10 research outputs found
Nanoscale Metal–Organic Frameworks for Real-Time Intracellular pH Sensing in Live Cells
Real-time measurement
of intracellular pH in live cells is of great
importance for understanding physiological/pathological processes
and developing intracellular drug delivery systems. We report here
the first use of nanoscale metal–organic frameworks (NMOFs)
for intracellular pH sensing in live cells. Fluorescein isothiocyanate
(FITC) was covalently conjugated to a UiO NMOF to afford F-UiO NMOFs
with exceptionally high FITC loadings, efficient fluorescence, and
excellent ratiometric pH-sensing properties. Upon rapid and efficient
endocytosis, F-UiO remained structurally intact inside endosomes.
Live cell imaging studies revealed endo- and exocytosis of F-UiO and
endosome acidification in real time. Fluorescently labeled NMOFs thus
represent a new class of nanosensors for intracellular pH sensing
and provide an excellent tool for studying NMOF–cell interactions
A Chlorin-Based Nanoscale Metal–Organic Framework for Photodynamic Therapy of Colon Cancers
We
report here the rational design of the first chlorin-based nanoscale
metal–organic framework (NMOF), DBC-UiO, with much improved
photophysical properties over the previously reported porphyrin-based
NMOF, DBP-UiO. Reduction of the DBP ligands in DBP-UiO to the DBC
ligands in DBC-UiO led to a 13 nm red shift and an 11-fold increase
in the extinction coefficient of the lowest-energy Q band. While inheriting
the crystallinity, stability, porosity, and nanoplate morphology of
DBP-UiO, DBC-UiO sensitizes more efficient <sup>1</sup>O<sub>2</sub> generation and exhibits significantly enhanced photodynamic therapy
(PDT) efficacy on two colon cancer mouse models as a result of its
improved photophysical properties. Both apoptosis and immunogenic
cell death contributed to killing of cancer cells in DBC-UiO-induced
PDT
Nanoscale Metal–Organic Frameworks for Real-Time Intracellular pH Sensing in Live Cells
Real-time measurement
of intracellular pH in live cells is of great
importance for understanding physiological/pathological processes
and developing intracellular drug delivery systems. We report here
the first use of nanoscale metal–organic frameworks (NMOFs)
for intracellular pH sensing in live cells. Fluorescein isothiocyanate
(FITC) was covalently conjugated to a UiO NMOF to afford F-UiO NMOFs
with exceptionally high FITC loadings, efficient fluorescence, and
excellent ratiometric pH-sensing properties. Upon rapid and efficient
endocytosis, F-UiO remained structurally intact inside endosomes.
Live cell imaging studies revealed endo- and exocytosis of F-UiO and
endosome acidification in real time. Fluorescently labeled NMOFs thus
represent a new class of nanosensors for intracellular pH sensing
and provide an excellent tool for studying NMOF–cell interactions
Nanoscale Metal–Organic Framework for Highly Effective Photodynamic Therapy of Resistant Head and Neck Cancer
Photodynamic
therapy (PDT) is an effective anticancer procedure
that relies on tumor localization of a photosensitizer followed by
light activation to generate cytotoxic reactive oxygen species (e.g., <sup>1</sup>O<sub>2</sub>). Here we report the rational design of a Hf–porphyrin
nanoscale metal–organic framework, DBP–UiO, as an exceptionally
effective photosensitizer for PDT of resistant head and neck cancer.
DBP–UiO efficiently generates <sup>1</sup>O<sub>2</sub> owing
to site isolation of porphyrin ligands, enhanced intersystem crossing
by heavy Hf centers, and facile <sup>1</sup>O<sub>2</sub> diffusion
through porous DBP–UiO nanoplates. Consequently, DBP–UiO
displayed greatly enhanced PDT efficacy both <i>in vitro</i> and <i>in vivo</i>, leading to complete tumor eradication
in half of the mice receiving a single DBP–UiO dose and a single
light exposure. NMOFs thus represent a new class of highly potent
PDT agents and hold great promise in treating resistant cancers in
the clinic
Nanoscale Metal–Organic Frameworks for the Co-Delivery of Cisplatin and Pooled siRNAs to Enhance Therapeutic Efficacy in Drug-Resistant Ovarian Cancer Cells
Ovarian cancer is the leading cause
of death among women with gynecological
malignancies. Acquired resistance to chemotherapy is a major limitation
for ovarian cancer treatment. We report here the first use of nanoscale
metal–organic frameworks (NMOFs) for the co-delivery of cisplatin
and pooled small interfering RNAs (siRNAs) to enhance therapeutic
efficacy by silencing multiple drug resistance (MDR) genes and resensitizing
resistant ovarian cancer cells to cisplatin treatment. UiO NMOFs with
hexagonal-plate morphologies were loaded with a cisplatin prodrug
and MDR gene-silencing siRNAs (Bcl-2, P-glycoprotein [P-gp], and survivin)
via encapsulation and surface coordination, respectively. NMOFs protect
siRNAs from nuclease degradation, enhance siRNA cellular uptake, and
promote siRNA escape from endosomes to silence MDR genes in cisplatin-resistant
ovarian cancer cells. Co-delivery of cisplatin and siRNAs with NMOFs
led to an order of magnitude enhancement in chemotherapeutic efficacy <i>in vitro</i>, as indicated by cell viability assay, DNA laddering,
and Annexin V staining. This work shows that NMOFs hold great promise
in the co-delivery of multiple therapeutics for effective treatment
of drug-resistant cancers
Mitochondria-Targeted Nanosystem Enhances Radio–Radiodynamic–Chemodynamic Therapy on Triple Negative Breast Cancer
Radiodynamic therapy (RDT), which produces 1O2 and other reactive oxygen species (ROS) in response
to X-rays, can
be used in conjunction with radiation therapy (RT) to drastically
lower X-ray dosage and reduce radio resistance associated with conventional
radiation treatment. However, radiation–radiodynamic therapy
(RT–RDT) is still impotent in a hypoxic environment in solid
tumors due to its oxygen-dependent nature. Chemodynamic therapy (CDT)
can generate reactive oxygen species and O2 by decomposing
H2O2 in hypoxic cells and thus potentiate RT–RDT
to achieve synergy. Herein, we developed a multifunctional nanosystem,
AuCu-Ce6-TPP (ACCT), for RT–RDT–CDT. Ce6 photosensitizers
were conjugated to AuCu nanoparticles via Au–S bonds to realize
radiodynamic sensitization. Cu can be oxidized by H2O2 and catalyze the degradation of H2O2 to generate •OH through the Fenton-like reaction
to realize CDT. Meanwhile, the degradation byproduct oxygen can alleviate
hypoxia while Au can consume glutathione to increase the oxidative
stress. We then attached mercaptoethyl-triphenylphosphonium (TPP-SH)
to the nanosystem, targeting ACCT to mitochondria (colocalization
Pearson coefficient 0.98) to directly disrupt mitochondrial membranes
and more efficiently induce apoptosis. We confirmed that ACCT efficiently
generates 1O2 and •OH upon
X-ray irradiation, resulting in strong anticancer efficacy in both
normoxic and hypoxic 4T1 cells. The down-regulation of hypoxia-inducible
factor 1α expression and reduction of intracellular H2O2 concentrations suggested that ACCT could significantly
alleviate hypoxia in 4T1 cells. ACCT-enhanced RT–RDT–CDT
can successfully shrink or remove tumors in radioresistant 4T1 tumor-bearing
mice upon 4 Gy of X-ray irradiation. Our work thus presents a new
strategy to treat radioresistant hypoxic tumors
Electron Crystallography Reveals Atomic Structures of Metal–Organic Nanoplates with M<sub>12</sub>(μ<sub>3</sub>‑O)<sub>8</sub>(μ<sub>3</sub>‑OH)<sub>8</sub>(μ<sub>2</sub>‑OH)<sub>6</sub> (M = Zr, Hf) Secondary Building Units
Nanoscale
metal–organic frameworks (nMOFs) have shown tremendous
potential in cancer therapy and biomedical imaging. However, their
small dimensions present a significant challenge in structure determination
by single-crystal X-ray crystallography. We report here the structural
determination of nMOFs by rotation electron diffraction (RED). Two
isostructural Zr- and Hf-based nMOFs with linear biphenyldicarboxylate
(BPDC) or bipyridinedicarboxylate (BPYDC) linkers are stable under
intense electron beams to allow the collection of high-quality RED
data, which reveal a MOF structure with M<sub>12</sub>(ÎĽ<sub>3</sub>-O)<sub>8</sub>(ÎĽ<sub>3</sub>-OH)<sub>8</sub>(ÎĽ<sub>2</sub>-OH)<sub>6</sub> (M = Zr, Hf) secondary building units (SBUs).
The nMOF structures differ significantly from their UiO bulk counterparts
with M<sub>6</sub>(ÎĽ<sub>3</sub>-O)<sub>4</sub>(ÎĽ<sub>3</sub>-OH)<sub>4</sub> SBUs and provide the foundation for clarifying
the structures of a series of previously reported nMOFs with significant
potential in cancer therapy and biological imaging. Our work clearly
demonstrates the power of RED in determining nMOF structures and elucidating
the formation mechanism of distinct nMOF morphologies
Synergistic Assembly of Heavy Metal Clusters and Luminescent Organic Bridging Ligands in Metal–Organic Frameworks for Highly Efficient X‑ray Scintillation
We have designed two metal–organic
frameworks (MOFs) to
efficiently convert X-ray to visible-light luminescence. The MOFs
are constructed from M<sub>6</sub>(ÎĽ<sub>3</sub>-O)<sub>4</sub>(ÎĽ<sub>3</sub>-OH)<sub>4</sub>(carboxylate)<sub>12</sub> (M
= Hf or Zr) secondary building units (SBUs) and anthracene-based dicarboxylate
bridging ligands. The high atomic number of Zr and Hf in the SBUs
serves as effective X-ray antenna by absorbing X-ray photons and converting
them to fast electrons through the photoelectric effect. The generated
electrons then excite multiple anthracene-based emitters in the MOF
through inelastic scattering, leading to efficient generation of detectable
photons in the visible spectrum. The MOF materials thus serve as efficient
X-ray scintillators via synergistic X-ray absorption by the metal-cluster
SBUs and optical emission by the bridging ligands
Nanoscale Metal–Organic Frameworks for Ratiometric Oxygen Sensing in Live Cells
We
report the design of a phosphorescence/fluorescence dual-emissive
nanoscale metal–organic framework (NMOF), R-UiO, as an intracellular
oxygen (O<sub>2</sub>) sensor. R-UiO contains a PtÂ(II)-porphyrin ligand
as an O<sub>2</sub>-sensitive probe and a Rhodamine-B isothiocyanate
ligand as an O<sub>2</sub>-insensitive reference probe. It exhibits
good crystallinity, high stability, and excellent ratiometric luminescence
response to O<sub>2</sub> partial pressure. <i>In vitro</i> experiments confirmed the applicability of R-UiO as an intracellular
O<sub>2</sub> biosensor. This work is the first report of a NMOF-based
intracellular oxygen sensor and should inspire the design of ratiometric
NMOF sensors for other important analytes in biological systems
Chlorin-Based Nanoscale Metal–Organic Framework Systemically Rejects Colorectal Cancers via Synergistic Photodynamic Therapy and Checkpoint Blockade Immunotherapy
Photodynamic
therapy (PDT) can destroy local tumors and minimize
normal tissue damage, but is ineffective at eliminating metastases.
Checkpoint blockade immunotherapy has enjoyed recent success in the
clinic, but only elicits limited rates of systemic antitumor response
for most cancers due to insufficient activation of the host immune
system. Here we describe a treatment strategy that combines PDT by
a new chlorin-based nanoscale metal–organic framework (nMOF),
TBC-Hf, and a small-molecule immunotherapy agent that inhibits indoleamine
2,3-dioxygenase (IDO), encapsulated in the nMOF channels to induce
systemic antitumor immunity. The synergistic combination therapy achieved
effective local and distant tumor rejection in colorectal cancer models.
We detected increased T cell infiltration in the tumor microenvironment
after activation of the immune system with the combination of IDO
inhibition by the small-molecule immunotherapy agent and immunogenic
cell death induced by PDT. We also elucidated the underlying immunological
mechanisms and revealed compensatory roles of neutrophils and B cells
in presenting tumor-associated antigens to T cells in this combination
therapy. We believe that nMOF-enabled PDT has the potential to significantly
enhance checkpoint blockade cancer immunotherapy, affording clinical
benefits for the treatment of many difficult-to-treat cancers