4 research outputs found
Kiemelt természetvédelmi jelentőségű hazai vizicsigák (Mollusca: Gastropoda) konzerváció-genetikai, molekuláris filogenetikai és filogeográfiai vizsgálata = Conservation genetics, molecular phylogeny and phylogeography of freshwater snails (Mollusca: Gastropoda) of high conservation concern in Hungary
A projekt célja endemikus illetve konzervációbiológiai szempontból jelentős puhatestű fajokkal kapcsolatos rendszertani, filogenetikai illetve filogeográfiai problémák tisztázása volt. Azt reméltük, hogy ezen kérdések tisztázása az alapkutatás jellegén túl, segíti az érintett fajokkal kapcsolatos megalapozott környezetvédelmi döntéshozatalt. A projekt főbb eredményei: Nukleáris és mitokondriális génszakaszok egyöntetűen azt igazolják, a Theodoxus prevostianus – Theodoxus danubialis fajcsoport törzsfája parafiletikus, feltehetően a mai Theodoxus danubialis a pleisztocénban élt folyólakó Theodoxus prevostianus egyik génvonalából rapid evolucióval létrejött fiatal faj. Kimutattuk, hogy meglepő módon a mitokondriális COI gén alig variál a Theodoxus transversalis mára még fennmaradt populációi között. Feltételezésünk szerint a mitokondriális variabilitás hiánya nem a közelmúltbeli kihalások következménye, hanem egy kora holocén-, vagy késő pleisztocénkori palacknyak hatásé. Mitokondriális COI génszakasz alapján közel 5% os intraspecifikus variabilitást és mélyen szétvált génvonal jelenlétét mutatuk ki a Bythinella pannonica fajon belül, amelyeknek a térbeli eloszlása alapján összetett elterjedéstörténetre következtetünk. Mind az ivarszervek morfológiája, mind a vizsgált molekuláris markerek azt igazolják, hogy a Bythinella molcsanyi önálló faj és ezért nagy konzervációbiológiai jelentősége van. Ezzel szemben kimutattuk, hogy a Bythinella hungarica a széles elterjedésű Bythinella austriaca faj szinonímja. | The aim of this project was to clarify taxonomical, filogenetic and filogeographic questions which are associated to endemic mollusc species or other mollusc species of conservation concern. We hoped that clarifying these problems will help to make effective conservation decisions. The main goals are the following: Nuclear and mitochondrial sequences concordantly show that the phylogeny of the Theodoxus prevostianus – Theodoxus danubialis species group is paraphyletic. Recent Theodoxus danubialis is probably a young species, which has evolved from a lineage of the fluvial T. prevostianus in the Pleistocene. We have found unexpectedly low mitochondrial COI variability in the remnant populations of Theodoxus transversalis. This phenomenon seems to be the consequence of a historical bottleneck. In our view, the whole Holocene range has been colonized by descendants of a bottlenecked population. Based on mitochondrial COI sequence, nearly 5% intraspecific variability and two deeply diverged lineages were found in the Bythinella pannonica species. Its spatial distribution infers a complex distribution history. Both genital morphology and mitochondrial phylogeny (COI) proved the monophyly and the distinct species status of Bythinella molcsanyi, which is therefore, a narrow range species of high conservation concern. In contrast, Bythinella hungarica was proved to be a synonym of the widespread Bythinella austriaca
The paternal genetic legacy of Hungarian-speaking Rétköz (Hungary) and Váh valley (Slovakia) populations
One hundred and six Rétköz and 48 Váh valley samples were collected from the contact zones of Hungarian-Slovakian territories and were genotyped for Y-chromosomal haplotypes and haplogroups. The results were compared with contemporary and archaic data from published sources. The genetic composition of the Rétköz population from Hungary and the Váh valley population from Slovakia indicates different histories. In the Rétköz population, the paternal lineages that were also found in the Hungarian Conquerors, such as R1a-Z93, N-M46, Q-M242, and R1b-L23, were better preserved. These haplogroups occurred in 10% of the population. The population of the Váh valley, however, is characterized by the complete absence of these haplogroups. Our study did not detect a genetic link between the Váh valley population and the Hungarian Conquerors; the genetic composition of the Váh valley population is similar to that of the surrounding Indo-European populations. The Hungarian Rétköz males shared common haplotypes with ancient Xiongnu, ancient Avar, Caucasian Avar, Abkhazian, Balkarian, and Circassian males within haplogroups R1a-Z93, N1c-M46, and R1b-L23, indicating a common genetic footprint. Another difference between the two studied Hungarian populations can be concluded from the Fst-based MDS plot. The Váh valley, in the western part of the Hungarian-Slovakian contact zone, is genetically closer to the Western Europeans. In contrast, Rétköz is in the eastern part of that zone and therefore closer to the Eastern Europeans
Concordance of the spectral properties of dorsal wing scales with the phylogeographic structure of European male Polyommatus icarus butterflies
Abstract The males of more than 80% of the Lycaenidae species belonging to the tribe Polyommatini exhibit structural coloration on their dorsal wing surfaces. These colors have a role in reinforcement in prezygotic reproductive isolation. The species-specific colors are produced by the cellular self-assembly of chitin/air nanocomposites. The spectral position of the reflectance maximum of such photonic nanoarchitectures depends on the nanoscale geometric dimensions of the elements building up the nanostructure. Previous work showed that the coloration of male Polyommatus icarus butterflies in the Western and Eastern Palearctic exhibits a characteristic spectral difference (20 nm). We investigated the coloration and the de novo developed DNA microsatellites of 80 P. icarus specimens from Europe from four sampling locations, spanning a distance of 1621 km. Remarkably good concordance was found between the spectral properties of the blue sexual signaling color (coincident within 5 nm) and the population genetic structure as revealed by 10 microsatellites for the P. icarus species
European rodent on the edge: status and distribution of the Vojvodina blind mole rat
Recent research of blind mole rats of the species complex Nannospalax (superspecies leucodon) identified a small and fragmented population of these rodents on both sides of the Hungarian-Serbian border. Cytogenetic investigations proved that this population karyologically identical with the Vojvodina blind mole rat described earlier as Nannospalax (leucodon) montanosyrmiensis. Based on cytochrome b gene sequences obtained from three specimens originating from separate locations, these blind mole rats form a discrete phylogenetic clade which, with a difference of about 10%, is well separated from other blind mole rat taxa inhabiting the Carpathian Basin. The taxon has only two extant populations that are 150 km apart from each other. The combined occupied area is estimated to be less than 10 km(2), and the total estimated number of individuals is less than 300. These two remaining populations are heavily fragmented and under imminent threat by the establishment of tree plantations, small-scale and agro-industrial farms and land development. The situation is further aggravated by the fact that 80% of the individuals inhabit unprotected areas. A study of the landscape history of the wider area surrounding one of the populations - based on military maps spanning over the last 200 years - has shown a drastic decrease in the extent and quality of potential habitats. Based on our present knowledge, the Vojvodina blind mole rat is one of the most seriously threatened, rarest mammal in Europe, the remaining population of which can be wiped out within years unless immediate conservation action is taken. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/2193-1801-2-2) contains supplementary material, which is available to authorized users