 mu M, 0.1 mu M, and 1 mu M ghrelin, respectively. The mean frequencies of contractions were 5.17 +/- 0.75 (n = 6), 5.17 +/- 0.75 (n = 6), 5.33 +/- 0.48 (n = 6), and 8.0 +/- 0.77 (n = 6) under control conditions and after application of 0.01 mu M, 0.1 mu M, and 1 mu M ghrelin, respectively. The increase in amplitude and frequency of contractions was significant only after application of 1 mu M ghrelin (P < 0.05). Data from this study demonstrated that ghrelin induces spontaneous contractions in a dose-dependent manner in the rat myometrium.Firat University Scientific ResearchFirat University [FUBAP-922]This study was financially supported by Firat University Scientific Research Projects Unit (FUBAP-922)

Ghrelin’in sıçan miyometriyumundaki spontan kasılmalara uyarıcı etkileri

Son yıllarda izole edilen ghrelinin düz kas kasılmaları üzerine düzenleyici etkiye sahip olduğu belirtilmektedir. Bu çalışmada, sıçan uterusundaki spontan kasılmalara ghrelin peptidinin etkileri in vitro olarak araştırıldı. Wistar sıçanların kesilmesini takiben miyometrial stripler izole organ banyosuna yerleştirildi. Spontan kasılmaların başlamasından sonra kontrol kasılmalar 10 dakikalık kayıtlar yapıldı ve izole organ banyosuna kümülatif olarak farklı konsantrasyonda ghrelin uygulamaları yapıldı. İstatistiksel analiz için Wilcoxon signed ranks testi kullanıldı. Kontrol ve 0,01 ?M, 0,1 ?M ve 1 ?M ghrelin uygulanması ile oluşan kasılmaların amplitüdü sırasıyla 2,69 ± 0,32 g (n = 6), 2,71 ± 0,31 g (n = 6), 2,92 ± 0,36 g (n = 6) ve 3,46 ± 0,24 g (n = 6) olarak hesaplandı. Kasılmaların frekansı da sırasıyla 5,17 ± 0,75 (n = 6), 5,17 ± 0,75 (n = 6), 5,33 ± 0,48 (n = 6) ve 8,0 ± 0,77 (n = 6) olarak hesaplandı. Sadece kümülatif 1 ?M ghrelin uygulama grubunda kasılmaların hem amplitüdü hem de frekansı bakımından istatistiksel olarak anlamlı artış gözlendi (P < 0,05). Bu çalışmadaki bilgiler, sıçan miyometriyumundaki spontan kasılmaları ghrelinin indüklediğini kanıtlamıştır.Ghrelin, a recently isolated hormone, has been reported to have modulatory effects on smooth muscle contractility. In this study, we investigated the effects of ghrelin on spontaneous contractions in the rat uterus in vitro. Myometrium strips were removed from Wistar rats following decapitation and placed in a jacketed tissue bath. After initiation of spontaneous contractions, control contractions were recorded for 10 min and various concentrations of ghrelin were added to the tissue bath cumulatively. The Wilcoxon signed ranks test was used for statistical analysis. Application of ghrelin augmented the spontaneous contractions in myometrial strips. The mean peak amplitudes of contractions were 2.69 ± 0.32 g (n = 6), 2.71 ± 0.31 g (n = 6), 2.92 ± 0.36 g (n = 6), and 3.46 ± 0.24 g (n = 6) under control conditions and after application of 0.01 µM, 0.1 µM, and 1 µM ghrelin, respectively. The mean frequencies of contractions were 5.17 ± 0.75 (n = 6), 5.17 ± 0.75 (n = 6), 5.33 ± 0.48 (n = 6), and 8.0 ± 0.77 (n = 6) under control conditions and after application of 0.01 µM, 0.1 µM, and 1 µM ghrelin, respectively. The increase in amplitude and frequency of contractions was significant only after application of 1 µM ghrelin (P < 0.05). Data from this study demonstrated that ghrelin induces spontaneous contractions in a dose-dependent manner in the rat myometrium

The effect of different sowing norms on some yield traits of forage rape and ıts evaluation as a bee pasture

Bu çalışma, yemlik kolzanın farklı ekim normlarının bazı verim özelliklerine etkisi, bal arıları arasındaki karşılıklı etkileşimlerinin belirlenmesi ve yemlik kolzanın arı merası oluşturulmasında alternatif bir bitki olarak değerlendirilmesi amacı ile yürütülmüştür. Çalışmada 400, 800, 1200, 1600, 2000 ve 2400 g/da ekim normu kullanılmıştır. Bitkinin çiçeklenmesi ile birlikte haftada iki defa gözlemler alınmış ve m2 başına bal arısı sayısı, bal arılarının çiçekte kalma süresi, bitki boyu, m2 başına bitki sayısı, bitki başına çiçek sayısı, m2 başına çiçek sayısı, yan dal sayısı, bitki başına kapsül, kapsülde tohum, tohum verimi ve bin tane ağırlığı gibi özellikler ele alınmıştır. Araştırma sonucunda verim bakımından 1200-1600 g/da ekim normu öne çıkmıştır. 26 Nisan tarihi, arıların yemlik kolzayı en çok ziyaret ettiği, bitkinin en yüksek boyuna ulaştığı, bitki başına ve m2 başına en fazla çiçeğe sahip olduğu tarih olarak görülmüştür. Yemlik kolzanın arıcılık faaliyeti açısından özellikle erken ilkbahar döneminde kolonilerde oluşan nektar ve polen yetersizliğinin çözümüne yönelik ideal bir arı merası bitkisi olduğu, Bingöl ve benzer ekolojik koşullara sahip bölgelerde 26 Nisan tarihi geçirilmeden bu bitkinin arıcılık açısından değerlendirilebileceği sonucuna varılmıştır.In this study, the effects of different sowing norms of Forage Rape on some yield characteristics, the mutual interactions between honey bees and the evaluation of forage rape as an alternative plant in the creation of bee pastures were carried out. Sowing norms of 400, 800, 1200, 1600, 2000 and 2400 g/da were used in the study. Data was taken twice a week. The flowering of the plant and the number of honeybees per m2, the duration of the honeybee in bloom, the plant height, the number of plants per m2, the number of flowers per plant, the number of flowers per m2, the number of side branches, capsule per plant, seed in capsule, seed properties such as yield and thousand grain weight were discussed. This research observed that, 1200-1600 g/da sowing norm produces the highest yield. April 26th was observed as the date when bees visited the Forage Rape the most, reached the highest height of the plant, and had the most flowers per plant and per m2. It has been concluded that Forage Rape is an ideal bee pasture plant for the solving nectar and pollen deficiency in beekeeping, especially in early spring periods in terms of beekeeping activity. İt is recommended that this plant should be further evaluated for beekeeping on dates prior to April 26th in Bingöl and regions with similar ecological conditions

Overview of the CLEF-2022 CheckThat! Lab Task 1 on Identifying Relevant Claims in Tweets

We present an overview of CheckThat! lab 2022 Task 1, part of the 2022 Conference and Labs of the Evaluation Forum (CLEF). Task 1 asked to predict which posts in a Twitter stream are worth fact-checking, focusing on COVID-19 and politics in six languages: Arabic, Bulgarian, Dutch, English, Spanish, and Turkish. A total of 19 teams participated and most submissions managed to achieve sizable improvements over the baselines using Transformer-based models such as BERT and GPT-3. Across the four subtasks, approaches that targetted multiple languages (be it individually or in conjunction, in general obtained the best performance. We describe the dataset and the task setup, including the evaluation settings, and we give a brief overview of the participating systems. As usual in the CheckThat! lab, we release to the research community all datasets from the lab as well as the evaluation scripts, which should enable further research on finding relevant tweets that can help different stakeholders such as fact-checkers, journalists, and policymakers

Overview of the CLEF–2022 CheckThat! Lab on Fighting the COVID-19 Infodemic and Fake News Detection

We describe the fifth edition of the CheckThat! lab, part of the 2022 Conference and Labs of the Evaluation Forum (CLEF). The lab evaluates technology supporting tasks related to factuality in multiple languages: Arabic, Bulgarian, Dutch, English, German, Spanish, and Turkish. Task 1 asks to identify relevant claims in tweets in terms of check-worthiness, verifiability, harmfullness, and attention-worthiness. Task 2 asks to detect previously fact-checked claims that could be relevant to fact-check a new claim. It targets both tweets and political debates/speeches. Task 3 asks to predict the veracity of the main claim in a news article. CheckThat! was the most popular lab at CLEF-2022 in terms of team registrations: 137 teams. More than one-third (37%) of them actually participated: 18, 7, and 26 teams submitted 210, 37, and 126 official runs for tasks 1, 2, and 3, respectively.</p