61 research outputs found
Evaluation of antiplatelet activity of novel guanidine derivatives in the aspects of their adrenergic receptor activity
Designed acetamide derivatives based on guanidine and various heteroaryl carboxylic acids, were preliminary in vitro study of their adrenergic receptor affinity and anti-plateled effects. The obtained results have showed that exchange of 2,6-dichloro-phenyl substituent of guanidine into heteroaryl moieties, caused the decrease of receptor affinity, especially for α1-adrenoceptors. The observed receptor profile of activity for α2BAR was not changed compared to α1-ARs. Moreover, the observed effects on platelet aggregation induced by sub-threshold concentration of collagen and adrenaline strongly suggested that antiaggregant effect of N- (diaminomethylene)-2-(pyridin-3-yl)acetamide and N-(diaminomethylene)-2-(pyridin-4-yl)acetamide depends on their α2B-ARs antagonistic activity
Aryl-1,3,5-triazine ligands of histamine receptor attenuate inflammatory and nociceptive response to carrageen, zymosan and lipopolysaccharide
Objective and design Histamine receptor () offers a
great potential for new therapeutic strategies for the treatment
of inflammation-based diseases. The aim of this study
is to present the pharmacological profile of two recently
synthesized ligands of with particular reference to
their anti-inflammatory and analgesic activity.
Materials and subjects We used mice and rats in the
in vivo tests. We also used murine RAW 264.7 cells and
isolated guinea-pig ileum in in vitro test.
Treatments In the in vivo tests, animals were pre-treated
with the increasing doses of investigated compounds (12.5,
25 and 50 mg/kg) and reference compounds: JNJ7777120 (25 mg/kg), indomethacin (10 mg/kg). Macrophages were
pre-treated with two concentrations of tested compounds
100 and 10 M.
Methods We examined anti-inflammatory and analgesic
effects of the new antagonists in the in vivo models of
inflammation induced by carrageenan or zymosan. We
assessed the level of cAMP and release of cytokines, ROS
and NO in lipopolysaccharide (LPS)-stimulated RAW
264.7 macrophages. Moreover, we assessed the affinity of
the investigated compounds for histamine receptor in
functional studies. Results Both investigated compounds reduced paw edema,
mechanical and thermal hyperalgesia in the carrageenaninduced
acute inflammation. Moreover, administration of
the investigated compounds resulted in decreased granulocyte
influx and attenuated nociceptive reaction in the
zymosan-induced peritonitis model. In the same model of
inflammation, the investigated compounds reduced vascular
permeability; however, this effect was observed only
after the highest applied dose. Furthermore, the test compounds
had no impact on cell viability in the experiments
on RAW 264.7 macrophages. In these cells, stimulated
with LPS, the test compounds decreased reactive oxygen
species (ROS) production. They increased the cellular
concentration of cAMP and attenuated the production of
inflammatory cytokines such as and . All
results were comparable to those obtained for the reference
compound JNJ7777120 with the exception of the impact on
NO production. Nevertheless, this effect was similar to that
obtained for the other reference compound rolipram, which
is a phosphodiesterase 4 (PDE 4) inhibitor. Further
experiments revealed that both of the investigated compounds
possessed relatively low affinity for histamine H
receptor and do not inhibit the activity of the PDE 4B1
enzyme. In addition, all the effects of the investigated compounds in in vivo experiments were observed at doses
that did not cause neurologic deficits in rotarod test and did
not reduce spontaneous locomotor activity.
Conclusions Our results demonstrate the anti-inflammatory
and analgesic activity of the new aryl-1,3,5-triazine
derivatives, which are primarily -dependent
New spirohydantoin derivatives : synthesis, pharmacological evaluation and molecular modeling study
A series of new arylpiperazinylpropyl derivatives of 8/6-phenyl-1,3-diazaspiro[4.5]decan-2,4-dione and spiro[imidazolidine-4,1í-indene/naphthalene]-2,5-dione was synthesized and their affinity was evaluated toward serotonin 5-HT1A, 5-HT2A, 5-HT7 receptors, dopaminergic D2, D3 receptors, adrenergic α1 receptors, and serotonin transporter (SERT). The highest affinity for serotonin 5-HT1A/2A/7 receptors was found for compounds containing a tetralin or indane moiety in the imide part. Among these, two compounds (19, 20) were selected for further pharmacological in vivo studies. A binding mode of representative molecule 19, which behaved as a 5-HT1A agonist and weak 5-HT7 antagonist in the site of 5-HT1A/7, was also analyzed in computationa studies. Moreover, two highly selective (9 and 11) 5-HT2A receptor antagonists were obtained
Synthesis, computational and experimental pharmacological studies for (thio)ether-triazine 5-HT6R ligands with noticeable action on AChE/BChE and chalcogen-dependent intrinsic activity in search for new class of drugs against Alzheimer's disease
Alzheimer's disease is becoming a growing problem increasing at a tremendous rate. Serotonin 5-HT6 receptors appear to be a particularly attractive target from a therapeutic perspective, due to their involvement not only in cognitive processes, but also in depression and psychosis. In this work, we present the synthesis and broad biological characterization of a new series of 18 compounds with a unique 1,3,5-triazine backbone, as potent 5-HT6 receptor ligands. The main aim of this research is to compare the biological activity of the newly synthesized sulfur derivatives with their oxygen analogues and their N-demethylated O- and S-metabolites obtained for the first time. Most of the new triazines displayed high affinity (Ki < 200 nM) and selectivity towards 5-HT6R, with respect to 5-HT2AR, 5-HT7R, and D2R, in the radioligand binding assays. For selected, active compounds crystallographic studies, functional bioassays, and ADME-Tox profile in vitro were performed. The exciting novelty is that the sulfur derivatives exhibit an agonistic mode of action contrary to all other compounds obtained to date in this chemical class herein and previously reported. Advanced computational studies indicated that this intriguing functional shift might be caused by presence of chalcogen bonds formed only by the sulfur atom. In addition, the N-demethylated derivatives have emerged highly potent antioxidants and, moreover, show a significant improvement in metabolic stability compared to the parent structures. The cholinesterase study present micromolar inhibitory AChE and BChE activity for both 5-HT6 agonist 19 and potent antagonist 5. Finally, the behavioral experiments of compound 19 demonstrated its antidepressant-like properties and slight ability to improve cognitive deficits, without inducing memory impairments by itself. Described pharmacological properties of both compounds (5 and 19) allow to give a design clue for the development of multitarget compounds with 5-HT6 (both agonist and antagonist)/AChE and/or BChE mechanism in the group of 1,3,5-triazine derivatives
KM-408, a novel phenoxyalkyl derivative as a potential anticonvulsant and analgesic compound for the treatment of neuropathic pain
BACKGROUND: Epilepsy frequently coexists with neuropathic pain. Our approach is based on the search for active compounds with multitarget profiles beneficial in terms of potential side effects and on the implementation of screening for potential multidirectional central activity. METHODS: Compounds were synthesized by means of chemical synthesis. After antiseizure and neurotoxicity screening in vivo, KM-408 and its enantiomers were chosen for analgesic activity evaluations. Further safety studies included acute toxicity in mice, the effect on normal electrocardiogram and on blood pressure in rats, whole body plethysmography in rats, and in vitro and biochemical assays. Pharmacokinetics has been studied in rats after iv and po administration. Metabolism has been studied in vivo in rat serum and urine. Radioligand binding studies were performed as part of the mechanism of action investigation. RESULTS: Selected results for KM-408: K(i) sigma = 7.2*10(–8); K(i) 5-HT(1A) = 8.0*10(–7); ED(50) MES (mice, ip) = 13.3 mg/kg; formalin test (I phase, mice, ip)—active at 30 mg/kg; SNL (rats, ip)—active at 6 mg/kg; STZ-induced pain (mice, ip)—active at 1 mg/kg (von Frey) and 10 mg/kg (hot plate); hot plate test (mice, ip)—active at 30 mg/kg; ED(50) capsaicin test (mice, ip) = 18.99 mg/kg; tail immersion test (mice)—active at 0.5%; corneal anesthesia (guinea pigs)—active at 0.125%; infiltration anesthesia (guinea pigs)—active at 0.125%. CONCLUSIONS: Within the presented study a novel compound, R,S-2-((2-(2-chloro-6-methylphenoxy)ethyl)amino)butan-1-ol hydrochloride (KM-408) with dual antiseizure and analgesic activity has been developed for potential use in neuropathic pain treatment. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s43440-022-00431-7
Interwencja kryzysowa : konteksty indywidualne i społeczne
Praca recenzowana / Peer-reviewed paperOddajemy dziś w Twoje ręce, Czytelniku, monografię, która w zamyśle redaktorów
miała zostać poświęcona interwencji środowiskowej, tzw. nurtowi ekosystemu.
Zamiaru tego nie udało się jednak w pełni zrealizować, z uwagi na
stosunkowo niewielkie zainteresowanie polskich badaczy i praktyków teoretycznymi,
jak i praktycznymi problemami interwencji kryzysowej społeczności
i na rzecz społeczności, oraz jej specyfiką w polskich warunkach i realiach.
Brak zainteresowania wydaje się po części wynikać z małego zaangażowania
polskich służb pomocowych w nurty środowiskowej prewencji i działalności
pomocowej.
Ponad 20 lat rozwoju interwencji kryzysowej w naszym państwie zaowocowało
rozbudową instytucjonalnych struktur interwencyjnych, a także przygotowaniem
i kształceniem kadry interwentów kryzysowych, podejmujących
się pomocy w sytuacjach kryzysowych i traumatycznych, w ramach instytucjonalnych
powiązanych z pomocą społeczną, psychologiczną, rzadziej medyczną.
Prezentowane w naszej monografii opracowania autorskie w pewien
sposób odzwierciedlają problemy angażujące polskich interwentów kryzysu
zarówno w aspekcie teoretycznym, jak praktyki interwencyjnej. Zanim jednak
wprowadzimy Czytelników w problematykę poszczególnych opracowań
monografii, chcielibyśmy pokrótce scharakteryzować stany kryzysowe, będące
przedmiotem interwencyjnych oddziaływań ze współczesnej perspektywy
ekologiczno-systemowej.Publikacja powstała w ramach projektu badawczego Krakowskiej Akademii im. Andrzeja Frycza Modrzewskiego nr WPiNH/DS/3/2015-KO
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