Інфокомунікаційні технології у вищій школі

У статті представлені результати розробки оптимальних з погляду дидактики технологічних підходів до розв’язання навчальних завдань вищої школи новітніми засобами ІКТ, а також оцінка ефективності їх застосування у порівнянні з традиційними методиками і засобами навчання

The fate of oxidative strand breaks in mitochondrial DNA

Mitochondrial DNA (mtDNA) is particularly vulnerable to somatic mutagenesis. Potential mechanisms include DNA polymerase γ (POLG) errors and the effects of mutagens, such as reactive oxygen species. Here, we studied the effects of transient hydrogen peroxide (H(2)O(2) pulse) on mtDNA integrity in cultured HEK 293 cells, applying Southern blotting, ultra-deep short-read and long-read sequencing. In wild-type cells, 30 min after the H(2)O(2) pulse, linear mtDNA fragments appear, representing double-strand breaks (DSB) with ends characterized by short GC stretches. Intact supercoiled mtDNA species reappear within 2–6 h after treatment and are almost completely recovered after 24 h. BrdU incorporation is lower in H(2)O(2)-treated cells compared to non-treated cells, suggesting that fast recovery is not associated with mtDNA replication, but is driven by rapid repair of single-strand breaks (SSBs) and degradation of DSB-generated linear fragments. Genetic inactivation of mtDNA degradation in exonuclease deficient POLG p.D274A mutant cells results in the persistence of linear mtDNA fragments with no impact on the repair of SSBs. In conclusion, our data highlight the interplay between the rapid processes of SSB repair and DSB degradation and the much slower mtDNA re-synthesis after oxidative damage, which has important implications for mtDNA quality control and the potential generation of somatic mtDNA deletions

Radiation-induced changes in infra-red spectrum of black zinc diphosphide monocrystals

For the first time the effect of ionizing particles of different type (gamma-quanta, electrons) and neutrons upon infra-red spectra of zinc diphosphide has been studied. It was shown that the defects of phosphorus sublattice, that are the principal centers of optical activity in the infra-red (IR), are responsible for structural evolution of zinc diphosphide crystals in the process interaction with radiation of different types

Formation of communicational and informational competence of future professionals of physical education and sport.

The technology of formation communication information competency of the future experts of physical training and sports is presented. 240 students of 4 rates of two institutes participated in research. Data of questionnaire of the future experts are used. Importance of use of new information technologies in educational process is exhibited. Milestones of introduction of computer and multimedia means are defined. Necessity of initiating for educational process of discipline «Sports - pedagogical computer science» is justified

Influence of structural defects on photoconductivity of zinc diphosphide

Spectral distribution of photoconductivity of initial and gamma-irradiated p-type zinc diphosphide crystals of tetragonal modification has been studied. It is supposed that band hn = 1.65 eV responses to clusters of defects existing in initial crystals. Complex defects appearing during high temperature treatment (400oC) form band hn = 1.55-2.14 eV. Irradiation leads to the decrease of dark current and appearing of acceptor type level which can be annealed in the temperature interval 20-100⁰C