487 research outputs found
Difference in membrane repair capacity between cancer cell lines and a normal cell line
Electroporation-based treatments and other therapies that permeabilize the plasma membrane have been shown to be more devastating to malignant cells than to normal cells. In this study, we asked if a difference in repair capacity could explain this observed difference in sensitivity. Membrane repair was investigated by disrupting the plasma membrane using laser followed by monitoring fluorescent dye entry over time in seven cancer cell lines, an immortalized cell line, and a normal primary cell line. The kinetics of repair in living cells can be directly recorded using this technique, providing a sensitive index of repair capacity. The normal primary cell line of all tested cell lines exhibited the slowest rate of dye entry after laser disruption and lowest level of dye uptake. Significantly, more rapid dye uptake and a higher total level of dye uptake occurred in six of the seven tested cancer cell lines (p < 0.05) as well as the immortalized cell line (p < 0.001). This difference in sensitivity was also observed when a viability assay was performed one day after plasma membrane permeabilization by electroporation. Viability in the primary normal cell line (98 % viable cells) was higher than in the three tested cancer cell lines (81â88 % viable cells). These data suggest more effective membrane repair in normal, primary cells and supplement previous explanations why electroporation-based therapies and other therapies permeabilizing the plasma membrane are more effective on malignant cells compared to normal cells in cancer treatment
A new methodology to simulate subglacial deformation of water-saturated granular material
The dynamics of glaciers are to a large degree governed by processes
operating at the iceâbed interface, and one of the primary
mechanisms of glacier flow over soft unconsolidated sediments is
subglacial deformation. However, it has proven difficult to
constrain the mechanical response of subglacial sediment to the
shear stress of an overriding glacier. In this study, we present
a new methodology designed to simulate subglacial deformation using
a coupled numerical model for computational experiments on
grain-fluid mixtures. The granular phase is simulated on a per-grain
basis by the discrete element method. The pore water is modeled as
a compressible Newtonian fluid without inertia. The numerical
approach allows close monitoring of the internal behavior under
a range of conditions.
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Our computational experiments support the findings of previous studies
where the rheology of a slowly deforming water-saturated granular bed in the
steady state generally conforms to the rate-independent plastic rheology.
Before this so-called critical state, deformation is in many cases accompanied
by volumetric changes as grain rearrangement in active shear zones changes the
local porosity. For previously consolidated beds porosity
increases can cause local pore-pressure decline, dependent on till
permeability and shear rate. We observe that the pore-water pressure reduction
strengthens inter-granular contacts, which results in increased shear strength
of the granular material. In contrast, weakening takes place when shear
deformation causes consolidation of dilated sediments or during rapid fabric
development. Both processes of strengthening and weakening depend inversely on
the sediment permeability and are transient phenomena tied to the porosity
changes during the early stages of shear.
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We find that the transient strengthening and weakening in turn influences the
distribution of shear strain in the granular bed. Dilatant strengthening has
the ability to distribute strain during early deformation to large depths, if
sediment dilatancy causes the water pressure at the iceâbed interface to
decline. Oppositely, if the iceâbed interface is hydrologically stable the
strengthening process is minimal and instead causes shallow deformation. The
depth of deformation in subglacial beds thus seems to be governed by not only
local grain and pore-water feedbacks but also larger-scale hydrological
properties at the ice base
Dealing with a traumatic past: the victim hearings of the South African truth and reconciliation commission and their reconciliation discourse
In the final years of the twentieth and the beginning of the twenty-first century, there has been a worldwide tendency to approach conflict resolution from a restorative rather than from a retributive perspective. The South African Truth and Reconciliation Commission (TRC), with its principle of 'amnesty for truth' was a turning point. Based on my discursive research of the TRC victim hearings, I would argue that it was on a discursive level in particular that the Truth Commission has exerted/is still exerting a long-lasting impact on South African society. In this article, three of these features will be highlighted and illustrated: firstly, the TRC provided a discursive forum for thousands of ordinary citizens. Secondly, by means of testimonies from apartheid victims and perpetrators, the TRC composed an officially recognised archive of the apartheid past. Thirdly, the reconciliation discourse created at the TRC victim hearings formed a template for talking about a traumatic past, and it opened up the debate on reconciliation. By discussing these three features and their social impact, it will become clear that the way in which the apartheid past was remembered at the victim hearings seemed to have been determined, not so much by political concerns, but mainly by social needs
Water Solubilization Using Nonionic Surfactants from Renewable Sources in Microemulsion Systems
In this study the effect of temperature, NaCl and oils (hydrocarbons: C8âC16) on the formation and solubilization capacity of the systems of oil/monoacylglycerols (MAG):ethoxylated fatty alcohols (CEO20)/propylene glycol (PG)/water was investigated. The effects of the surfactant mixture on the phase behavior and the concentration of water or oil in the systems were studied at three temperatures (50, 55, 60 °C) and with varied NaCl solutions (0.5; 2; 11%). Electrical conductivity measurement, FTIR spectroscopy and the DSC method were applied to determine the structure and type of the microemulsions formed. The dimension of the microemulsion droplets was characterized by dynamic light scattering. It has been stated that the concentration of CEO20 has a strong influence on the shape and extent of the microemulsion areas. Addition of a nonionic surfactant to the mixture with MAG promotes an increase in the area of microemulsion formation in the phase diagrams, and these areas of isotropic region did not change considerably depending on the temperature, NaCl solution and oil type. It was found that, depending on the concentration of the surfactant mixture, it was possible to obtain U-type microemulsions with dispersed particles size distribution ranging from 25 to 50 nm and consisting of about 30â32% of the water phase in the systems. The conditions under which the microemulsion region was found (electrolyte and temperatureâinsensitive, comparatively low oil and surfactant concentration) could be highly useful in detergency
The quiet editor: Ivan VladislaviÄ and South African cultural production
This article examines the literary and sociological significance of Ivan VladislaviÄâs "double lifeâ (Lahire, 2010: n.p.) as both editor and writer. With reference to a number of his editorial roles as well as the joint projects he has worked on with writers and visual artists, the article considers how VladislaviÄâs work with others spreads symbolic value. Described by one of his clients as the âquiet editorâ, VladislaviÄ can be read as a new kind of author; what he terms âcreative editingâ (Steyn, 2012: n.p.) as a new kind of writing, through which more traditional models of authorship and literary production are thrown into question â less Bourdieuâs (1984) âfield of literary productionâ or Casanovaâs (2004: 82) âworld literary spaceâ, red in tooth and claw, amd more Howard Beckerâs âart worldâ: a convivial ânetwork of cooperating people, all of whose work is essential to the final outcomeâ (1982: 25)
First report of the presence of hepatitis E virus in Scottish harvested shellfish purchased at retail level
The racist bodily imaginary: the image of the body-in-pieces in (post)apartheid culture
This paper outlines a reoccurring motif within the racist imaginary of (post)apartheid culture: the black body-in-pieces. This disturbing visual idiom is approached from three conceptual perspectives. By linking ideas prevalent in Frantz Fanonâs description of colonial racism with psychoanalytic concepts such as Lacanâs notion of the corps morcelĂŠ, the paper offers, firstly, an account of the black body-in-pieces as fantasmatic preoccupation of the (post)apartheid imaginary. The role of such images is approached, secondly, through the lens of affect theory which eschews a representational âreadingâ of such images in favour of attention to their asignifying intensities and the role they play in effectively constituting such bodies. Lastly, Judith Butlerâs discussion of war photography and the conditions of grievability introduces an ethical dimension to the discussion and helps draw attention to the unsavory relations of enjoyment occasioned by such images
Extrasystoles for fluid responsiveness prediction in critically ill patients
Background: Fluid responsiveness prediction with continuously available monitoring is an unsettled matter for the vast majority of critically ill patients, and development of new and reliable methods is desired. We hypothesized that the post-ectopic beat, which is associated with increased preload, could be analyzed in relation to preceding sinus beats and that the change in cardiac performance (e.g., systolic blood pressure) at the post-ectopic beat could predict fluid responsiveness. Methods: Critically ill patients were observed when scheduled for a 500-ml volume expansion. The 30-min ECG prior to volume expansion was analyzed for the occurrence of extrasystoles. Classification variables were defined as the change in a variable (e.g., systolic blood pressure or pre-ejection period) from the median of ten preceding sinus beats to extrasystolic post-ectopic beat. A stroke volume increase >â10% following volume expansion defined fluid responsiveness. Results: Twenty-six patients were included. The change in systolic blood pressure predicted fluid responsiveness with receiver operating characteristic (ROC) area 0.79 (CI [0.52:1.00]), specificity 100%, sensitivity 67%, positive predictive value 100%, and negative predictive value 91% (threshold: 5%). The change in pre-ejection period predicted fluid responsiveness with ROC area 0.74 (CI [0.53:0.94]), specificity 78%, sensitivity 67%, positive predictive value 50%, and negative predictive value 88% (threshold 7.5 ms). Conclusions: Based on standard critical care monitoring, analysis of the extrasystolic post-ectopic beat predicts fluid responsiveness in critical care patients with good accuracy. The presented results are considered preliminary proof-of-concept results, and further validation is needed to confirm these preliminary findings
Age-related changes in expression of the neural cell adhesion molecule in skeletal muscle: a comparative study of newborn, adult and aged rats
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