Precipitation Type Specific Radar Reflectivity-Rain Rate Relationships for Warsaw, Poland

Penelitian ini bertujuan untuk mengetahui peningkatan penguasaan konsep dan kemampuan literasi sains siswa dengan menggunakan model pembelajaran kontekstual berbantuan multimedia. Metode dan desain penelitian yang digunakan adalah quasi experiment dengan pretest-posttest control group design. Subjek penelitiannya adalah kelas XI di kabupaten Subang, Jawa-Barat. Hasil penelitian menunjukkan Model Pembelajaran Kontekstual berbantuan multimedia secara signifikan mampu meningkatkan penguasaan konsep dan kemampuan literasi sains siswa. Peningkatan penguasaan konsep siswa dengan nilai N-Gain 0.50 (kategori sedang) untuk kelas eksperimen dan 0,30 (kategori sedang) untuk kelas kontrol. Peningkatan kemampuan literasi sains siswa dengan nilai N-Gain 0.45 (kategori sedang) untuk kelas eksperimen dan 0,30 (kategori sedang) untuk kelas kontrol. This study aims to determine the concepts mastery and skills increase scientific literacy of students by using multimedia-assisted contextual learning model. The method used quasi experiment with pretest-posttest control group design. Subjects of study are class XI in Subang districts, West-Java. The result of study showed that contextual model’s aided by multimedia significantly enhance student’s concepts mastery and skills scientific literacy. The enhancement of student’s concepts mastery with N-Gain value is 0.50 (medium category) for experiment class and 0,30 (medium category) for control class. The enhancement of student's skills scientific literacy with N-Gain value is 0.45 (medium category) for experiment class and 0,30 (medium category) for control class

Population Carrier Rates of Pathogenic ARSA Gene Mutations: Is Metachromatic Leukodystrophy Underdiagnosed?

BACKGROUND: Metachromatic leukodystrophy (MLD) is a severe neurometabolic disease caused mainly by deficiency of arylsulfatase A encoded by the ARSA gene. Based on epidemiological surveys the incidence of MLD per 100,000 live births varied from 0.6 to 2.5. Our purpose was to estimate the birth prevalence of MLD in Poland by determining population frequency of the common pathogenic ARSA gene mutations and to compare this estimate with epidemiological data. METHODOLOGY: We studied two independently ascertained cohorts from the Polish background population (N∼3000 each) and determined carrier rates of common ARSA gene mutations: c.459+1G>A, p.P426L, p.I179S (cohort 1) and c.459+1G>A, p.I179S (cohort 2). PRINCIPAL FINDINGS: Taking into account ARSA gene mutation distribution among 60 Polish patients, the expected MLD birth prevalence in the general population (assuming no selection against homozygous fetuses) was estimated as 4.0/100,000 and 4.1/100,000, respectively for the 1(st) and the 2(nd) cohort with a pooled estimate of 4.1/100,000 (CI: 1.8-9.4) which was higher than the estimate of 0.38 per 100,000 live births based on diagnosed cases. The p.I179S mutation was relatively more prevalent among controls than patients (OR = 3.6, P = 0.0082, for a comparison of p.I179S frequency relative to c.459+1G>A between controls vs. patients). CONCLUSIONS/SIGNIFICANCE: The observed discrepancy between the measured incidence of metachromatic leukodystrophy and the predicted carriage rates suggests that MLD is substantially underdiagnosed in the Polish population. The underdiagnosis rate may be particularly high among patients with p.I179S mutation whose disease is characterized mainly by psychotic symptoms

Plant expression, lyophilisation and storage of HBV medium and large surface antigens for a prototype oral vaccine formulation

Current immunisation programmes against hepatitis B virus (HBV) increasingly often involve novel tri-component vaccines containing—together with the small (S-HBsAg)—also medium and large surface antigens of HBV (M- and L-HBsAg). Plants producing all HBsAg proteins can be a source of components for a potential oral ‘triple’ anti-HBV vaccine. The objective of the presented research was to study the potential of M/L-HBsAg expression in leaf tissue and conditions of its processing for a prototype oral vaccine. Tobacco and lettuce carrying M- or L-HBsAg genes and resistant to the herbicide glufosinate were engineered and integration of the transgenes was verified by PCR and Southern hybridizations. M- and L-HBsAg expression was confirmed by Western blot and assayed by ELISA at the level of micrograms per g of fresh weight. The antigens displayed a common S domain and characteristic domains preS2 and preS1 and were assembled into virus-like particles (VLPs). Leaf tissues containing M- and L-HBsAg were lyophilised to produce a starting material of an orally administered vaccine formula. The antigens were distinctly sensitive to freeze-drying conditions and storage temperature, in the aspect of stability of S and preS domains and formation of multimeric particles. Efficiency of lyophilisation and storage depended also on the initial antigen content in plant tissue, yet M-HBsAg appeared to be approximately 1.5–2 times more stable than L-HBsAg. The results of the study provide indications concerning the preparation of two other constituents, next to S-HBsAg, for a plant-derived prototype oral tri-component vaccine against hepatitis B