Uniform Micro-Patterning of an Arbitrary Surface

According to the literature, creating specific micro-level patterns on some surfaces can significantly reduce friction. To this effect, a method is presented to create a regular pattern of micro-level indentations on any irregular surface. Creating a uniform pattern on a regular surface is possible using commercial CAD software, where regular surface is the surface obtained by extrusion or revolution of a 2D sketch along any curve. But, it is complicated and often incorrect for irregular surfaces. The thesis presents the approach followed to create parameterized regular patterns on arbitrary surfaces. Three different algorithms are presented, each achieving a progressively increased quality solution. The last and best method provides a set of points with their corresponding normals to the surface to enable the creation of the patterning feature. The algorithm reads an STL file, a format neutral output of any CAD software and implements the method on the approximated surface. Each facet surface upon which the pattern has to be created is sliced by planes at specific distances from each other. The intersections of the facets and the planes are calculated and chains are formed from the intersections in each plane. Points are interpolated at the required pitch in different chains formed at the intersection of a single plane and the facets. This procedure is repeated for each plane. Thus, a pattern of points of specified pitch distance that can be as low as microns can be generated. Given specifications of a machine, this method generates the X, Y, and Z translations and the axis rotation angles needed to generate a g-code specific to a micro-milling machine. This code can be used directly for any metal removing process that has to create micro-level indentations on an arbitrary surface. If instead, the features are protrusions on some irregular surface, then the resultant points obtained with the developed approach can be used to apply the pattern at each of the identified locations

Study on awareness, perceptions, experiences in decision process of hysterectomy in women presenting to Goa Medical College

Background: Hysterectomy (removal of uterus) has many indications like fibroids, dysfunctional uterine bleeding, uterine prolapse and chronic pelvic pain. The purpose of this study is to know the level of awareness of women about hysterectomy, to study their experiences, to assess their involvement in decision making process and to develop a suitable protocol, counselling and education material to ensure women’s involvement.Methods: This is a cross sectional study using validated semi-structured interview questionnaire over 6 months (1st December, 2018 to 31st May, 2019) in Goa Medical College. Data was entered in epidata manager and analysed using SPSS 22 version. Categorical variables were expressed in percentages and proportions and quantitative variables in mean±SD. Significance was checked using Chi square test and association between dependent and independent variables was assessed by using bivariate analysis. P value <0.05 was taken as significant.Results: A total of 149 women were recruited into the study who were admitted for hysterectomy. The mean age of women was 49.3 years. The awareness with respect to various aspects of hysterectomy was good in 23.4% subjects and poor in 76.6% subjects. The awareness was significantly related to level of education (p=0.002), urban location (p=0.016) and socioeconomic status (p=0.028). Education was significant factor in decision-making process (p< 0.05). It was observed that good experience had positive correlation to their perception about hysterectomy.Conclusions: It was concluded that awareness about hysterectomy was significantly related to education, urban location and socio-economic status of women. Pre-operative education would help to reduce the post-operative symptoms for a women undergoing hysterectomy

Recombinant rubella vectors elicit SIV Gag-specific T cell responses with cytotoxic potential in rhesus macaques

AbstractLive-attenuated rubella vaccine strain RA27/3 has been demonstrated to be safe and immunogenic in millions of children. The vaccine strain was used to insert SIV gag sequences and the resulting rubella vectors were tested in rhesus macaques alone and together with SIV gag DNA in different vaccine prime-boost combinations. We previously reported that such rubella vectors induce robust and durable SIV-specific humoral immune responses in macaques. Here, we report that recombinant rubella vectors elicit robust de novo SIV-specific cellular immune responses detectable for >10 months even after a single vaccination. The antigen-specific responses induced by the rubella vector include central and effector memory CD4+ and CD8+ T cells with cytotoxic potential. Rubella vectors can be administered repeatedly even after vaccination with the rubella vaccine strain RA27/3. Vaccine regimens including rubella vector and SIV gag DNA in different prime-boost combinations resulted in robust long-lasting cellular responses with significant increase of cellular responses upon boost. Rubella vectors provide a potent platform for inducing HIV-specific immunity that can be combined with DNA in a prime-boost regimen to elicit durable cellular immunity