Heterologous expression system comprising a b. subtilis promoter

The present invention relates to expression cassettes suitable for the expression of a heterologous gene. Furthermore, the invention relates to recombinant plasmids comprising such expression cassettes. Also, the invention relates to host cells comprising such cassettes or plasmids. Another embodiment relates to promoters for use in an expression cassette and in heterologous expression systems. Finally, an embodiment relates to methods for the expression of a heterologous gene.<br/

Export and modification of (poly)peptides in the lantibiotic way

The invention includes a method for harvesting a polypeptide produced by a host cell, wherein the polypeptide has not undergone intra-cellular post-translational modification, such as dehydration of a serine or a threonine, and/or thioether bridge formation. The invention also includes a method for producing thioether containing peptides and dehydroalanine/dehydrobutyrine-containing peptides, wherein extracellularly thioether rings may be formed