147 research outputs found
A sensitive tissue factor activity assay determined by an optimized thrombin generation method
Reply to a letter from Jackson J et al: Effect of pH on thrombin activity measured by calibrated automated thrombinography
The interrelationship between pregnancy, venous thromboembolism, and thyroid disease: a hypothesis-generating review
Unrecognized preanalytical problem with the spectrophotometric analysis of cerebrospinal fluid for xanthochromia
Thrombin generation measured on ST Genesia, a new platform in the coagulation routine lab: Assessment of analytical and between-subject variation
Background: The thrombin generation (TG) assay, which measures global coagulation, has mainly been used as a research tool to investigate thrombotic and bleeding disorders. Recently, Diagnostica Stago launched the ST Genesia, a fully automated system to perform âroutine versionâ of this assay. The objectives of this study were to evaluate the imprecision compared with the previous method, Thrombinoscope CAT, and to establish reference intervals. Methods: Thrombin generation was measured in platelet-poor citrated plasma from 20 normal controls (fresh and after freezing at â80°C up to 12â13 weeks) on CAT and ST Genesia in duplicate to estimate the total variation, and within and between variations. The reference intervals were estimated nonparametrically in 30 men, 30 women taking combined oral contraceptives (COCs), and 30 women not taking COCs. These were sampled in both Vacutainer and Monovette tubes (i.e., tubes with a high and minimal contact activation, respectively). Results: Freezing had minimal effects. Imprecision was comparable between the ST Genesia and CAT, with a strong correlation between the two methods. TG was higher when sampled in Vacutainer than in Monovette. We observed a distinct difference between women taking and not taking COCs, whereas men and women not taking COC were quite similar. Conclusions: Thrombin generation on ST Genesia showed an analytical variation similar to that of CAT. The results depended on the type of sample tubes; thus, reference intervals must be established for the collection tubes used in each laboratory. Furthermore, a considerable difference was observed between women using and not using COCs
Biophysical Characterization of a dysfunctional antithrombin variant "Antithrombin III Aalborg
Thrombocytopenia with acute ischemic stroke and bleeding in a patient newly vaccinated with an adenoviral vector-based COVID-19 vaccine
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