Do Generalist Tiger Swallowtail Butterfly Females Select Dark Green Leaves Over Yellowish – Or Reddish-Green Leaves for Oviposition?

In late August and September, using leaves from the same branches, the polyphagous North American swallowtail butterfly species Papilio glaucus L. (Lepidoptera: Papilionidae) is shown to select mature dark green leaves of their host plants white ash, Fraxinus americana L. (Oleaceae) and tulip tree, Liriodendron tulipifera L. (Magnoliaceae) rather than the pale green or yellowish- green mature leaves in laboratory oviposition arenas. In early August, similar results were observed for black cherry, Prunus serotina Ehrh. (Rosaceae). Dark green leaves were preferred over light green and yellowish green leaves. These green leaves of black cherry were the most nutritious leaves for larval growth indicating a clear correlation between adult preference and larval performance on this plant. However, tulip tree leaves in the summer did not elicit different oviposition responses between green and light green leaves. A field evaluation of oviposition preferences for young expanding reddish leaves of red bay, Persea borbonia (L.) Spreng (Lauraceae) versus slightly older expanded green leaves of the same branch also suggested avoidance of “young” red leaves in Florida by Papilio troilus L. and Papilio palamedes Drury during the spring season (March-April)

Do Generalist Tiger Swallowtail Butterfly Females Select Dark Green Leaves Over Yellowish – Or Reddish-Green Leaves for Oviposition?

In late August and September, using leaves from the same branches, the polyphagous North American swallowtail butterfly species Papilio glaucus L. (Lepidoptera: Papilionidae) is shown to select mature dark green leaves of their host plants white ash, Fraxinus americana L. (Oleaceae) and tulip tree, Liriodendron tulipifera L. (Magnoliaceae) rather than the pale green or yellowish- green mature leaves in laboratory oviposition arenas. In early August, similar results were observed for black cherry, Prunus serotina Ehrh. (Rosaceae). Dark green leaves were preferred over light green and yellowish green leaves. These green leaves of black cherry were the most nutritious leaves for larval growth indicating a clear correlation between adult preference and larval performance on this plant. However, tulip tree leaves in the summer did not elicit different oviposition responses between green and light green leaves. A field evaluation of oviposition preferences for young expanding reddish leaves of red bay, Persea borbonia (L.) Spreng (Lauraceae) versus slightly older expanded green leaves of the same branch also suggested avoidance of “young” red leaves in Florida by Papilio troilus L. and Papilio palamedes Drury during the spring season (March-April)

Data associated with "Robust Structured Illumination Microscopy with Bayesian Noise Control"

<p>Experimental and synthetic data saved in tiff and/or zarr formats and SIM reconstruction scripts written in Python (Wiener and FISTA-SIM0. These also include estimated SIM patterns which are needed for B-SIM</p><ul><li>Synthetic data consisting of variably spaced line pairs. Found in <a href="https://zenodo.org/uploads/10037823">2023_10_02_synthetic_line_pairs.zip</a></li><li>Experimental data. Fluorescence images of one of the variably spaced line pair patterns on an ArgoSIM calibration slide. Found in <a href="https://zenodo.org/uploads/10037823">2023_08_02_folder=002_argosim_slide.zip</a></li><li>Experimental data. MitoTracker Red labelled mitochondria in live HeLa cells. Found in <a href="2023_08_07_folder=011_mitos_live_hela.zip">2023_08_07_folder=011_mitos_live_hela.zip</a></li><li>Camera calibration maps, including gain, variance, and offset. Found in <a href="camera_calibration.zip">camera_calibration.zip</a></li></ul&gt

Fourier synthesis optical diffraction tomography for kilohertz rate volumetric imaging

Many biological processes occur at high speeds in complex 3D environments, and developing imaging techniques capable of elucidating their dynamics is an outstanding challenge. One outstanding question is understanding the interactions of active swimmers with the local physics of their viscous 3D environment. Here, we address this challenge by developing a multiplexed quantitative phase imaging approach capable of recording the 3D refractive index at kilohertz rates. Specifically, we develop Fourier synthesis optical diffraction tomography (FS-ODT), a new pattern generation and inverse computational strategy for ODT. In FS-ODT, we multiplex tens of beam angles to expand the system field of view and increase the information in single images, thereby increasing the tomogram acquisition rate. We further show that FS-ODT can simultaneously multiplex beam angles and positions to synthesize a large field of view at kilohertz framerates. We verify FS-ODT performance by imaging samples of known composition and accurately recovering the refractive index for multiplexing conditions. We then demonstrate the capabilities of FS-ODT for imaging fast diffusing microspheres in solution and single-cellular bacterial swimmers. FS-ODT is a promising approach for unlocking challenging imaging regimes that have been little explored, including measuring 3D flow fields generated by microswimmers