62 research outputs found
Streptococcus anginosus lung infection and empyema: A case report and review of the literature
Streptococcus milleri group (SMG) also referred to as the Streptococcus anginosus group. These are Gram-positive, variable hemolysis, catalase negative, microaerophilic, non-motile facultative anaerobes which have been known to cause abscesses in humans. We report a case of empyema caused by Streptococcus anginosus in a patient with an unresolved pneumonia for over a month. In early October 2018, the patient presented to an emergency room with the complaints of shortness of air, productive cough, chills, subjective fever and weight loss for 4 weeks. A chest X-ray revealed a left lower lobe pneumonia. He was treated with 250 mg of azithromycin for 4 days. During a follow-up visit in November 2018, he reported having persistent symptoms. The chest CT revealed a localized pleural fluid collection at the left lower chest highly suggestive of empyema. He was prescribed 100mg of doxycycline for a month. However, he was admitted to the hospital a week later due to worsening symptoms. The microbiological cultures for sputum and blood were negative; however, pleural fluid cultures grew Streptococcus anginosus resistant to clindamycin and erythromycin. The patient was treated with broad spectrum antimicrobial regimen in conjunction with surgical management. Initially, the patient underwent CT guided placement of chest tube with instillation of Tissue Plasminogen Activator (TPA) for the drainage of pleural fluid followed Video-assisted Thoracoscopy with lateral decortication and drainage of empyema of the left lung due to persistence of complicated effusion. There was remarkable improvement in his symptoms, and he recovered subsequently. Our case highlights the infections caused by the Streptococcus milleri group (SMG) in individuals with an unresolved pneumonia. Such patients should be diagnosed accurately and treated aggressively with rapid and effective interventions
Waterlogging tolerance in black gram [Vigna mungo (L.) Hepper] is associated with chlorophyll content and membrane integrity
Black gram (Vigna mungo L.) is waterlogging sensitive legume crop. We studied the effect of waterlogging stress on membrane stability index (MSI), lipid peroxidation, superoxide dismutase (SOD) activity, chlorophyll content and chlorophyll fluorescence in four Vigna genotypes namely (Uttara, T-44, IC530491, IC519330). Stress was imposed for 10 days at vegetative stage (30 days after sowing). Thereafter, excess water was drained to allow recovery in stressed plants. Waterlogging treatment significantly increased lipid peroxidation and SOD activity in all the genotypes, which showed the oxidative injury posed by stress conditions. Chlorophyll content and fluorescence reduced under stress conditions. SOD activity, MSI and chlorophyll content was more in IC530491 and IC519330, T44 as compared to Uttara. Lipid peroxidation was high in Uttara. Though chlorophyll fluorescence reduced in all the genotypes under waterlogging, genotypic differences were non-significant. More efficient antioxidative scavenging to maintain membrane stability and chlorophyll content in black gram was found to be associated with tolerance to waterlogging
Legume Genetic Resources: Status and Opportunities for Sustainability
Legumes are one of the most valuable gifts of nature to man, animal, and environment. These are sustainable, affordable, water-efficient, and low-carbon footprint crop. Globally, the share of grain legume accessions is 15% of 7.4 million accessions conserved in genebanks, of which more than half of germplasm in genebanks are without characterization and evaluation data which ultimately limit the utilization of germplasm in legume improvement programs. Characterization of all genebank accessions should be of utmost priority for enhancing the utilization. The development of core, mini-core, reference sets, and trait-specific germplasm has provided route to crop breeders for mining genebanks. Identification of new sources of variation became easy with these subsets, but the entire collection also needs to be evaluated for unique and rare traits. In crop species with narrow genetic base, utilization of crop wild relatives as well as new resources aids to widen the genetic base of legume cultigens
Electrical Discharge Coating a Potential Surface Engineering Technique: A State of the Art
Electrical discharge coating (EDC) process is used to deposit material on workpiece surface from sacrificial or green compact tool electrode in an electrical discharge machine. The paper presents the mechanism of EDC using green compact electrode and powder mixed dielectric methods. The tool electrode material, electrode size, process parameters, and type of dielectrics can directly affect the surface integrity of workpiece. Here, a process map of EDC as a function of process parameters, its classification, advantages, and applications for a wide range of engineering materials offers a proper template for the evaluation of coating phenomena. This study shows that EDC is an economic process as compared to other costlier techniques. Additionally, the effect of various EDM and EDC parameters on surface integrity and tribological behavior of deposited coatings is studied with their pros and cons. Finally, the current research trends of EDC and its challenges are elaborated.info:eu-repo/semantics/publishedVersio
Minni Payaru [Vigna stipulacea (Lam.) Kuntz.]: an underutilized ancient legume of India
Minni Payaru [Vigna stipulacea (Lam.) Kuntz] is an underutilized traditional legume species mainly used for food, animal fodder and green manure, particularly in Southern India. In the era of global climate change, this indigenous crop has the ability to survive under harsh environment and also has resistance against various biotic and abiotic stresses. Based on acquired knowledge about this crop, farmers prefer Minni Payaru compared to other commercial kharif pulses. Though Minni Payaru has huge potential to be used as main legume crop, it has not gained popularity in India. Currently, the cultivation of Minni Payaru is restricted to the Southern regions of India. The present review summarises the budding role of Minni Payaru in human nutrition, animal fodder and green manure
Identification of novel resistant sources for ascochyta blight (Ascochyta rabiei) in chickpea
Chickpea (Cicer arietinum L.) is the second largest pulse crop grown worldwide and ascochyta blight caused by Ascochyta rabiei (Pass.) Labr. is the most devastating disease of the crop in all chickpea growing areas across the continents. The pathogen A. rabiei is highly variable. The resistant sources available are not sufficient and new sources needs to be identified from time to time as resistance breakdown in existing chickpea varieties is very frequent due to fast evolution of new pathotypes of the pathogen. Therefore, this work was undertaken to evaluate the existing chickpea germplasm diversity conserved in Indian National Genebank against the disease under artificial epiphytotic conditions. An artificial standard inoculation procedure was followed for uniform spread of the pathogen. During the last five winter seasons from 2014â15 to 2018â19, a total of 1,970 accessions
have been screened against the disease and promising accessions were identified and validated. Screening has resulted in identification of some promising chickpea accessions such as IC275447, IC117744, EC267301, IC248147 and EC220109 which have shown the disease resistance (disease severity score ïżœ3) in multiple seasons and locations. Promising accessions can serve as the potential donors in chickpea improvement programs. The frequency of resistant and moderately resistant type accessions was comparatively higher in accessions originated from Southwest Asian countries particularly Iran and Syria than the accessions originated from Indian sub-continent. Further large scale screening of chickpea germplasm originated from Southwest Asia may result in identifying new resistant sources for the disease
Response of wheat (Triticum aestivum) landraces from north-western Himalaya to rice weevil (Sitophilus oryzae)
Wheat (Triticum aestivum L.) landraces (one hundred) from the north-western Himalayan region were evaluated for their response to rice weevil, Sitophilus oryzae L. under no-choice artificial infestation conditions in the laboratory. The differential reaction was based on the parameters, viz. number of adults emerged, development period, growth index (GI) and weight loss in seeds. On the basis of GI, these landraces were categorised as resistant (six landraces with GI from 0.33-0.49), moderately resistant (13 landraces, GI from 0.52-0.97), moderately susceptible (41 landraces, GI ranging from 1.01-1.50), and susceptible (41 with GI from 1.51-1.86). On the basis of seed weight loss, eight landraces were found resistant (8.3-18.5%), 32 as moderately resistant (20.1-39.5), 51 as moderately susceptible (40.3-59.9), and 10 landraces (61.4-68.8) were found susceptible. Four landraces, viz. IC393109, IC392578, IC444217 and IC589276 were found resistant both on the basis of GI and seed weight loss. These landraces can be used in breeding programme for development of wheat cultivars resistant to S. oryzae
Khesari (Lathyrus sativus L.), an ancient legume for future gain: An expedition collection from parts of West Bengal state of Eastern India
395-403Grasspea is one of the staple foods of the local people living in the eastern parts of India. An expedition was undertaken during March, 2020 to collect germplasm of grasspea in the lower-Gangetic riverine belt and coastal areas of West Bengal of eastern India lying between latitude 21.43-24.44°N and longitude 87.23-88.90°E. From the results of a structured questionnaire administered to grasspea farmers in 57 villages located in 96 local government areas, it appeared that grasspea is the primary winter pulse cultivated in this region. Large variability of germplasm exist, ranging from small to bold seed, early to late maturing types, moderate to the high biomass type of grasspea. Most of these landraces have been adopted over the years from neighbouring communities, but in a few instances, the varietal replacement was noted, which came either through the involvement of government departments or local seed dealers. The highest proportion of the accessions (52.38%) was collected from the Purba Medinipur district, and the lowest (19.05%) was from Paschim Medinipur. On-spot evaluation of morphological traits, variations was detected in the descriptor characteristics across the locations. A total of 21 accessions was collected and assessed on-spot for different characters, viz., the seed's size, shape, seed colour, taste and texture revealed significant variation. The implications of this survey results for grasspea improvement in India are discussed in the present study
Evaluation and Multivariate Analysis of Cowpea [Vigna unguiculata (L.) Walp] Germplasm for Selected NutrientsâMining for Nutri-Dense Accessions
A total of 120 highly diverse cowpea [Vigna unguiculata (L.) Walp] genotypes, including indigenous and exotic lines, were evaluated for different biochemical traits using AOAC official methods of analysis and other standard methods. The results exhibited wide variability in the content of proteins (ranging from 19.4 to 27.9%), starch (from 27.5 to 42.7 g 100 gâ1), amylose (from 9.65 to 21.7 g 100 gâ1), TDF (from 13.7 to 21.1 g 100 gâ1), and TSS (from 1.30 to 8.73 g 100 gâ1). The concentration of anti-nutritional compounds like phenols and phytic acid ranged from 0.026 to 0.832 g 100 gâ1 and 0.690 to 1.88 g 100 gâ1, respectively. The correlation coefficient between the traits was calculated to understand the inter-trait relationship. Multivariate analysis (PCA and HCA) was performed to identify the major traits contributing to variability and group accessions with a similar profile. The first three principal components, i.e., PC1, PC2, and PC3, contributed to 62.7% of the variation, where maximum loadings were from starch, followed by protein, phytic acid, and dietary fiber. HCA formed six distinct clusters at a squared Euclidean distance of 5. Accessions in cluster I had high TDF and low TSS content, while cluster II was characterized by low amylose content. Accessions in cluster III had high starch, low protein, and phytic acid, whereas accessions in cluster IV contained high TSS, phenol, and low phytic acid. Cluster V was characterized by high protein, phytic acid, TSS, and phenol content and low starch content, and cluster VI had a high amount of amylose and low phenol content. Some nutri-dense accessions were identified from the above-mentioned clusters, such as EC169879 and IC201086 with high protein (>27%), TSS, amylose, and TDF content. These compositions are promising to provide practical support for developing high-value food and feed varieties using effective breeding strategies with a higher economic value
Corrigendum: Evaluation and multivariate analysis of cowpea [Vigna unguiculata (L.) walp] germplasm for selected nutrientsâmining for nutri-dense accessions
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