BEHAVIORAL INTERVENTION FOR EATING AND EXERCISE CHALLENGES IN CHILDREN WITH AUTISM SPECTRUM DISORDER

Autism Spectrum Disorder is a neurodevelopmental disability characterized by deficits in social-emotional reciprocity, deficits in nonverbal communicative behaviors relied on for social interactions, deficits in developing, maintaining, and understanding relationships, as well as restricted and repetitive patterns of interest and behavior, and sensitivity to certain sensory inputs. Individuals with Autism Spectrum Disorder are at an increased risk for multiple medical health concerns when compared to typically developing individuals, including overweight and obesity. Childhood obesity is a significant public health concern in the U.S. While many efforts have been made to prevent childhood obesity, few have been designed to align with the unique challenges faced by individuals on the autism spectrum. The present program development model includes combined weekly in-clinic therapy sessions and daily participation in online activities for parent(s) of autistic children as well as the child who is on the autism spectrum. Using the “5-2-1-0 Healthy Numbers for Kentucky Kids” initiative as a basis for behavior goals, the present program proposes implementation of interventions with empirical support for treating this population. Importantly, this intervention is meant to be applicable to children with autism across the nation, not only those residing in Kentucky. While the original initiative (5-2-1-0 Healthy Numbers for Kentucky Kids) on which this intervention is based was created for Kentucky children, childhood obesity is a national concern (Vorkoper, Artega, Berrigan, Bialy, Bremer, Cotton, & Anand, 2021)

Airborne Multidrug-Resistant Bacteria Isolated from a Concentrated Swine Feeding Operation

The use of nontherapeutic levels of antibiotics in swine production can select for antibiotic resistance in commensal and pathogenic bacteria in swine. As a result, retail pork products, as well as surface and groundwaters contaminated with swine waste, have been shown to be sources of human exposure to antibiotic-resistant bacteria. However, it is unclear whether the air within swine operations also serves as a source of exposure to antibiotic-resistant bacterial pathogens. To investigate this issue, we sampled the air within a concentrated swine feeding operation with an all-glass impinger. Samples were analyzed using a method for the isolation of Enterococcus. A total of 137 presumptive Enterococcus isolates were identified to species level using standard biochemical tests and analyzed for resistance to erythromycin, clindamycin, virginiamycin, tetracycline, and vancomycin using the agar dilution method. Thirty-four percent of the isolates were confirmed as Enterococcus, 32% were identified as coagulase-negative staphylococci, and 33% were identified as viridans group streptococci. Regardless of bacterial species, 98% of the isolates expressed high-level resistance to at least two antibiotics commonly used in swine production. None of the isolates were resistant to vancomycin, an antibiotic that has never been approved for use in livestock in the United States. In conclusion, high-level multidrug-resistant Enterococcus, coagulase-negative staphylococci, and viridans group streptococci were detected in the air of a concentrated swine feeding operation. These findings suggest that the inhalation of air from these facilities may serve as an exposure pathway for the transfer of multidrug-resistant bacterial pathogens from swine to humans

Improving Pathways to Transit for Persons with Disabilities

Persons with disabilities can achieve a greater degree of freedom when they have full access to a variety of transit modes, but this can only be achieved when the pathways to transit – the infrastructure and conditions in the built environment – allow full access to transit stops, stations, and vehicles. Since passage of the Americans with Disabilities Act (ADA) in 1990, many transit agencies and governmental jurisdictions have made significant progress in this area. Policy initiatives, incremental enhancements, modifications, and other measures undertaken by transit agencies and their partners have significantly improved access to transit for persons with disabilities, others who rely on public transportation, and individuals who chose to utilize these services. This research study explores, through case study work, efforts that have been effective in improving pathways to transit. Interviews and site visits were conducted with five transit agencies, along with their partners, that are actively engaged in improving pathways to connect transit consumers – particularly people with disabilities – with transit stations and stops. These agencies are: Broward County Transit (Broward County, FL), Memphis Area Transit Authority (Memphis, TN), NJ TRANSIT (Newark and New Brunswick, NJ), Tri-County Metropolitan Transportation District of Oregon (Portland, OR), and Link Transit (Wenatchee, WA). Promising practices and/or lessons were identified through the case study analysis; these should be considered by any transit agency seeking to create improved access to its services for persons with disabilities

Contamination of Common Area and Rehabilitation Gym Environment with Multidrug‐Resistant Organisms

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/154343/1/jgs16284.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/154343/2/jgs16284_am.pd

ResearchCOVID‐19 Preparedness in Michigan Nursing Homes

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/155466/1/jgs16490_am.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/155466/2/jgs16490.pd

Understanding short-term transmission dynamics of methicillin-resistant Staphylococcus aureus in the patient room.

OBJECTIVE Little is known about the short-term dynamics of methicillin-resistant Staphylococcus aureus (MRSA) transmission between patients and their immediate environment. We conducted a real-life microbiological evaluation of environmental MRSA contamination in hospital rooms in relation to recent patient activity. DESIGN Observational pilot study. SETTING Two hospitals, hospital 1 in Zurich, Switzerland, and hospital 2 in Ann Arbor, Michigan, United States. PATIENTS Inpatients with MRSA colonization or infection. METHODS At baseline, the groin, axilla, nares, dominant hands of 10 patients and 6 environmental high-touch surfaces in their rooms were sampled. Cultures were then taken of the patient hand and high-touch surfaces 3 more times at 90-minute intervals. After each swabbing, patients' hands and surfaces were disinfected. Patient activity was assessed by interviews at hospital 1 and analysis of video footage at hospital 2. A contamination pressure score was created by multiplying the number of colonized body sites with the activity level of the patient. RESULTS In total, 10 patients colonized and/or infected with MRSA were enrolled; 40 hand samples and 240 environmental samples were collected. At baseline, 30% of hands and 20% of high-touch surfaces yielded MRSA. At follow-up intervals, 8 (27%) of 30 patient hands, and 10 (6%) of 180 of environmental sites were positive. Activity of the patient explained 7 of 10 environmental contaminations. Patients with higher contamination pressure score showed a trend toward higher environmental contamination. CONCLUSION Environmental MRSA contamination in patient rooms was highly dynamic and was likely driven by the patient's MRSA body colonization pattern and the patient activity

Prefrontal Neurons Encode Actions and Outcomes in Conjunction with Spatial Location in Rats Performing a Dynamic Delayed Non-Match to Position Task

To respond adaptively to change organisms must utilize information about recent events and environmental context to select actions that are likely to produce favorable outcomes. We developed a dynamic delayed nonmatching to position task to study the influence of spatial context on event-related activity of medial prefrontal cortex neurons during reinforcement-guided decision-making. We found neurons with responses related to preparation, movement, lever press responses, reinforcement, and memory delays. Combined event-related and video tracking analyses revealed variability in spatial tuning of neurons with similar event-related activity. While all correlated neurons exhibited spatial tuning broadly consistent with relevant task events, for instance reinforcement-related activity concentrated in locations where reinforcement was delivered, some had elevated activity in more specific locations, for instance reinforcement-related activity in one of several locations where reinforcement was delivered. Timing analyses revealed a limited set of distinct response types with activity time-locked to critical behavioral events that represent the temporal organization of dDNMTP trials. Our results suggest that reinforcement-guided decision-making emerges from discrete populations of medial prefrontal neurons that encode information related to planned or ongoing movements and actions and anticipated or actual action-outcomes in conjunction with information about spatial context

Coordination of myeloid differentiation with reduced cell cycle progression by PU.1 Induction of microRNAs targeting cell cycle regulators and lipid anabolism

During macrophage development, myeloid progenitor cells undergo terminal differentiation coordinated with reduced cell cycle progression. Differentiation of macrophages from myeloid progenitors is accompanied by increased expression of the E26 transformation-specific transcription factor PU.1. Reduced PU.1 expression leads to increased proliferation and impaired differentiation of myeloid progenitor cells. It is not understood how PU.1 coordinates macrophage differentiation with reduced cell cycle progression. In this study, we utilized cultured PU.1- inducible myeloid cells to perform genome-wide chromatin immunoprecipitation sequencing (ChIP-seq) analysis coupled with gene expression analysis to determine targets of PU.1 that may be involved in regulating cell cycle progression. We found that genes encoding cell cycle regulators and enzymes involved in lipid anabolism were directly and inducibly bound by PU.1 although their steady-state mRNA transcript levels were reduced. Inhibition of lipid anabolism was sufficient to reduce cell cycle progression in these cells. Induction of PU.1 reduced expression of E2f1, an important activator of genes involved in cell cycle and lipid anabolism, indirectly through microRNA 223. Next-generation sequencing identified microRNAs validated as targeting cell cycle and lipid anabolism for downregulation. These results suggest that PU.1 coordinates cell cycle progression with differentiation through induction of microRNAs targeting cell cycle regulators and lipid anabolism

Witnessing the Birth of a Quasar

The coalescence of a supermassive black hole binary (SMBHB) is thought to be accompanied by an electromagnetic (EM) afterglow, produced by the viscous infall of the surrounding circumbinary gas disk after the merger. It has been proposed that once the merger has been detected in gravitational waves (GWs) by LISA, follow-up EM searches for this afterglow can help identify the EM counterpart of the LISA source. Here we study whether the afterglows may be sufficiently bright and numerous to be detectable in EM surveys alone. The viscous afterglow, which lasts for years to decades for SMBHBs in LISA's sensitivity window, is characterized by rapid increases in both the bolometric luminosity and in the spectral hardness of the source. If quasar activity is triggered by the same major galaxy mergers that produce SMBHBs, then the afterglow could be interpreted as a signature of the birth of a quasar. Using an idealized model for the post-merger viscous spreading of the circumbinary disk and the resulting light curve, and using the observed luminosity function of quasars as a proxy for the SMBHB merger rate, we delineate the survey requirements for identifying such birthing quasars. If circumbinary disks have a high disk surface density and viscosity, an all-sky soft X-ray survey with a sensitivity of ~<3x10^-14 erg s^-1 cm^-2 and a time resolution of ~months could identify dozens of birthing quasars with sustained brightening rates of >10%/yr. If >1% of the X-ray emission is reprocessed into optical frequencies, birthing quasars could also be identified in optical transient surveys such as the LSST. Distinguishing a birthing quasar from other variable sources may be facilitated by the monotonic hardening of its spectrum, but will likely remain challenging. This reinforces the notion that joint EM-plus-GW observations offer the best prospects for identifying the EM signatures of SMBHB mergers.Comment: 27 pages, 5 figures. Updated references. Accepted to the Astronomical Journal, 140 (2010) 642-65