Extensive morphological and behavioural diversity among fourteen new and seven described species in Phytophthora Clade 10 and its evolutionary implications

During extensive surveys of global Phytophthora diversity 14 new species detected in natural ecosystems in Chile, Indonesia, USA (Louisiana), Sweden, Ukraine and Vietnam were assigned to Phytophthora major Clade 10 based on a multigene phylogeny of nine nuclear and three mitochondrial gene regions. Clade 10 now comprises three subclades. Subclades 10a and 10b contain species with nonpapillate sporangia, a range of breeding systems and a mainly soil- and waterborne lifestyle. These include the previously described P. afrocarpa, P. gallica and P. intercalaris and eight of the new species: P. ludoviciana, P. procera, P. pseudogallica, P. scandinavica, P. subarctica, P. tenuimura, P. tonkinensis and P. ukrainensis. In contrast, all species in Subclade 10c have papillate sporangia and are self-fertile (or homothallic) with an aerial lifestyle including the known P. boehmeriae, P. gondwanensis, P. kernoviae and P. morindae and the new species P. celebensis, P. chilensis, P. javanensis, P. multiglobulosa, P. pseudochilensis and P. pseudokernoviae. All new Phytophthora species differed from each other and from related species by their unique combinations of morphological characters, breeding systems, cardinal temperatures and growth rates. The biogeography and evolutionary history of Clade 10 are discussed. We propose that the three subclades originated via the early divergence of pre-Gondwanan ancestors > 175 Mya into water- and soilborne and aerially dispersed lineages and subsequently underwent multiple allopatric and sympatric radiations during their global spread

Worldwide diversity of endophytic fungi and insects associated with dormant tree twigs

International trade in plants and climate change are two of the main factors causing damaging tree pests (i.e. fungi and insects) to spread into new areas. To mitigate these risks, a large-scale assessment of tree-associated fungi and insects is needed. We present records of endophytic fungi and insects in twigs of 17 angiosperm and gymnosperm genera, from 51 locations in 32 countries worldwide. Endophytic fungi were characterized by high-throughput sequencing of 352 samples from 145 tree species in 28 countries. Insects were reared from 227 samples of 109 tree species in 18 countries and sorted into taxonomic orders and feeding guilds. Herbivorous insects were grouped into morphospecies and were identified using molecular and morphological approaches. This dataset reveals the diversity of tree-associated taxa, as it contains 12,721 fungal Amplicon Sequence Variants and 208 herbivorous insect morphospecies, sampled across broad geographic and climatic gradients and for many tree species. This dataset will facilitate applied and fundamental studies on the distribution of fungal endophytes and insects in trees

Imeretian oak and a great capricorn beetle – the problem of relations on the territory of Ajameti Managed Reserve (Republic of Georgia)

The old-growth endemic Imeretian oak [Quercus robur subsp. imeretina (Steven ex Woronow) Menitsky, 1968] is a protected relict Tertiary period tree still preserved in Ajameti Managed Reserve (Republic of Georgia). For a long time, these forests were experienced to the strong anthropogenic influence. The old-age oak trees and the development of root and stem rot have promoted the settlement of great capricorn beetle [Cerambyx cerdo Linnaeus, 1758 (Coleoptera: Cerambycidae)]. Both of species are protected under the EU Habitats Directive and listed in the IUCN Red List of Threatened Species. The investigation of settling degree of C. cerdo was conducted at two areas, Ajameti and Vartsikhe, on the edge and in the middle of oak forests in June and October 2014. On both the areas, the number of trees with fly exit holes of great capricorn beetle was higher on the margin of forests where plantations suffer greater of human impact. The number of fly exit holes of imago is significantly increased due to deterioration of sanitary condition of forests. The measures of management conservation at Ajameti Managed Reserve were proposed