Phenolic and multi-elemental profiles as a tool for quality assessment of Serbian blackberry wines

Blackberry wine is produced by a yeast fermentation of natural saccharides present in blackberry juice. This wine is traditionally consumed in Balkans, mostly as a dessert wine, but in recent years it is gaining more attention due to unique flavour and potential health benefits. Blackberries are a natural source of many minerals and bioactive phytochemicals such as vitamins, folic acid, anthocyanins, and phenolics1,2. Considering biological potential of blackberries and their products aim of this study was to evaluate quality of Serbian blackberry wines. This quality assessment was achieved through determination of: (a) the phenolic profile by high-performance thin layer chromatography (HPTLC); (b) total phenolic (TPC) and anthocyanin content (TAC); (c) in vitro antioxidant activity by DPPH and ABTS radical scavenging assays and total antioxidant capacity (d) in vitro antimicrobial activity by the agar diffusion test; and (e) the content of macro, micro and toxic elements by inductively coupled plasma mass spectrometry (ICP-MS). Twelve blackberry wines from different geographical regions were tested indicating various phenolic profiles and great antioxidant and antimicrobial activity. Strong positive correlation was confirmed between TPC and antioxidant activity determined by DPPH and ABTS assays. Additionally, high levels of antibacterial activity were shown against all six tested strains, but primarily against Staphylococcus aureus and Micrococcus lysodeikticus. Samples were rich in macroelements, such as Ca, K, Mg, Na, P and Zn, and microelements Cu and Fe, while Mn was present in higher concentration only in one sample. Toxic metals Cd and Pb were present in low concertation in all samples. These results indicate great quality of Serbian blackberry wines concerning minerals, phenolics, antioxidant and antimicrobial activity, which support its health potential

Supplementary material for the article: Krstić, Đ.; Vukojević, V.; Mutić, J.; Fotirić Akšić, M.; Ličina, V.; Milojković-Opsenica, D.; Trifković, J. Distribution of Elements in Seeds of Some Wild and Cultivated Fruits. Nutrition and Authenticity Aspects. Journal of the Science of Food and Agriculture 2019, 99 (2), 546–554. https://doi.org/10.1002/jsfa.9213

Supplementary material for: [https://doi.org/10.1002/jsfa.9213]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/343]Related to accepted version: [http://cherry.chem.bg.ac.rs/handle/123456789/3200

Supplementary material for the article: Krstić, Đ.; Vukojević, V.; Mutić, J.; Fotirić Akšić, M.; Ličina, V.; Milojković-Opsenica, D.; Trifković, J. Distribution of Elements in Seeds of Some Wild and Cultivated Fruits. Nutrition and Authenticity Aspects. Journal of the Science of Food and Agriculture 2019, 99 (2), 546–554. https://doi.org/10.1002/jsfa.9213

Supplementary material for: [https://doi.org/10.1002/jsfa.9213]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/343]Related to accepted version: [http://cherry.chem.bg.ac.rs/handle/123456789/3200

Supplementary data for the article: Stojsavljević, A.; Rovčanin, B.; Krstić, Đ.; Borković-Mitić, S.; Paunović, I.; Kodranov, I.; Gavrović-Jankulović, M.; Manojlović, D. Evaluation of Trace Metals in Thyroid Tissues: Comparative Analysis with Benign and Malignant Thyroid Diseases. Ecotoxicology and Environmental Safety 2019, 183. https://doi.org/10.1016/j.ecoenv.2019.109479

Supplementary material for: [10.1016/j.ecoenv.2019.109479]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/3285

Supplementary data for the article: Jagodić, J.; Rovčanin, B.; Krstić, Đ.; Paunović, I.; Živaljević, V.; Manojlović, D.; Stojsavljević, A. Elemental Profiling of Adrenal Adenomas in Solid Tissue and Blood Samples by ICP-MS and ICP-OES. Microchemical Journal 2021, 165, 106194. https://doi.org/10.1016/j.microc.2021.106194.

Supplementary material for: [https://doi.org/10.1016/j.microc.2021.106194]Related to published version: [https://cherry.chem.bg.ac.rs/handle/123456789/4530

Supplementary material for the article: Stojsavljević, A.; Rovčanin, B.; Krstić; Jagodić, J.; Borković-Mitić, S.; Paunović, I.; Živaljević, V.; Mitić, B.; Gavrović-Jankulović, M.; Manojlović, D. Cadmium as Main Endocrine Disruptor in Papillary Thyroid Carcinoma and the Significance of Cd/Se Ratio for Thyroid Tissue Pathophysiology. Journal of Trace Elements in Medicine and Biology 2019, 55, 190–195. https://doi.org/10.1016/j.jtemb.2019.06.009

Supplementary material for: [https://doi.org/10.1016/j.jtemb.2019.06.009]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/3293

Polyphenolic Profile of Maize Seedlings Treated with 24-Epibrassinolide

High-performance thin-layer chromatography (HPTLC) combined with image analysis and pattern recognition methods were used for fingerprinting of phenolic compounds present in seedlings of two maize genotypes ZP 434 (new generation hybrid, drought tolerant) and ZP 704 (older generation hybrid, drought sensitive) treated with different concentrations of 24-epibrassinolide. This is the first report of TLC chromatographic profile of phenolics' mixtures in maize seed extracts influenced by brassinosteroid phytohormones. Nine samples of shoot of seedlings for the whole concentration range of phytohormones (5.2 x 10(-7) -5.2 x 10(-15) M), one sample of root of seedlings treated with 5.2 x 10(-15) M 24-epibrassinolide, and the control samples of nontreated seedlings, for both genotypes, were analyzed. Phenolic profiles of root extracts indicate the absence of more polar compounds such as phenolic acids and glycosides present in shoot of seedlings. Also, hormones applied in higher concentrations have an inhibiting effect on the content of phenolics in ZP 434. Application of chemometric methods enables characterization of particular genotype of maize according to its phenolic profile

Supplementary data for the article: Stojsavljević, A.; Rovčanin, B.; Krstić, Đ.; Borković-Mitić, S.; Paunović, I.; Kodranov, I.; Gavrović-Jankulović, M.; Manojlović, D. Evaluation of Trace Metals in Thyroid Tissues: Comparative Analysis with Benign and Malignant Thyroid Diseases. Ecotoxicology and Environmental Safety 2019, 183. https://doi.org/10.1016/j.ecoenv.2019.109479

Supplementary material for: [10.1016/j.ecoenv.2019.109479]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/3285

Supplementary material for the article: Stojsavljević, A.; Rovčanin, B.; Krstić; Jagodić, J.; Borković-Mitić, S.; Paunović, I.; Živaljević, V.; Mitić, B.; Gavrović-Jankulović, M.; Manojlović, D. Cadmium as Main Endocrine Disruptor in Papillary Thyroid Carcinoma and the Significance of Cd/Se Ratio for Thyroid Tissue Pathophysiology. Journal of Trace Elements in Medicine and Biology 2019, 55, 190–195. https://doi.org/10.1016/j.jtemb.2019.06.009

Supplementary material for: [https://doi.org/10.1016/j.jtemb.2019.06.009]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/3293

Multi-elemental Analysis, Pattern Recognition Techniques of Wild and Cultivated Rosehips from Serbia, and Nutritional Aspect

Twenty-six major and trace elements, in the seed and the mesocarp of wild and cultivated rosehips from different locations in Serbia, were quantified by means of inductively coupled plasma–atomic emission spectroscopy (ICP–AES). The cultivated rosehip was investigated for the first time. In both wild and cultivated rosehips, the most abundant elements were K and Ca. Among trace elements, Mn (in both seed and mesocarp); Fe (in seeds); and B, Ba, and Sr (in mesocarp) were quantified in the highest concentrations. The higher content of Cu, K, Mn, P, and S in the seed of cultivated rosehip, as well as Ca, Mg, and Sr in the mesocarp of wild rosehip, was observed, both significant at p < 0.05. Additionally, differences between the seed and the mesocarp of studied rosehip were noticed in the content of B, Cu, Fe, K, Ni, P, S, Sr, and Ti (p < 0.05). Nutritional assessment revealed that both wild and cultivated rosehips are a valuable source of essential elements (Ca, Cu, K, Mg, Mn, and P). The absence of toxic and potentially toxic elements additionally contributes to the quality of studied Serbian rosehip. A high impact of factors such as variety, location, as well as their interaction on the content of elements in cultivated rosehip mesocarp and seed was observed. Pattern recognition techniques, principal component analysis (PCA), and hierarchical cluster analysis (HCA) were applied in order to provide insight into similarities among the analyzed samples