Potentials to differentiate milk composition by different feeding strategies

To investigate the effect of the dietary intake of the cow on milk composition, bulk-tank milk was collected on 5 occasions from conventional (n = 15) and organic (n = 10) farms in Denmark and on 4 occasions from low-input nonorganic farms in the United Kingdom, along with management and production parameters. Production of milk based on feeding a high intake of cereals, pasture, and grass silage resulted in milk with a high concentration of α-linolenic acid (9.4 ± 0.2 mg/ kg of fatty acids), polyunsaturated fatty acids (3.66 ± 0.07 mg/kg of fatty acids), and natural stereoisomer of α-tocopherol (RRR-α-tocopherol, 18.6 ± 0.5 mg/kg of milk fat). A milk production system using a high proportion of maize silage, by-products, and commercial concentrate mix was associated with milk with high concentrations of linoleic acid (LA; 19.7 ± 0.4 g/kg of fatty acids), monounsaturated fatty acids (27.5 ± 0.3 mg/kg of fatty acids), and a high ratio between LA and α-linolenic acid (4.7 ± 0.2). Comparing these 2 production systems with a very extensive nonorganic milk production system relying on pasture as almost the sole feed (95 ± 4% dry matter intake), it was found that the concentrations of conjugated LA (cis-9,trans-11; 17.5 ± 0.7 g/kg of fatty acids), trans-11-vaccenic acid (37 ± 2 g/kg of fatty acids), and monounsaturated fatty acids (30.4 ± 0.6 g/kg of fatty acids) were higher in the extensively produced milk together with the concentration of antioxidants; total α-tocopherol (32.0 ± 0.8 mg/kg of milk fat), RRR-α-tocopherol (30.2 ± 0.8 mg/kg of milk fat), and β-carotene (9.3 ± 0.5 mg/kg of milk fat) compared with the organic and conventional milk. Moreover, the concentration of LA (9.2 ± 0.7 g/kg of fatty acids) in milk from the extensive milk production system was found to approach the recommended unity ratio between n-6 and n-3, although extensive milk production also resulted in a lower daily milk yield

Tolerance and metabolic response of <i>Pseudomonas taiwanensis</i> VLB120 towards biomass hydrolysate-derived inhibitors

Abstract Background Bio-conversion of lignocellulosic biomass to high-value products offers numerous benefits; however, its development is hampered by chemical inhibitors generated during the pretreatment process. A better understanding of how microbes naturally respond to those inhibitors is valuable in the process of designing microorganisms with improved tolerance. Pseudomonas taiwanensis VLB120 is a natively tolerant strain that utilizes a wide range of carbon sources including pentose and hexose sugars. To this end, we investigated the tolerance and metabolic response of P. taiwanensis VLB120 towards biomass hydrolysate-derived inhibitors including organic acids (acetic acid, formic acid, and levulinic acid), furans (furfural, 5-hydroxymethylfurfural), and phenols (vanillin). Results The inhibitory effect of the tested compounds varied with respect to lag phase, specific growth rate, and biomass yield compared to the control cultures grown under the same conditions without addition of inhibitors. However, P. taiwanensis was able to oxidize vanillin and furfural to vanillic acid and 2-furoic acid, respectively. Vanillic acid was further metabolized, whereas 2-furoic acid was secreted outside the cells and remained in the fermentation broth without further conversion. Acetic acid and formic acid were completely consumed from the fermentation broth, while concentration of levulinic acid remained constant throughout the fermentation process. Analysis of free intracellular metabolites revealed varying levels when P. taiwanensis VLB120 was exposed to inhibitory compounds. This resulted in increased levels of ATP to export inhibitors from the cell and NADPH/NADP ratio that provides reducing power to deal with the oxidative stress caused by the inhibitors. Thus, adequate supply of these metabolites is essential for the survival and reproduction of P. taiwanensis in the presence of biomass-derived inhibitors. Conclusions In this study, the tolerance and metabolic response of P. taiwanensis VLB120 to biomass hydrolysate-derived inhibitors was investigated. P. taiwanensis VLB120 showed high tolerance towards biomass hydrolysate-derived inhibitors compared to most wild-type microbes reported in the literature. It adopts different resistance mechanisms, including detoxification, efflux, and repair, which require additional energy and resources. Thus, targeting redox and energy metabolism in strain engineering may be a successful strategy to overcome inhibition during biomass hydrolysate conversion and lead to development of more robust strains

Conductance of a quantum point contact based on spin-density-functional theory

We present full quantum mechanical conductance calculations of a quantum point contact (QPC) performed in the framework of the density functional theory (DFT) in the local spin-density approximation (LDA). We show that a spin-degeneracy of the conductance channels is lifted and the total conductance exhibits a broad plateau-like feature at 0.5*2e^{2}/h. The lifting of the spin-degeneracy is a generic feature of all studied QPC structures (both very short and very long ones; with the lengths in the range 40<l<500 nm). The calculated conductance also shows a hysteresis for forward- and backward sweeps of the gate voltage. These features in the conductance can be traced to the formation of weakly coupled quasi-bound states (magnetic impurities) inside the QPC (also predicted in previous DFT-based studies). A comparison of obtained results with the experimental data shows however, that while the spin-DFT based "first-principle" calculations exhibits the spin polarization in the QPC, the calculated conductance clearly does not reproduce the 0.7 anomaly observed in almost all QPCs of various geometries. We critically examine major features of the standard DFT-based approach to the conductance calculations and argue that its inability to reproduce the 0.7 anomaly might be related to the infamous derivative discontinuity problem of the DFT leading to spurious self-interaction errors not corrected in the standard LDA. Our results indicate that the formation of the magnetic impurities in the QPC might be an artefact of the LDA when localization of charge is expected to occur. We thus argue that an accurate description of the QPC structure would require approaches that go beyond the standard DFT+LDA schemes.Comment: 9 pages, 5 figure

Ballistic reflection at a side-gate in a superconductor-semiconductor-superconductor structure

We have fabricated a sub-micron-sized structure consisting of an InAs-based 2DEG, two narrow Nb leads and a gate, where the indirect ballistic transport between the non-oppositely superconducting contacts can be controlled by the voltage applied to the gate. This new kind of tuneable junction can be used for applications and allows several fundamental questions related to the transport mechanism to be studied. First results of experiments carried out in this respect are presented.Comment: 6 pages, 4 eps-figure

Far infrared CO and H2_2O emission in intermediate-mass protostars

Intermediate-mass young stellar objects (YSOs) provide a link to understand how feedback from shocks and UV radiation scales from low to high-mass star forming regions. Aims: Our aim is to analyze excitation of CO and H$_2$O in deeply-embedded intermediate-mass YSOs and compare with low-mass and high-mass YSOs. Methods: Herschel/PACS spectral maps are analyzed for 6 YSOs with bolometric luminosities of $L_\mathrm{bol}\sim10^2 - 10^3$ $L_\odot$. The maps cover spatial scales of $\sim 10^4$ AU in several CO and H$_2$O lines located in the $\sim55-210$ $\mu$m range. Results: Rotational diagrams of CO show two temperature components at $T_\mathrm{rot}\sim320$ K and $T_\mathrm{rot}\sim700-800$ K, comparable to low- and high-mass protostars probed at similar spatial scales. The diagrams for H$_2$O show a single component at $T_\mathrm{rot}\sim130$ K, as seen in low-mass protostars, and about $100$ K lower than in high-mass protostars. Since the uncertainties in $T_\mathrm{rot}$ are of the same order as the difference between the intermediate and high-mass protostars, we cannot conclude whether the change in rotational temperature occurs at a specific luminosity, or whether the change is more gradual from low- to high-mass YSOs. Conclusions: Molecular excitation in intermediate-mass protostars is comparable to the central $10^{3}$ AU of low-mass protostars and consistent within the uncertainties with the high-mass protostars probed at $3\cdot10^{3}$ AU scales, suggesting similar shock conditions in all those sources.Comment: Accepted to Astronomy & Astrophysics. 4 pages, 5 figures, 3 table