Poly(lactic acid) nanofibers containing phosphorylcholine grafts for transdermal drug delivery systems

© 2022 Elsevier LtdThe continuous and prolonged releases of chemotherapeutic drugs are required for successful treatment in cancer treatment. The project focused on a new material design to meet this requirement. We developed a constant and sustained release system and investigated the release profiles of Paclitaxel (PTX). Polylactic acid (PLA) nanofiber surface was grafted with poly (methacryloyloxyethyl phosphorylcholine) (PMPC) by the UV-induced grafting method. The morphological structure of the PLA nanofibers did not change with an increase in the MPC content. PMPC blocks contribute to the solubility of PTX, which shows low resolution. When the amount of MPC is 5%, the PTX loading efficiency increased two times compared with PLA nanofiber. The nanofiber mats exhibited an initial fast release during the first 3 h. Endothelial cells were cultured on nanofiber mats to investigate whether this material was toxic or not. The mats showed good biocompatibility with HUVEC. Thus, it was confirmed that nanofiber mats would not be toxic when releasing drugs during in vivo use. We think that PMPC facilitates the pass of drugs through the lipid-rich biological membrane and so anticancer drugs can be delivered to direct tumor sites

Part 2: biocompatibility evaluation of hydroxyapatite-based clinoptilolite and Al2O3 composites

The biocompatibility of clinoptilolite/alumina/bovine hydroxyapatite (Cp - Al2O3 - BHA) composite, at different ratio obtained by powder pressing process, were investigated studying the behavior of osteosarcoma (SAOS-2) cells. The biocompatibility was examined by means of cytotoxicity and cytocompatibility tests. The structure and morphology of bioceramic composites were studied by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR) technique. The results showed that these materials have no toxic effects. The natural composite that fabricated in this study may be a promising approach for bone engineering applications

Preparation of biocompatible, UV-cured fumarated poly(ether-ester)-based tissue-engineering hydrogels

The aim of this study was to develop biodegradable, photo-polymerizable in situ gel-forming systems prepared from a fumaric acid monoethyl ester (FAME) modified poly(lactide-co-glycolide) (PLGA) copolymer. By reacting lactide and glycolide in the presence of stannous octoate as a catalyst and 2-ethyl, 2-hydroxymethyl 1,3-propanediol as an initiator, hydroxyl terminated branched PLGA was synthesized. Afterwards, at room temperature hydroxyl terminated branched PLGA was reacted with fumaric acid monoethyl ester (FAME). N,N'-dicyclohexylcarbodiimide and triethylamine were used as a coupling agent and catalyst, respectively. The gel percentage, equilibrium mass swelling, degradation profile and polymerization kinetics of the hydrogels were investigated. All of the results were influenced by the amount of FAME modified PLGA co-polymer. Biocompatibility of the hydrogels was examined by using MTT cytotoxicity assay. According to the results, hydrogels are biocompatible and cell viability percentage depends on the amount of PLGA co-polymer. While the amount was 15% in hydrogel composition, cell viability was 100%, but after increasing the PLGA co-polymer amount to 30% the viability reduced to 78%. (C) Koninklijke Brill NV, Leiden, 201

Preparation of collagen modified photopolymers: A new type of biodegradable gel for cell growth

In this study a new branched methacrylated poly(propylene glycol-co-lactic acid) (PPG-PLA-IEM) and methacrylated cellulose acetate butyrate resin (CAB-IEM) were synthesized. Hydrogels with various amounts of PPG-PLA-IEM and CAB-IEM (25, 50 and 75 wt% IEM modified) were prepared by photopolymerization. Collagen tethered PEG-monoacrylate (PEGMA-collagen) was prepared and introduced as a bioactive moiety to modify the hydrogel in order to enhance cell affinity. In vitro attachment and growth of 3T3 mouse fibroblasts and human umbilical vein endothelial cells (HUVEC) on the hydrogels with and without collagen were also investigated. It was observed that, the collagen improves the cell adhesion onto the hydrogel surface. With the increasing amount of collagen, cell viability increased by 28% for ECV304 (P < 0.05) and 30% for 3T3 (P < 0.05)

Preparation of bow tie-type methacrylated poly(caprolactone-co-lactic acid) scaffolds: Effect of collagen modification on cell growth

A branched methacrylated poly(caprolactone-co-lactic acid) and methacrylated poly(tetramethylene ether glycol) (PTMG-IEM) resins were synthesized. 1H-NMR spectroscopy, attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) spectroscopy, and gel permeation chromatography confirmed the chemical structures of copolymers. The photoinitiated polymerization of formulation composed of various amounts of methacrylated poly(caprolactone-co-lactic acid), PTMG-IEM, poly(ethylene glycol) diacrylate, water, and photoinitiator were performed. The curing reactions were followed by photo-DSC (Differential scanning calorimetry). Gel fraction was calculated from the insoluble part and found as =93%. Swelling and contact angles were measured, and all increased with the increasing amount of PTMG-IEM in network formulations. In vitro degradation studies were performed at 37 degrees C in phosphate-buffered saline (pH 7.4). Collagen-modified polymers were also prepared and introduced as a bioactive moiety to modify the polymer to enhance cell affinity. To compare the cell adhesion affinity to the polymer with and without collagen, cell growth experiments were performed. The results showed that collagen improves the cell adhesion onto the polymer surface. With the increasing amount of collagen, cell viability increases 86% (ECV304, p?<?0.05) and 83% (3?T3, p?<?0.05). Copyright (C) 2011 John Wiley & Sons, Ltd

3D Printing of Gelatine/Alginate/β-Tricalcium Phosphate Composite Constructs for Bone Tissue Engineering

Bone tissue engineering studies have brought three-dimensional scaffolds into focus that can provide tissue regeneration with designed porosity and strengthened structure. Current research has concentrated on the fabrication of natural and synthetic polymer-based complex structures that closely mimic biological tissues due to their superior biocompatibility and biodegradabilities. Gelatine/Sodium Alginate hydrogels reinforced with different concentrations of beta-Tricalcium Phosphate (TCP) (10, 13, and 15 wt.%) were studied to form 3D bone tissue. Physical, mechanical, chemical, morphological properties and biodegradability of the constructs were investigated. Furthermore, in vitro biological assay with human osteosarcoma cell line (SAOS-2) was performed to determine the biocompatibility of the constructs. It is found that cell viability rates for all constructs were increased and maximum cell viability rate was attained for 20%Gelatine/2%Alginate/10%TCP (wt.). The present work demonstrates that 3D printed Gelatine/Alginate/TCP constructs with porous structures are potential candidates for bone tissue engineering applications

Developments of 3D polycaprolactone/beta-tricalcium phosphate/collagen scaffolds for hard tissue engineering

Şahin, Yeşim Müge (Arel Author)3D bioprinting provides an innovative strategy to fabricate a new composite scaffold material consisted in a porous and rough structure with using polycaprolactone (PCL), beta-tricalcium phosphate (?-TCP), and collagen as a building block for tissue engineering. We investigated the optimization of the scaffold properties based on the ?-TCP concentration using 3D bioprinting method. Computer-aided drawing was applied in order to digitally design the scaffolds while instead of solid filaments, materials were prepared as a blend solution and controlled evaporation of the solvent during the bioprinting was enabled the proper solidification of the scaffolds, and they were successfully produced with well-defined porous structure. This work demonstrated the feasibility of complex PCL/?-TCP/collagen scaffolds as an alternative in the 3D bioprinting engineering to the fabrication of porous scaffolds for tissue engineering. © 2019, Australian Ceramic Society.This study has been founded by BAPKO, Marmara University, grant no. FEN-C-YLP-090217-0066