Gene expression profile and injury sites in mice treated with Shiga toxin 2 and lipopolysaccharide as a Shiga toxin-associated hemolytic uremic syndrome

博士（医学）福島県立医科大

Enhanced production of nitric oxide, reactive oxygen species, and pro-inflammatory cytokines in very long chain saturated fatty acid-accumulated macrophages

<p>Abstract</p> <p>Background</p> <p>Deterioration of peroxisomal β-oxidation activity causes an accumulation of very long chain saturated fatty acids (VLCSFA) in various organs. We have recently reported that the levels of VLCSFA in the plasma and/or membranes of blood cells were significantly higher in patients with metabolic syndrome and in patients with coronary artery disease than the controls. The aim of the present study is to investigate the effect of VLCSFA accumulation on inflammatory and oxidative responses in VLCSFA-accumulated macrophages derived from X-linked adrenoleukodystrophy (X-ALD) protein (ALDP)-deficient mice.</p> <p>Results</p> <p>Elevated levels of VLCSFA were confirmed in macrophages from ALDP-deficient mice. The levels of nitric oxide (NO) production stimulated by lipopolysaccharide (LPS) and interferon-γ (IFN-γ), intracellular reactive oxygen species (ROS), and pro-inflammatory cytokines, including tumor necrosis factor-α (TNF-α), interluekin-6 (IL-6), and interleukin-12p70 (IL-12p70), were significantly higher in macrophages from ALDP-deficient mice than in those from wild-type mice. The inducible NO synthase (iNOS) mRNA expression also showed an increase in macrophages from ALDP-deficient mice.</p> <p>Conclusion</p> <p>These results suggested that VLCSFA accumulation in macrophages may contribute to the pathogenesis of inflammatory diseases through the enhancement of inflammatory and oxidative responses.</p

Development of Transfer Assist Robot Based on the User Needs

To develop technology based on the need for a Roboticbed® by the elderly and those with disabilities, this study extracts user needs and problems based on feasibility testing. This study makes it clear that those with severe disabilities want the Roboticbed® to support their independence in moving between bed and wheelchair and back. We propose a lifestyle for users and caregivers using the present version of the Roboticbed® based on experiments and extracted problems based on user needs. These results indicate that evaluation based on user needs is useful for specifying a design guide for user needs.首都大学東京学位論文甲第476号 副論文 (要約

Differences in response to treatment in children with severe IgA nephropathy according to patient age

Aim: To clarify whether the response to treatment of IgA nephropathy (IgAN) differs depending on patient age, we examined the response to treatment according to age of onset in children with IgAN. Methods: We collected data for 44 children with severe IgAN. The children were retrospectively divided into three groups based on their age at disease onset. Group 1 consisted of 24 children under 11 years old, group 2 consisted of 9 children aged 12 to 13 years, and group 3 consisted of 11 children aged over 14 years old. The clinical features and prognosis were analyzed for each group. Results: The urinary protein excretion and serum IgA values in group 3 were higher than those in groups 1 and 2 at the most recent follow up, and histological findings showed that the MESTCG scores in group 3 were higher than those in group 1. Furthermore, the incidence of patients with persistent nephropathy or renal insufficiency in group 3 was higher than those in groups 1 and 2. Conclusions: Patients aged 14 years and older with IgAN may respond poorly to treatment compared with those younger than 14 years old. Therefore, care must be taken regarding response to treatment and relapse when treating older children

使用者中心の福祉機器臨床評価手法 : プロトタイプロボティックベッドの評価

首都大学東京, 2015-03-25, 博士（作業療法学）, 甲第476号首都大学東

Regulation of the Rate of Dinucleation of a Monocopper(I) Complex Containing Bipyrimidine Rotary Units by Restricted Double Pyrimidine Rotation

New copper­(I) complexes with coordinated 2-(4′-methyl)­pyrimidinyl moieties were fabricated, and the isomerism of their pyrimidine ring linkage was investigated. The ligands bis­[2-(diphenylphosphino)­phenyl] ether (DPEPhos) and 4,4′-dimethyl-2,2′-bipyrimidine (dmbpm) were used to synthesize a heteroleptic copper­(I) complex, [Cu^I(DPEPhos)­(dmbpm)]·BF₄ (<b>1</b>·BF₄), and a dinuclear copper­(I) complex, [(Cu^I)₂(DPEPhos)₂(μ-dmbmp)]­(BF₄)₂ [<b>2</b>·(BF₄)₂]. The X-ray crystallographic structures, UV–vis absorption spectra, and luminescence properties of the complexes were analyzed. The thermodynamic and kinetic aspects of the isomerism of <b>1</b>·BF₄ were examined by variable-temperature NMR. Double pyrimidine ring rotation was found to be restricted sterically by the bulky DPEPhos ligands. This limited the number of the possible isomers: <b>1</b>·BF₄ showed only isomers with either one (<i>io</i> isomer) or both (<i>oo</i> isomer) of the two methyl groups positioned away from the copper center, while dinuclear <b>2</b>·(BF₄)₂ was only found as a symmetric (<i>io</i>–<i>io</i>) isomer, with each of the two methyl groups positioned toward different copper centers. The addition of [Cu­(MeCN)₂(DPEPhos)] (<b>3</b>·BF₄) allowed both isomers of <b>1</b>·BF₄ to form <b>2</b>·(BF₄)₂, although at different rates and via different pathways, which were analyzed using time-dependent UV–vis spectroscopy. The <i>io</i> isomer dinucleated more quickly than the <i>oo</i> isomer owing to it being able to form <b>2</b>·(BF₄)₂ (i) without bond dissociation and (ii) without a sterically congested <i>ii</i> configuration around the copper center. In contrast, <i>oo</i>-<b>1</b>·BF₄ required (i) recombination of the bipyrimidine coordination bonds or (ii) formation of a product with higher thermodynamic energy, unsymmetric (<i>ii</i>–<i>oo</i>) <b>2</b>·(BF₄)₂. These findings are interpreted as demonstrating a novel kinetic property: a conversion rate determined by pyrimidine ring inversion