Draft genome sequence of root-associated sugarcane growth-promoting microbispora sp. strain GKU 823

The endophytic plant growth-promoting Microbispora sp. strain GKU 823 was isolated from the roots of sugarcane cultivated in Thailand. It has an estimated 9.4-Mbp genome and a G+C content of 71.3%. The genome sequence reveals several genes associated with plant growth-promoting traits and extensive specialized metabolite biosynthesis

Draft genome sequence of root-associated sugarcane growth promoting Microbispora sp. GKU 823

The endophytic plant growth promoting Microbispora sp. GKU 823 was isolated from the roots of sugarcane cultivated in Thailand. It has an estimated 9.4 Mbp genome and a G+C content of 71.3%. The genome sequence reveals several genes associated with plant growth-promoting traits and extensive secondary metabolite biosyntheses

Draft genome sequence of plant growth-promoting endophytic Streptomyces sp. GKU 895 isolated from the roots of sugarcane

Streptomyces sp. GKU 895 is an endophytic actinomycete isolated from the roots of sugarcane. GKU 895 has a genome of 8.3 Mbp and the genome exhibits adaptations related to plant growth-promoting activity. It also has extensive specialized metabolite biosynthetic gene clusters apparent in its genome

Integrating perspectives in actinomycete research: an ActinoBase review of 2020-21

Last year ActinoBase, a Wiki-style initiative supported by the UK Microbiology Society, published a review highlighting the research of particular interest to the actinomycete community. Here, we present the second ActinoBase review showcasing selected reports published in 2020 and early 2021, integrating perspectives in the actinomycete field. Actinomycetes are well-known for their unsurpassed ability to produce specialised metabolites, of which many are used as therapeutic agents with antibacterial, antifungal, or immunosuppressive activities. Much research is carried out to understand the purpose of these metabolites in the environment, either within communities or in host interactions. Moreover, many efforts have been placed in developing computational tools to handle big data, simplify experimental design, and find new biosynthetic gene cluster prioritisation strategies. Alongside, synthetic biology has provided advances in tools to elucidate the biosynthesis of these metabolites. Additionally, there are still mysteries to be uncovered in understanding the fundamentals of filamentous actinomycetes' developmental cycle and regulation of their metabolism. This review focuses on research using integrative methodologies and approaches to understand the bigger picture of actinomycete biology, covering four research areas: i) technology and methodology; ii) specialised metabolites; iii) development and regulation; and iv) ecology and host interactions

Transcriptome landscapes of salt-susceptible rice cultivar IR29 associated with a plant growth promoting endophytic streptomyces

Plant growth-promoting endophytic (PGPE) actinomycetes have been known to enhance plant growth and mitigate plant from abiotic stresses via their PGP-traits. In this study, PGPE Streptomyces sp. GKU 895 promoted growth and alleviated salt tolerance of salt-susceptible rice cultivar IR29 by augmentation of plant weight and declined ROS after irrigation with 150 mM NaCl in a pot experiment. Transcriptome analysis of IR29 exposed to the combination of strain GKU 895 and salinity demonstrated up and downregulated differentially expressed genes (DEGs) classified by gene ontology and plant reactome. Streptomyces sp. GKU 895 induced changes in expression of rice genes including transcription factors under salt treatment which involved in growth and development, photosynthesis, plant hormones, ROS scavenging, ion transport and homeostasis, and plant–microbe interactions regarding pathogenesis- and symbiosis-related proteins. Taken together, these data demonstrate that PGPE Streptomyces sp. GKU 895 colonized and enhanced growth of rice IR29 and triggered salt tolerance phenotype. Our findings suggest that utilisation of beneficial endophytes in the saline fields could allow for the use of such marginal soils for growing rice and possibly other crops

Meteorological data and sago palm-growing soil properties collected in the summer and rainy seasons.

Data are mean ± S.D. of five independent replicates (n = 5). Asterisk symbol represents the significant P-value (P<0.05).</p

Taxonomic distribution and average relative abundance of the uncultured bacterial communities.

(A) Distribution of bacterial taxonomic community and (B) and average relative abundance of uncultured bacteria (relative abundance ≥1%, n = 5) found in Phatthalung sago palm-growing soils collected during the summer (S1-S5) and rainy (R1-R5) seasons. Colour families display unique microbial phyla and distinct shades reflect microbial family. Low relative abundance refers to the summation of all taxa that failed to reach the 1% cut-off.</p

Microbial alpha and beta diversity in the soil samples collected in the sago palm forest.

Boxplot displays (A) the Shannon index, (B) Faith’s phylogenetic diversity, and (C) principal coordinates analysis (PCA) of a Bray-Curtis dissimilarity matrix.</p

Supporting Fig.

Environmental variations have been observed to influence bacterial community composition, thereby impacting biological activities in the soil. Together, the information on bacterial functional groups in Phatthalung sago palm-growing soils remains limited. In this work, the core soil bacterial community in the Phatthalung sago palm-growing areas during both the summer and rainy seasons was examined using V3-V4 amplicon sequencing. Our findings demonstrated that the seasons had no significant effects on the alpha diversity, but the beta diversity of the community was influenced by seasonal variations. The bacteria in the phyla Acidobacteriota, Actinobacteriota, Chloroflexi, Methylomirabilota, Planctomycetota, and Proteobacteria were predominantly identified across the soil samples. Among these, 26 genera were classified as a core microbiome, mostly belonging to uncultured bacteria. Gene functions related to photorespiration and methanogenesis were enriched in both seasons. Genes related to aerobic chemoheterotrophy metabolisms and nitrogen fixation were more abundant in the rainy season soils, while, human pathogen pneumonia-related genes were overrepresented in the summer season. The investigation not only provides into the bacterial composition inherent to the sago palm-cultivated soil but also the gene functions during the shift in seasons.</div

Five different sites of soil samples collected in the sago palm forest.

Soil samples were collected at Ban Hua Phru, Khuan Khanun, Phatthalung, Thailand during the summer (orange triangle, S1 to S5) and the rainy seasons (green circle, R1 to R5).</p