Maternal outcome in preterm labor in a tertiary care hospital

Background: To study the fetal and maternal outcome in preterm labor cases.Methods: It was Prospective, single centered, observational study. All women with preterm labor in the study period i.e. from September 2018 to March 2020 were included in the study i.e. 100 patients.Results: Out of 100 subjects, majority of mothers 65(65%) were in age group of 21-30 years followed by 23 (23%) in age group of ≤20 years. Majority 52 (52%) of study subjects were primigravida followed by 28 (28%) having parity 1. Only one case had parity 4. Majority 36 (36%) of study subjects were in lower class followed by 28 (28%) cases in lower middle class. 23 (23%) cases in middle class and 6 (6%) were in upper class. Maximum 61 (61%) cases were unbooked cases and rest 39 (39%) cases were booked. Out of 90 babies who were <2500 grams, maximum 57 (63.33%) were low birth weight (less than 2.5 kg) followed by 22 (24.44%) being VLBW babies. 10 cases were ≥2.5 kg, which are not LBW babies according to WHO Classification of low birth weight. Majority 34 (47.22%) stayed for 1-10 days followed by 17 (23.61%) babies for 11-20 days. Also 15 (20.83%) babies stayed for 21-30 days.Conclusions: Compared with term infants, preterm infants are at high risk of overall morbidity and mortality. Clinical suspicion, early detection and correction of risk factors, institutional delivery and good neonatal care back up facilities can improve the outcome of preterm labour and decrease the maternal complications as well

The Objectifying Gaze: Impact of Sexualized Media on Viewer Gaze Behavior towards (Non)Traditional Attire

In this study, we investigate the priming effect of camera-induced objectifying-gaze, operationalised via sexualised music video(MV), on university students when looking at target female images in traditional (saree, salwar) and western (shirt-pant, short-dress) attires in the Indian demographics. We showed participants two videos where female leads wore unrevealing Indian attire (saree) and revealing western clothing in sexualised and traditional MVs respectively. We quantified gaze as fixation-duration and visit-count (revisit frequency) metrics. Our findings report that there is a priming effect of sexualised media. Female images in all attires are equally objectified and body-biased gaze is more pronounced in sexualised MV priming, short-dress is more objectified in traditional MV priming, and saree is less objectified overall, which confirms the social stereotypes on attire and respectability — traditionally clad (vs. non-traditional) women are seen as more moral and worthy of respect and protection compared to women in hypersexualized and objectified roles

Comparative analysis of fetomaternal outcome in antenatal women infected with pulmonary and extrapulmonary tuberculosis

Background: Active tuberculosis is a multiorgan disease caused by primary infection or as a re-activation of latent tuberculosis. The studies comparing maternal and fetal outcome in antenatal women with extrapulmonary tuberculosis and pulmonary tuberculosis is scanty therefore, in current study we outline and identify the demographic and clinical characteristics of pulmonary tuberculosis and extrapulmonary tuberculosis and comparing fetal and maternal outcome in pulmonary and extrapulmonary tuberculosis. The maternal complications and fetal complications of pulmonary tuberculosis were analysed compare with extra pulmonary tuberculosis.Methods: This was prospective observational study done in tertiary care centre over a period 1.5 year from January 2019 to June 2020. Institute ethical committee approval was obtained. Pregnant women in the age group of 18-42 years diagnosed with tuberculosis in antenatal period were included in our study for comparing fetal and maternal outcomes. They were evaluated for their symptom including clinical examination, microbiological, radiological and biochemical testing. We excluded those antenatal women with tuberculosis and adequately treated and cured and those women with tuberculosis who did not delivered during study period.Results: Most common complication associated with pulmonary tuberculosis was intrauterine growth restriction whereas in extrapulmonary preterm labour most common complication. Perinatal outcome worse in extrapulmonary tuberculosis as compared to pulmonary tuberculosis.Conclusions: Pregnancy with tuberculosis worsen the outcome of both mother and fetus. Our study depicted perinatal outcome is worse in Extrapulmonary TB than pulmonary TB. Atypical presentation of the Extrapulmonary TB may constitute diagnostic and therapeutic challenges thus a high clinical suspicion is needed

Establishment of Optogenetic Modulation of cAMP for Analyzing Growth, Biofilm Formation, and Virulence Pathways of Bacteria Using a Light-Gated Cyclase

In bacteria, cyclic adenosine monophosphate (cAMP) signaling plays an essential regulatory role whose modulation via optogenetic tools would provide researchers an immense opportunity to control biological processes simply by illumination. The cAMP signaling in bacteria is a complex network of regulatory pathways, which utilizes distinct proteomic resources under different nutrient environments. We established an optogenetic modulation of cAMP and studied important cellular process of growth, biofilm formation, and virulence in the model bacterium E. coli using a light-gated adenylate cyclase (LgAC) from Beggiatoa sp. Blue light-induced activation of LgAC elevated the cAMP level in a blue light-dependent manner in E. coli. Quantitative proteomics revealed a decrease in the level of certain proteins governing growth (PTS, Adk, AckA, GlnA, and EFP), biofilm formation (IhfA, flagellin, YajQ, YeaG, and HlfC), and virulence (ClpP, YebC, KatE, BtuE, and Zur) in E. coli cells expressing LgAC upon blue light illumination. This optogenetic modulation of cAMP would be useful for deciphering cAMP-associated host&ndash;pathogen signaling of bacterial systems. Proteome knowledge established by this research work would also be useful for the scientific community while adapting LgAC-based optogenetic modulation for studying other relevant cAMP-driven bacterial physiology (e.g., energy metabolism). The systematic utilization of the established method and more extensively designed experiments regarding bacterial growth, biofilm, survival, and virulence might provide a road map for the identification of new targets for developing novel antibacterial drugs

Laparoscopic treatment of annular pancreas in a neonate

Introduction: Duodenal obstruction due to annular pancreas is a rare entity. Among the causes of neonatal obstruction described by Robert Gross, annular pancreas comprises less than 1% of total group (Gross and Chisholm, 1944) [1]. Herewith, we present a case of annular pancreas causing duodenal obstruction in a neonate for which we performed laparoscopic duodeno-jejunostomy and placed a trans anastomotic naso-jejunal feeding tube. Technique description: A 2-day old neonate presented with complaints of bilious vomiting since birth. Clinical findings and radiological imaging were suggestive of duodenal obstruction. The patient was planned for diagnostic laparoscopy. Intraoperatively, a large annular pancreas was noted to obstruct the second part of duodenum. Duodeno-jejunal anastomosis was performed using polydioxanone 5-0 in a single layer. After completion of the posterior layer, a 6Fr infant feeding tube was placed trans anastomotic reaching mid jejunum. The patient tolerated the procedure well and feeds were started on POD 2 via the naso-jejunal trans-anastomotic tube. Conclusion: Laparoscopic duodeno-jejunostomy with the placement of a trans-anastomotic tube is beneficial for the patient as feeds can be started within 48 hours or even earlier. Moreover, laparoscopic approach increases precision in dissection due to greater magnification, decreases overall morbidity and improves cosmesis. Laparoscopic duodeno-jejunostomy, though technically challenging, is feasible in the neonatal age group

Collinsella aerofaciens linked with increased ethanol production and liver inflammation contribute to the pathophysiology of NAFLD

Summary: Non-alcoholic fatty liver disease (NAFLD) is an emerging global health problem and a potential risk factor for metabolic diseases. The bidirectional interactions between liver and gut made dysbiotic gut microbiome one of the key risk factors for NAFLD. In this study, we reported an increased abundance of Collinsella aerofaciens in the gut of obese and NASH patients living in India. We isolated C. aerofaciens from the fecal samples of biopsy-proven NASH patients and observed that their genome is enriched with carbohydrate metabolism, fatty acid biosynthesis, and pro-inflammatory functions and have the potency to increase ethanol level in blood. An animal study indicated that mice supplemented with C. aerofaciens had increased levels of circulatory ethanol, high levels of hepatic hydroxyproline, triglyceride, and inflammation in the liver. The present findings indicate that perturbation in the gut microbiome composition is a key risk factor for NAFLD

Additional file 1 of Recommendations for diabetic macular edema management by retina specialists and large language model-based artificial intelligence platforms

Supplementary Material

Table_9_Complementary crosstalk between palmitoylation and phosphorylation events in MTIP regulates its role during Plasmodium falciparum invasion.xlsx

Post-translational modifications (PTMs) including phosphorylation and palmitoylation have emerged as crucial biomolecular events that govern many cellular processes including functioning of motility- and invasion-associated proteins during Plasmodium falciparum invasion. However, no study has ever focused on understanding the possibility of a crosstalk between these two molecular events and its direct impact on preinvasion- and invasion-associated protein–protein interaction (PPI) network-based molecular machinery. Here, we used an integrated in silico analysis to enrich two different catalogues of proteins: (i) the first group defines the cumulative pool of phosphorylated and palmitoylated proteins, and (ii) the second group represents a common set of proteins predicted to have both phosphorylation and palmitoylation. Subsequent PPI analysis identified an important protein cluster comprising myosin A tail interacting protein (MTIP) as one of the hub proteins of the glideosome motor complex in P. falciparum, predicted to have dual modification with the possibility of a crosstalk between the same. Our findings suggested that blocking palmitoylation led to reduced phosphorylation and blocking phosphorylation led to abrogated palmitoylation of MTIP. As a result of the crosstalk between these biomolecular events, MTIP’s interaction with myosin A was found to be abrogated. Next, the crosstalk between phosphorylation and palmitoylation was confirmed at a global proteome level by click chemistry and the phenotypic effect of this crosstalk was observed via synergistic inhibition in P. falciparum invasion using checkerboard assay and isobologram method. Overall, our findings revealed, for the first time, an interdependence between two PTM types, their possible crosstalk, and its direct impact on MTIP-mediated invasion via glideosome assembly protein myosin A in P. falciparum. These insights can be exploited for futuristic drug discovery platforms targeting parasite molecular machinery for developing novel antimalarial therapeutics.</p

Table_1_Complementary crosstalk between palmitoylation and phosphorylation events in MTIP regulates its role during Plasmodium falciparum invasion.xlsx

Post-translational modifications (PTMs) including phosphorylation and palmitoylation have emerged as crucial biomolecular events that govern many cellular processes including functioning of motility- and invasion-associated proteins during Plasmodium falciparum invasion. However, no study has ever focused on understanding the possibility of a crosstalk between these two molecular events and its direct impact on preinvasion- and invasion-associated protein–protein interaction (PPI) network-based molecular machinery. Here, we used an integrated in silico analysis to enrich two different catalogues of proteins: (i) the first group defines the cumulative pool of phosphorylated and palmitoylated proteins, and (ii) the second group represents a common set of proteins predicted to have both phosphorylation and palmitoylation. Subsequent PPI analysis identified an important protein cluster comprising myosin A tail interacting protein (MTIP) as one of the hub proteins of the glideosome motor complex in P. falciparum, predicted to have dual modification with the possibility of a crosstalk between the same. Our findings suggested that blocking palmitoylation led to reduced phosphorylation and blocking phosphorylation led to abrogated palmitoylation of MTIP. As a result of the crosstalk between these biomolecular events, MTIP’s interaction with myosin A was found to be abrogated. Next, the crosstalk between phosphorylation and palmitoylation was confirmed at a global proteome level by click chemistry and the phenotypic effect of this crosstalk was observed via synergistic inhibition in P. falciparum invasion using checkerboard assay and isobologram method. Overall, our findings revealed, for the first time, an interdependence between two PTM types, their possible crosstalk, and its direct impact on MTIP-mediated invasion via glideosome assembly protein myosin A in P. falciparum. These insights can be exploited for futuristic drug discovery platforms targeting parasite molecular machinery for developing novel antimalarial therapeutics.</p

Table_8_Complementary crosstalk between palmitoylation and phosphorylation events in MTIP regulates its role during Plasmodium falciparum invasion.xlsx

Post-translational modifications (PTMs) including phosphorylation and palmitoylation have emerged as crucial biomolecular events that govern many cellular processes including functioning of motility- and invasion-associated proteins during Plasmodium falciparum invasion. However, no study has ever focused on understanding the possibility of a crosstalk between these two molecular events and its direct impact on preinvasion- and invasion-associated protein–protein interaction (PPI) network-based molecular machinery. Here, we used an integrated in silico analysis to enrich two different catalogues of proteins: (i) the first group defines the cumulative pool of phosphorylated and palmitoylated proteins, and (ii) the second group represents a common set of proteins predicted to have both phosphorylation and palmitoylation. Subsequent PPI analysis identified an important protein cluster comprising myosin A tail interacting protein (MTIP) as one of the hub proteins of the glideosome motor complex in P. falciparum, predicted to have dual modification with the possibility of a crosstalk between the same. Our findings suggested that blocking palmitoylation led to reduced phosphorylation and blocking phosphorylation led to abrogated palmitoylation of MTIP. As a result of the crosstalk between these biomolecular events, MTIP’s interaction with myosin A was found to be abrogated. Next, the crosstalk between phosphorylation and palmitoylation was confirmed at a global proteome level by click chemistry and the phenotypic effect of this crosstalk was observed via synergistic inhibition in P. falciparum invasion using checkerboard assay and isobologram method. Overall, our findings revealed, for the first time, an interdependence between two PTM types, their possible crosstalk, and its direct impact on MTIP-mediated invasion via glideosome assembly protein myosin A in P. falciparum. These insights can be exploited for futuristic drug discovery platforms targeting parasite molecular machinery for developing novel antimalarial therapeutics.</p