822 research outputs found
Constitutive cytoplasmic localization of p21Waf1/Cip1 affects the apoptotic process in monocytic leukaemia
In the present study, we analysed the expression and localization of p21Waf1/Cip1 in normal and malignant haematopoietic cells. We demonstrate that in normal monocytic cells, protein kinase C (PKC)-induced p21 gene activation, which is nuclear factor-κB (NF-κB) independent, results in predominantly cytoplasmic localized p21 protein. In acute monocytic leukaemia (M4, M5), monocytic blasts (N=12) show constitutive cytoplasmic p21 expression in 75% of the cases, while in myeloid leukaemic blasts (N=10), low nuclear and cytoplasmic localization of p21 could be detected, which is also PKC dependent. Constitutive p21 expression in monocytic leukaemia might have important antiapoptotic functions. This is supported by the finding that in U937 cells overexpressing p21, VP16-induced apoptosis is significantly reduced (20.0±0.9 vs 55.8±3.8%, P<0.01, N=5), reflected by a reduced phosphorylation of p38 and JNK. Similarly, AML blasts with high cytoplasmic p21 were less sensitive to VP16-induced apoptosis as compared to AML cases with low or undetectable p21 expression (42.25 vs 12.3%, P<0.01). Moreover, complex formation between p21 and ASK1 could be demonstrated in AML cells, by means of coimmunoprecipitation. In summary, these results indicate that p21 has an antiapoptotic role in monocytic leukaemia, and that p21 expression is regulated in a PKC-dependent and NF-κB independent manner.
Bandstructure and Fermi Surfaces of CeRh3B2
The electronic bandstructure and the Fermi surfaces of ferromagnetic CeRh3B2
are calculated by using FLAPW and LSDA+U method. As assuming several kinds of
the ground state to describe the 4f electronic state, we propose a fully
orbital- and spin-polarized state |lz=0, sx=1/2> as the ground state, instead
of the conventional LS-coupled CEF ground state, generally expected in typical
4f compounds. This is supported by the fact that both the observed magnetic
moment and the observed dHvA frequencies are well explained by the calculated
electronic structure and the Fermi surfaces. The unconventional ground state is
stabilized by the strong 4f-4f direct mixing between the neighbored Ce atoms
along the extremely small distance along the c-axis in the hexagonal crystal
cell.Comment: 8 pages, 9 figures, submitted to JPSJ (accepted and will appear in
the issue of Vol.79, No.4
Pressure-induced anomalous valence crossover in cubic YbCu5-based compounds
A pressure-induced anomalous valence crossover without structural phase transition is observed in archetypal cubic YbCu5 based heavy Fermion systems. The Yb valence is found to decrease with increasing pressure, indicating a pressure-induced crossover from a localized 4f (13) state to the valence fluctuation regime, which is not expected for Yb systems with conventional c-f hybridization. This result further highlights the remarkable singularity of the valence behavior in compressed YbCu5-based compounds. The intermetallics Yb2Pd2Sn, which shows two quantum critical points (QCP) under pressure and has been proposed as a potential candidate for a reentrant Yb(2+) state at high pressure, was also studied for comparison. In this compound, the Yb valence monotonically increases with pressure, disproving a scenario of a reentrant non-magnetic Yb(2+) state at the second QCP
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