26 research outputs found
Photo-oxidative action in cervix carcinoma cells induced by HpD — mediated photodynamic therapy
Photodynamic therapy leads to oxidative stress through the generation of free radicals. Oxidative stress causes damage to cellular macromolecules such as nucleic acids, proteins and lipids. Aim: To examine the hematoporphyrin derivative (HpD) — mediated photodynamic effect on cervical adenocarcinoma cell line HeLa. Methods: The HpD localization in HeLa cells was analyzed by confocal microscopy with epi-fluorescence system. Lipid peroxidation (LPO) was estimated by measurement of the concentration of malondialdehyde, protein degradation — by modified Ellman’s method, superoxide dysmutase (SOD) — using Ransod Kit. The expression of inducible nitric oxide synthase (iNOS) was detected by immunocytochemical staining. Results: The HpD was distributed all over the cytoplasm with preferential localization in the inner side of the plasma membrane and around the nuclear envelope. The process of photosensitizer distribution was time dependent. PDT-HpD increased the level of malonodialdehyde (MDA), SOD activity and the expression of iNOS in HeLa cells. However, PDT induced the decrease in the level of protein-associated thiol groups. Conclusions: Our study showed the important role of PDT-mediated oxidative stress in HeLa cells. HpD-PDT might be alternative and less invasive approach for treatment of patients with cervical cancer resistant for standard chemotherapy and radiotherapy
Maternal Lipids as Strong Determinants of Fetal Environment and Growth in Pregnancies With Gestational Diabetes Mellitus
OBJECTIVE—To determine the contribution of maternal glucose and lipids to intrauterine metabolic environment and fetal growth in pregnancies with gestational diabetes mellitus (GDM)
The estimation of oxidative stress markers and apoptosis in right atrium auricles cardiomyocytes of patients undergoing surgical heart revascularisation with the use of warm blood cardioplegia.
A simple and established method of tissue culture of human gingival fibroblasts for gingival augmentation.
Assessing the impact of low level laser therapy (LLLT) on biological systems: a review
PURPOSE: Low level laser therapy (LLLT) in the visible to near infrared spectral band (390-1100 nm) is absorption of laser light at the electronic level, without generation of heat. It may be applied in a wide range of treatments including wound healing, inflammation and pain reduction. Despite its potential beneficial impacts, the use of lasers for therapeutic purposes still remains controversial in mainstream medicine. Whilst taking into account the physical characteristics of different qualities of lasers, this review aims to provide a comprehensive account of the current literature available in the field pertaining to their potential impact at cellular and molecular levels elucidating mechanistic interactions in different mammalian models. The review also aims to focus on the integral approach of the optimal characteristics of LLLT that suit a biological system target to produce the beneficial effect at the cellular and molecular levels. METHODS: Recent research articles were reviewed that explored the interaction of lasers (coherent sources) and LEDs (incoherent sources) at the molecular and cellular levels. RESULTS: It is envisaged that underlying mechanisms of beneficial impact of lasers to patients involves biological processes at the cellular and molecular levels. The biological impact or effects of LLLT at the cellular and molecular level could include cellular viability, proliferation rate, as well as DNA integrity and the repair of damaged DNA. This review summarizes the available information in the literature pertaining to cellular and molecular effects of lasers. CONCLUSIONS: It is suggested that a change in approach is required to understand how to exploit the potential therapeutic modality of lasers whilst minimizing its possible detrimental effects
Original Article Dynamic Oxidoreductive Potential of Astringent Retraction Agents (gingival margin retraction agents / cytotoxicity / human gingival fibroblasts)
Early Detection of Coronary Artery Diseases Using Endocrine Markers
© 2020, Springer Nature Switzerland AG. Cardiovascular diseases including coronary artery disease is the leading cause of death in the well developed and developing countries of the 21st century and has a higher rate of mortality and morbidity. Dysfunction of the pituitary, thyroid, and parathyroid glands caused cardio/cardiovascular diseases including changes in blood pressure, contractility of myocardium - systolic and diastolic myocardial functions, endothelial and dyslipidemia. Dysfunction of thyroid, parathyroid and adrenocorticotropic hormones caused imbalance of endocrine system such as hyper and hypo function, effects on pathophysiology of the cardiovascular system
Spasticity-reducing hand surgery for patients with upper motor neuron injuries: a prospective study of 30 patients with a one-year follow-up
The influence of photodynamic therapy on apoptosis in human melanoma cell line
Melanoma is the most severe of all skin cancers as it may grow rapidly and metastasize. The application of photodynamic therapy (PDT) opens new perspectives in treatment of this cancer. Numerous studies suggest that the exposure of tumor cells to PDT can lead to cell death <i>via</i> two separate processes: apoptosis or necrosis. The aim of this study was to assess <i>in vitro</i> photodynamic therapy which induces apoptosis in the human Beidegröm Melanoma (BM) cell line, using neutral comet assay. The cells were incubated with Photofrin II (15 &#956;g/ml and 30 &#956;g/ml) 4 h before and 3 h after irradiation for 5 or 10 min with the light intensity of 10 mW/cm<sup>2</sup>, using a lamp with red filter (632.8 nm). The percentage of apoptotic cells was significantly higher after PDT comparing to control cells. We observed 25% and 70% of apoptotic cells after shorter irradiation and treatment with 15 &#956;g/ml and 30 &#956;g/ml of Ph II, respectively. After longer irradiation, the respective values were 71.9% and 90%. The results suggest that induction of apoptosis is an important determinant of photodynamic sensitivity in the studied cell line and that some types of DNA damage are dependent on photosensitizer concentration and time of irradiation