162 research outputs found
Gross karyotypic and phenotypic alterations among different progenies of the candida glabrata cbs138/atcc2001 reference strain
Peer reviewedPublisher PD
The Association of Insomnia, Perceived Immune Functioning, and Irritable Bowel Syndrome Complaints
BACKGROUND: Irritable bowel syndrome (IBS) can have a significant negative impact on quality of life, mood and wellbeing. The aim of this study was to investigate the association between experiencing IBS symptoms and insomnia, and perceived health status.
METHOD: An online survey was conducted among n = 1950 Dutch university students (83.6% women). IBS was assessed with the Birmingham IBS Symptom Questionnaire, quality of life with the WHO-5 wellbeing index, and sleep outcomes with the SLEEP-50 questionnaire. Perceived immune functioning and general health were assessed using 1-item scales.
RESULTS: IBS symptom severity was significantly associated with insomnia complaints (r = 0.32, p = 0.0001), sleep quality (r = -0.21, p = 0.0001), sleep onset latency (r = 0.11, p = 0.0001) and the number of nightly awakenings (r = 0.24, p = 0.0001). Total sleep time was not significantly associated with IBS symptom severity. Significant correlations were also found between IBS symptom severity and perceived general health (r = -0.30, p = 0.0001), perceived immune functioning (r= -0.25, p = 0.0001), and quality of life (r = -0.24, p = 0.0001).
CONCLUSIONS: Experiencing IBS complaints is associated with reduced perceived immune functioning, a poorer perception of general health, and sleep disturbances. These effects are reflected in a significantly lower reported quality of life in subjects with more IBS and/or sleep complaints
ΠΠ°Π·ΠΈΡΠΈΠΊΠ°ΡΠΈΡ ΠΏΡΠΎΠΌΡΡΠ»Π΅Π½Π½ΡΡ ΠΎΡΡ ΠΎΠ΄ΠΎΠ² Π½Π΅ΠΏΡΠ΅ΡΡΠ²Π½ΡΠΌ Π»Π°Π·Π΅ΡΠ½ΡΠΌ ΠΈΠ·Π»ΡΡΠ΅Π½ΠΈΠ΅ΠΌ
Liposome-encapsulated corticosteroids have shown to exert strong beneficial effects in inflammatory diseases, such as arthritis and cancer. To extend the clinical applicability of these potent nanomedicines, the therapeutic effect of dexamethasone phosphate loaded long-circulating liposomes (LCL-DXP) was evaluated in animal models of multiple sclerosis (MS) and Crohn's disease (CD). In mice with experimental autoimmune encephalitis (EAE), a model for MS, treatment with LCL-DXP, but not free DXP, resulted in a decrease in disease activity when compared to PBS treated mice. In contrast, in mice with chronic DSS-induced colitis, a model for CD, treatment with LCL-DXP did not induce an improvement, but in fact worsened the fecal blood loss after treatment, indicating an aggravation of the disease. It is hypothesized that modulation of macrophage polarization towards a M2 phenotype underlies the efficacy of corticosteroid-based drug delivery systems, which is supported by the presented data. On the one hand, M1 polarized macrophages are part of the pathogenesis of MS; the modulation to M2-polarization by LCL-DXP is therefore beneficial. On the other hand, M1-polarized intestinal macrophages fulfill a protective and inflammation-suppressing role in intestinal homeostasis; changing their phenotype to M2 causes reduced protection to invading microorganisms, leading to a more severe intestinal inflammation. These findings therefore indicate that the interplay between the specific phenotype of macrophages and the specific inflammatory context of the inflammatory disease in question may be an important determining factor in the therapeutic applicability of liposomal corticosteroids in inflammatory disease
ΠΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡΠΈ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΡ ΠΊΡΠ΅Π½ΠΎΡΠ΅Π»Π΅Π·Π΅Π½ΠΊΠΈ Π² ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΏΡΠ°ΠΊΡΠΈΠΊΠ΅
Π Π°ΡΡΠΌΠΎΡΡΠ΅Π½Π° ΠΏΡΠΎΠ±Π»Π΅ΠΌΠ° ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΡ ΠΊΡΠ΅Π½ΠΎΡΠ΅Π»Π΅Π·Π΅Π½ΠΊΠΈ Π² ΠΊΠ»ΠΈΠ½ΠΈΠΊΠ΅. ΠΠΎΠΊΠ°Π·Π°Π½Π° ΡΠ΅Π»Π΅ΡΠΎΠΎΠ±ΡΠ°Π·Π½ΠΎΡΡΡ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΡ ΠΊΡΠΈΠΎΠ±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
ΡΠ΅Ρ
Π½ΠΎΠ»ΠΎΠ³ΠΈΠΉ Π΄Π»Ρ ΠΏΠΎΠ»ΡΡΠ΅Π½ΠΈΡ Π±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈ Π°ΠΊΡΠΈΠ²Π½ΡΡ
ΡΠΊΡΡΡΠ°ΠΊΡΠΎΠ² ΠΈΠ· ΡΡΠ°Π³ΠΌΠ΅Π½ΡΠΎΠ² ΠΊΡΠ΅Π½ΠΎΡΠ΅Π»Π΅Π·Π΅Π½ΠΊΠΈ. Π Π°Π·ΡΠ°Π±ΠΎΡΠ°Π½ ΡΠ°ΠΊΠΎΠΉ ΡΠΊΡΡΡΠ°ΠΊΡ, ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½Ρ Π΅Π³ΠΎ Π±ΠΈΠΎΡΠ°ΡΠΌΠ°ΡΠ΅Π²ΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΡΠ²ΠΎΠΉΡΡΠ²Π° ΠΈ ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠ°Ρ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΠΏΡΠΈ ΡΠ½Π΄ΠΎΠ±ΡΠΎΠ½Ρ
ΠΈΠ°Π»ΡΠ½ΠΎΠΌ Π²Π²Π΅Π΄Π΅Π½ΠΈΠΈ Π±ΠΎΠ»ΡΠ½ΡΠΌ Ρ Π°Π±ΡΡΠ΅ΡΡΠ°ΠΌΠΈ Π»Π΅Π³ΠΊΠΈΡ
.The problem of xenospleen use in clinical practice is discussed. Reasonability of application of cryobiological technologies to obtain biologically active extracts from xenospleen fragments is shown. The extract has been developed, its biopharmaceutical properties and clinical efficacy at endobronchial administration in patients with lung abscess is shown
ΠΡΠΎΠ±Π΅Π½Π½ΠΎΡΡΠΈ ΠΏΡΠΎΡΠ΅ΡΡΠ° ΡΡΠ΅ΡΠΈΠ½ΠΎΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΡ Π² ΠΌΠ°ΡΡΠΈΠ²Π΅ ΠΏΡΠΈ ΡΠΏΡΠ°Π²Π»Π΅Π½ΠΈΠΈ Π΅Π³ΠΎ Π³Π°Π·ΠΎΠ΄ΠΈΠ½Π°ΠΌΠΈΠΊΠΎΠΉ
ΠΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ ΠΏΡΠΎΡΠ΅ΡΡ ΡΠ΄Π΅ΡΠΆΠΈΠ²Π°Π½ΠΈΡ ΠΏΠ΅ΡΠ΅Ρ
ΠΎΠ΄Π° ΡΠ³Π»Ρ ΠΈΠ· ΠΏΠΎΡΠ΅Π½ΡΠΈΠ°Π»ΡΠ½ΠΎ ΡΡΡΠΎΠΉΡΠΈΠ²ΠΎΠ³ΠΎ ΡΠΎΡΡΠΎΡΠ½ΠΈΡ
Π² ΡΡΠ°Π΄ΠΈΡ Π±ΡΡΠ½ΠΎΠ³ΠΎ ΡΠ°Π·ΡΡΡΠ΅Π½ΠΈΡ. ΠΠΉ, ΠΊΠ°ΠΊ ΠΏΡΠ°Π²ΠΈΠ»ΠΎ, ΠΏΡΠ΅Π΄ΡΠ΅ΡΡΠ²ΡΠ΅Ρ Π½Π΅ΠΊΠΎΡΠΎΡΡΠΉ ΠΏΡΠΎΠΌΠ΅ΠΆΡΡΠΎΠΊ Π²ΡΠ΅ΠΌΠ΅Π½ΠΈ ΠΎΡΠ½ΠΎΡΠΈΡΠ΅Π»ΡΠ½ΠΎΠ³ΠΎ Π·Π°ΡΠΈΡΡΡ. ΠΡΠΎΠ±Π΅Π½Π½ΠΎ Π²Π°ΠΆΠ½ΠΎ ΡΠ»Π°Π²Π»ΠΈΠ²Π°ΡΡ ΡΡΠΎΡ ΠΌΠΎΠΌΠ΅Π½Ρ ΡΡΠ΅Π΄ΠΈ ΠΌΠ°ΡΡΡ ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ
ΠΎΡΠΊΠ»ΠΈΠΊΠΎΠ² ΠΌΠ°ΡΡΠΈΠ²Π° Π½Π° Π²Π΅Π΄Π΅Π½ΠΈΠ΅ Π³ΠΎΡΠ½ΡΡ
ΡΠ°Π±ΠΎΡ. ΠΠ΄Π½ΠΈΠΌ ΠΈΠ· Π²Π°ΡΠΈΠ°Π½ΡΠΎΠ² ΡΠΏΡΠ°Π²Π»Π΅Π½ΠΈΡ ΡΠ°Π·Π²ΠΈΡΠΈΠ΅ΠΌ ΠΈ ΡΠ΅Π»Π°ΠΊΡΠ°ΡΠΈΠ΅ΠΉ ΡΠΈΡΡΠ΅ΠΌΡ ΡΡΠ΅ΡΠΈΠ½ ΠΌΠΎΠΆΠ΅Ρ ΡΠ»ΡΠΆΠΈΡΡ ΡΠΈΠ·ΠΈΠΊΠΎ-Ρ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠ°Ρ ΠΎΠ±ΡΠ°Π±ΠΎΡΠΊΠ°.The inhibition process of coal transition from the potentially stable state in the stage of stormy destruction is investigation. As a rule, to it is preceded some interval of relative time calm. It
is especially important to catch this moment among mass of different responses of array on the
conduct of mountain works. Physical and chemical treatment can serve as one of control variants
the development and relaxation of the cracks system
Susceptibility to alcohol hangovers: Not just a matter of being resilient
Introduction: Although most drinkers have experienced a hangover the day following heavy alcohol consumption, a minority claims to be hangover resistant despite consuming the same large quantities of alcohol as those reporting alcohol hangover. The aim of the current study was to examine if susceptibility to experiencing hangovers is related to a drinker's interpretation of wellbeing and psychological assets to bounce back. Methods: A survey was conducted among 2295 Dutch students assessing their past month alcohol consumption patterns, and measuring mental resilience and wellbeing. Estimated peak blood alcohol concentration (e-pBAC) for their heaviest drinking occasion in the past month was computed for each participant. Data from participants who reported a past month hangover, i.e. hangover sensitive drinkers, were compared with hangover resistant drinkers. The analyses were conducted for (a) all participants reaching an e-pBAC β₯ 0.11% (N = 986, of which 24.6% claimed to be hangover resistant) and (b) participants reaching an e-pBAC β₯ 0.18% (N = 480, of which 16.7% claimed to be hangover resistant). Results: For both e-pBAC cut-off values, no significant differences between hangover sensitive and hangover resistant drinkers were found for mental resilience and wellbeing. Conclusion: The current findings suggest that having a hangover is not simply an expression of poor psychological coping with the next-day consequences of heavy alcohol consumption
Early-life antibiotic use and risk of attention-deficit hyperactivity disorder and autism spectrumΒ disorder:results of a discordant twinΒ study
Background Development of the gut-brain axis in early life may be disturbed by antibiotic use. It has been hypothesized that this disturbance may contribute to development of neurodevelopmental disorders, including autism spectrum disorder and attention-deficit hyperactivity disorder. We aimed to assess the association between antibiotic use in early life and the risk of developing attention-deficit hyperactivity disorder or autism spectrum disorder, while controlling for shared genetic and environmental factors in a discordant twin design. Methods We conducted a cohort study in twins (7β12βyears; 25 781 twins) from the Netherlands Twin Register (NTR) and a replication study in the Childhood and Adolescent Twin Study in Sweden (CATSS; 7946 9-year-old twins). Antibiotic use was recorded before age 2 years. Attention-deficit hyperactivity disorder and autism spectrum disorder were parent-reported in the Netherlands Twin Register and register-based in the Childhood and Adolescent Twin Study in Sweden. Results Early-life antibiotic use was associated with increased risk of attention-deficit hyperactivity disorder development [pooled odds ratio (OR) 1.10, 95% confidence interval (CI) 1.02-1.17] and autism spectrum disorder (pooled OR 1.15, 95% CI 1.06-1.25) in a case-control design. When restricting to monozygotic twin pairs discordant for the outcome, associations disappeared for both disorders in both cohorts (attention-deficit hyperactivity disorder OR 0.90, 95% CI 0.48-1.69 and OR 0.80, 95% CI 0.37-1.76, and autism spectrum disorder OR 0.66, 95% CI 0.38-1.16 and OR 0.29, 95% CI 0.02-4.50, respectively). Conclusions Our findings suggest that the association between early-life antibiotic use and risk of attention-deficit hyperactivity and autism spectrum disorder may be confounded by shared familial environment and genetics
The Combination Therapy of Dietary Galacto-Oligosaccharides With Budesonide Reduces Pulmonary Th2 Driving Mediators and Mast Cell Degranulation in a Murine Model of House Dust Mite Induced Asthma
Background: Dietary non-digestible galacto-oligosaccharides (GOS) suppress allergic responses in mice sensitized and challenged with house dust mite (HDM). Budesonide is the standard therapy for allergic asthma in humans but is not always completely effective.Aim: To compare the efficacy of budesonide or different doses of GOS alone or with a combination therapy of budesonide and GOS on HDM-allergic responses in mice.Methods:BALB/c mice were sensitized and challenged with HDM, while fed a control diet or a diet supplemented with 1 or 2.5 w/w% GOS, and either or not oropharyngeally instilled with budesonide. Systemic and local inflammatory markers, such as mucosal mast cell protease-1 (mMCP-1) in serum, pulmonary CCL17, CCL22, and IL-33 concentrations and inflammatory cell influx in the bronchoalveolar lavage fluid (BALF) were determined.Results: Budesonide or GOS alone suppressed the number of eosinophils in the BALF of HDM allergic mice whereas budesonide either or not combined with GOS lowered both eosinophil and lymphocyte numbers in the BALF of HDM-allergic mice. Both 1 w/w% and 2.5 w/w% GOS and/or budesonide suppressed serum mMCP-1 concentrations. However, budesonide nor GOS alone was capable of reducing Th2 driving chemokines CCL17, CCL22 and IL-33 protein levels in supernatants of lung homogenates of HDM allergic mice, whereas the combination therapy did. Moreover, IL-13 concentrations were only significantly suppressed in mice treated with budesonide while fed GOS. A similar tendency was observed for the frequency of GATA3+CD4+ Th2 and CD4+RORΞ³t+ Th17 cells in the lungs of the allergic mice.Conclusion: Dietary intervention using GOS may be a novel way to further improve the efficacy of anti-inflammatory drug therapy in allergic asthma by lowering Th2 driving mediators and mast cell degranulation
A healthier daily diet is associated with greater immune fitness
Introduction: The aim of this cross-sectional study was to investigate the association between a healthier diet, perceived immune fitness, and biomarkers of the immune system. Methods: N = 108 participants (31 men and 77 women), 18β30 years old, completed a questionnaire, comprising the Healthy Diet Scale (HDS) and a 1-item scale assessing perceived immune fitness. In addition, saliva samples were collected and C-reactive protein (CRP) and cytokine concentrations of interleukin (IL)β 1Ξ² and IL-8 were determined. Results: Overall, a significant correlation was found between the HDS score and perceived immune fitness (r = 0.221, p = 0.021), suggesting that a healthier diet was associated with a better immune fitness. The HSD score correlated significantly and negatively with saliva CRP concentrations (r = β0.240, p = 0.013). No significant correlations were found with other biomarkers. In women, the HDS correlated significantly with perceived immune fitness (r = 0.247, p = 0.030) and CRP levels ( r = β0.281, p = 0.014). In men, correlations with perceived immune fitness ( r = β0.219, p = 0.237) and the biomarkers were not significant. Discussion: Significant associations between attaining a healthy diet, perceived immune fitness and CRP were found. More research is needed to investigate the observed sex differences and underlying mechanisms
Classification and specific primer design for accurate detection of SARS-CoV-2 using deep learning
In this paper, deep learning is coupled with explainable artificial intelligence techniques for the discovery of representative genomic sequences in SARS-CoV-2. A convolutional neural network classifier is first trained on 553 sequences from the National Genomics Data Center repository, separating the genome of different virus strains from the Coronavirus family with 98.73% accuracy. The networkβs behavior is then analyzed, to discover sequences used by the model to identify SARS-CoV-2, ultimately uncovering sequences exclusive to it. The discovered sequences are validated on samples from the National Center for Biotechnology Information and Global Initiative on Sharing All Influenza Data repositories, and areΒ provenΒ to be able to separate SARS-CoV-2 from different virus strains with near-perfect accuracy. Next, one of the sequences is selected to generate a primer set, and tested against other state-of-the-art primer sets, obtaining competitive results. Finally, the primer is synthesized and tested on patient samples (n = 6 previously tested positive), delivering a sensitivity similar to routine diagnostic methods, and 100% specificity. The proposed methodology has a substantial added value over existing methods, as it is able to both automatically identify promising primer sets for a virus from a limited amount of data, and deliver effective results in a minimal amount of time. Considering the possibility of future pandemics, these characteristics are invaluable to promptly create specific detection methods for diagnostics
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