Az elektronikus írásbeliség és problémái

In situ plan. (PDF 1566 kb

MOESM1 of Boosting of enzymatic softwood saccharification by fungal GH5 and GH26 endomannanases

Additional file 1: Table S1 and S2. Ratings from one-way ANOVA analyses of initial reaction rates by endomannanases on pure mannans (Fig. 2). A one-way ANOVA analysis was made for all endomannanases on each substrate (top, Table S1) and for each enzyme across all substrates (bottom, Table S2). Ratings are assigned with a 95 % confidence interval with the Tukey–Kramer method in SASjmp. Please consult the manuscript for enzyme abbreviations. Table S3. Ratings from one-way ANOVA analyses of sugar release during softwood saccharification at 30 °C (Fig. 3 and Additional file 1: Figure S2). A one-way ANOVA analysis was made for each sugar at each time point. Ratings are assigned with a 95 % confidence interval with the Tukey–Kramer method in SASjmp. Please consult the manuscript for enzyme abbreviations. Table S4. Ratings from one-way ANOVA analyses of glucose release during softwood saccharification at 30 and 50 °C (Fig. 4). A one-way ANOVA analysis was made for each temperature at each time point. Ratings are assigned with a 95 % confidence interval with the Tukey–Kramer method in SASjmp. Please consult the manuscript for enzyme abbreviations. Figure S1. Stability of endomannanases at 30 °C. The relative activity (%) was determined on locust bean gum at 30 °C, pH 5 and are given as mean values ± SD (n=3). Please consult the manuscript for enzyme abbreviations. Figure S2. Softwood saccharification. Endomannanases or BSA were added in equal molar amounts on top of Cellic ® CTec3 plus an A. niger GH2 β-mannosidase (BM2). Samples were taken after 24, 48 and 144 h saccharification at 30 °C. Glucose (g/l, light grey), mannose (dark grey) and xylose (black) yields are given as mean values ± SD (n=3). One-way ANOVA analyses can be seen in Additional file 1: Table S3. Figure S3. SDS-PAGE gels of the purified enzymes. The protein concentration in the samples was 0.5 mg/ml. Prior to gel loading, samples were diluted 1:1 with loading mix. Loading mix was prepared as a 9:1 mix of Novex ® Tris-Glycine SDS Sample Buffer (2X) (Life Technologies) and Nupage ® Sample Reducing Agent (10X) (Life Technologies). Please consult the manuscript for enzyme abbreviations. Samples with NdesMan26A and MtheMan26A both contain molecules with and without the CBM35

MOESM4 of Removal of glucuronic acid from xylan is a strategy to improve the conversion of plant biomass to sugars for bioenergy

Additional file 4: Table S2. Gene Bank and OneKP transcript catalogue numbers. Those transcripts encode conifer GUX enzymes used to construct the maximum likelihood phylogeny presented in Fig. 2a