Transcriptome sequencing of olfactory-related genes in olfactory transduction of large yellow croaker (Larimichthy crocea) in response to bile salts

Fish produce and release bile salts as chemical signalling substances that act as sensitive olfactory stimuli. To investigate how bile salts affect olfactory signal transduction in large yellow croaker (Larimichthy crocea), deep sequencing of olfactory epithelium was conducted to analyse olfactory-related genes in olfactory transduction. Sodium cholates (SAS) have typical bile salt chemical structures, hence we used four different concentrations of SAS to stimulate L. crocea, and the fish displayed a significant behavioural preference for 0.30% SAS. We then sequenced olfactory epithelium tissues, and identified 9938 unigenes that were significantly differentially expressed between SAS-stimulated and control groups, including 9055 up-regulated and 883 down-regulated unigenes. Subsequent Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses found eight categories linked to the olfactory transduction pathway that was highly enriched with some differentially expressed genes (DEGs), including the olfactory receptor (OR), Adenylate cyclase type 3 (ADCY3) and Calmodulin (CALM). Genes in these categories were analysed by RT-qPCR, which revealed aspects of the pathway transformation between odor detection, and recovery and adaptation. The results provide new insight into the effects of bile salt stimulation in olfactory molecular mechanisms in fishes, and expands our knowledge of olfactory transduction, and signal generation and decline

Study on the phytoplankton in a large reservoir

A comprehensive study on the community structure and function of the phytoplankton in Taipinghu Reservoir, the largest reservoir (9400 ha) in Anhui Province, China, was carried out during 1985-1986. A total of 175 species of algae belonging to 8 phyla and 87 genera was noted. The composition of phytoplankton was dominated by species of Chlorophyta, Cyanophyta and Bacillariophyta. The species number, cell density and biomass of the three groups were respectively 88%, 86.5% and 78.9% of the total phytoplankton. The weighted annual average biomass was 1.52 mg/L and cell density was 1.43 times 10-6 ind/L. The growth maximum was observed in summer. Diatoms were abundant in the region adjoining a river. A large number of flagellated algae such as Euglena and Chromulina occurred in the artificial fish culture bay. It can be inferred from the algal composition and total nitrogen concentration (1.48 mg/L) that this reservoir is a mesotrophic water body that had undergone slight natural eutrophication. Presented are an equation for the relation between cell density and biomass of algae and a newly developed method based on the energy flow principle in ecology for theoretically estimating algal fishery potential. The algae volumes of 128 species were measured for calculating the biomass

Phytoplankton community and algal growth potential in Taipinghu Reservoir, Anhui Province, China

An integrated investigation of the community structure and growth potential of algae in Taipinghu Reservoir, Anhui Province, China, was conducted from July 1993 to October 1994. A total of 112 algal species belonging to eight phyla were observed. Green algae were the dominant population, representing 55% of the total recorded taxa. Diatoms and blue-green algae formed the second and the third largest algal groups, respectively. The total species and population of algae were relatively steady in spring, summer and autumn, but exhibited some variety in winter. Compared to earlier data, the species richness decreased by 36% and the number of genera decreased by 23% over an eight-year period. The maximum values of the annual mean cell count, biomass and chlorophyll a concentration appeared in summer and the minimum in winter. The algal growth potential of the original water without supplemental nutrients was much lower than that of the control culture, but equal to or a little higher than the control culture which was supplemented with complete nutrients. The study results imply that phosphorus is the limiting nutrient for algal growth and that the trophic status of the reservoir is changing from mesotrophic to eutrophic. Based on these data, some advice on how to manage the reservoir is offered

Institutional Adoption and Implementation of Blended Learning in the Era of Intelligent Education

Blended learning (BL) reform is one of the vital methods to improve the teaching quality of institutions in the intelligent education era. However, institutions are always faced with many obstacles (instructors’ reluctance/inability to change, etc.) in conducting the reform. What is worse, very few studies have reported the design and effect of such a transformation. This study designed an intervention of institutional BL reform by making a unified deployment based on Graham et al.’s BL adoption framework: identifying strategy, structure, and support issues at three developmental stages. More than 900 courses (involving more than 14,000 students and more than 2000 instructors) within S university were taken as a sample. A quasi-experiment was designed to investigate the effect of the intervention on S university’s BL course transformation, students’ learning, instructors’ professional development, etc. Course logs, responses to students’ course evaluation forms, and instructors’ questionnaires were collected and analyzed. Results indicated that S university systematically conducted the BL transformation and gradually reached the mature implementation stage within 7 years. This study contributes to the literature by reporting a best practice of BL institutional adoption. Three implications, relating to strategy, structure, and support, were drawn to shed light for other institutions in moving forward on BL adoption

Institutional Adoption and Implementation of Blended Learning in the Era of Intelligent Education

Blended learning (BL) reform is one of the vital methods to improve the teaching quality of institutions in the intelligent education era. However, institutions are always faced with many obstacles (instructors’ reluctance/inability to change, etc.) in conducting the reform. What is worse, very few studies have reported the design and effect of such a transformation. This study designed an intervention of institutional BL reform by making a unified deployment based on Graham et al.’s BL adoption framework: identifying strategy, structure, and support issues at three developmental stages. More than 900 courses (involving more than 14,000 students and more than 2000 instructors) within S university were taken as a sample. A quasi-experiment was designed to investigate the effect of the intervention on S university’s BL course transformation, students’ learning, instructors’ professional development, etc. Course logs, responses to students’ course evaluation forms, and instructors’ questionnaires were collected and analyzed. Results indicated that S university systematically conducted the BL transformation and gradually reached the mature implementation stage within 7 years. This study contributes to the literature by reporting a best practice of BL institutional adoption. Three implications, relating to strategy, structure, and support, were drawn to shed light for other institutions in moving forward on BL adoption