Struktur und Langzeitentwicklung von subalpinen Pinus montana Miller und Pinus cembra L. Wäldern in den zentraleuropäischen Alpen

Summary:: Since traditional agriculture and forestry are no longer economically viable in many regions of the European Alps, subalpine forests will become less managed or completely abandoned in the near future. Therefore, the interest in understanding how forest stands will develop after abandonment has increased considerably over the past two decades. While much is known about stand structure and stand development of Norway spruce (Picea abies L.) forests, almost no knowledge is available about the same processes in forest communities of the Central Alps. In the Swiss National Park (SNP), the forested area is comprised of mountain pine (Pinus montana Miller), Swiss stone pine/larch, (Pinus cembra L./Larix decidua L.). and mixed stands. When the Park was founded in 1914 all management activities were stopped. Therefore, this area offers the opportunity to study stand development and changes in stand structure after abandonment. We compared historic (1957) and present data (2001/02) from 19 stands that were grouped into characteristic stand types: "mountain pine”, "mixed”, and "stone pine”. We detected significant decreases in total tree density (stem/ha) and sapling density (saplings/ha) of 45 to 57%, and 64 to 76%, respectively, over the 45 years of observation for all stand types. These changes were strongly related to decreases in the number of shade intolerant mountain pine trees. Simultaneously, the amount of non-standing woody residue increased from less than 4 t/ha to 36 to67.7 t/ha, and the density of standing dead wood (stems/ha) decreased significantly between 72 and 94%. The biomass of standing dead wood (t/ha), however, changed only slightly between 1957 and 01/02. Our results describe the successional development of continental subalpine forests after abandonment and outlines changes that might take place in similar areas in the near futur

Characterization of Procoagulant COAT Platelets in Patients with Glanzmann Thrombasthenia.

Patients affected by the rare Glanzmann thrombasthenia (GT) suffer from defective or low levels of the platelet-associated glycoprotein (GP) IIb/IIIa, which acts as a fibrinogen receptor, and have therefore an impaired ability to aggregate platelets. Because the procoagulant activity is a dichotomous facet of platelet activation, diverging from the aggregation endpoint, we were interested in characterizing the ability to generate procoagulant platelets in GT patients. Therefore, we investigated, by flow cytometry analysis, platelet functions in three GT patients as well as their ability to generate procoagulant collagen-and-thrombin (COAT) platelets upon combined activation with convulxin-plus-thrombin. In addition, we further characterized intracellular ion fluxes during the procoagulant response, using specific probes to monitor by flow cytometry kinetics of cytosolic calcium, sodium, and potassium ion fluxes. GT patients generated higher percentages of procoagulant COAT platelets compared to healthy donors. Moreover, they were able to mobilize higher levels of cytosolic calcium following convulxin-plus-thrombin activation, which is congruent with the greater procoagulant activity. Further investigations will dissect the role of GPIIb/IIIa outside-in signalling possibly implicated in the regulation of platelet procoagulant activity

Structural Determinants of Health among Im/Migrants in the Indoor Sex Industry: Experiences of Workers and Managers/Owners in Metropolitan Vancouver

Background Globally, im/migrant women are overrepresented in the sex industry and experience disproportionate health inequities. Despite evidence that the health impacts of migration may vary according to the timing and stage of migration (e.g., early arrival vs. long-term migration), limited evidence exists regarding social and structural determinants of health across different stages of migration, especially among im/migrants engaged in sex work. Our aim was to describe and analyze the evolving social and structural determinants of health and safety across the arrival and settlement process for im/migrants in the indoor sex industry. Methods We analyzed qualitative interviews conducted with 44 im/migrant sex workers and managers/owners working in indoor sex establishments (e.g., massage parlours, micro-brothels) in Metropolitan Vancouver, Canada in 2011; quantitative data from AESHA, a larger community-based cohort, were used to describe socio-demographic and social and structural characteristics of im/migrant sex workers. Results Based on quantitative data among 198 im/migrant workers in AESHA, 78.3% were Chinese-born, the median duration in Canada was 6 years, and most (86.4%) serviced clients in formal indoor establishments. Qualitative narratives revealed diverse pathways into sex work upon arrival to Canada, including language barriers to conventional labour markets and the higher pay and relative flexibility of sex work. Once engaged in sex work, fear associated with police raids (e.g., immigration concerns, sex work disclosure) and language barriers to sexual negotiation and health, social and legal supports posed pervasive challenges to health, safety and human rights during long-term settlement in Canada. Conclusions Findings highlight the critical influences of criminalization, language barriers, and stigma and discrimination related to sex work and im/migrant status in shaping occupational health and safety for im/migrants engaged in sex work. Interventions and policy reforms that emphasize human rights and occupational health are needed to promote health and wellbeing across the arrival and settlement process

Characterization of Procoagulant COAT Platelets in Patients with Glanzmann Thrombasthenia

Patients affected by the rare Glanzmann thrombasthenia (GT) suffer from defective or low levels of the platelet-associated glycoprotein (GP) IIb/IIIa, which acts as a fibrinogen receptor, and have therefore an impaired ability to aggregate platelets. Because the procoagulant activity is a dichotomous facet of platelet activation, diverging from the aggregation endpoint, we were interested in characterizing the ability to generate procoagulant platelets in GT patients. Therefore, we investigated, by flow cytometry analysis, platelet function in three GT patients as well as their ability to generate procoagulant COAT platelets upon combined activation with convulxin-plus-thrombin. In addition, we further characterised intracellular ion fluxes during the procoagulant response using specific ion probes to monitor, by flow cytometry, the kinetics of cytosolic calcium, sodium, and potassium. GT patients generated higher percentages of procoagulant COAT platelets compared to healthy donors. Moreover, they were able to mobilize higher levels of cytosolic calcium following convulxin-plus-thrombin activation, which is congruent with the greater procoagulant activity. Further investigations will dissect the role of GPIIb/IIIa outside-in signalling possibly implicated in the regulation of platelet procoagulant activity

Work environments and HIV prevention: a qualitative review and meta-synthesis of sex worker narratives

Background: Sex workers (SWs) experience a disproportionately high burden of HIV, with evidence indicating that complex and dynamic factors within work environments play a critical role in mitigating or producing HIV risks in sex work. In light of sweeping policy efforts to further criminalize sex work globally, coupled with emerging calls for structural responses situated in labour and human-rights frameworks, this meta-synthesis of the qualitative and ethnographic literature sought to examine SWs’ narratives to elucidate the ways in which physical, social and policy features of diverse work environments influence SWs’ agency to engage in HIV prevention. Methods We conducted a meta-synthesis of qualitative and ethnographic studies published from 2008 to 2014 to elucidate SWs’ narratives and lived experiences of the complex and nuanced ways in which physical, social, and policy features of indoor and outdoor work environments shape HIV prevention in the sex industry. Results Twenty-four qualitative and/or ethnographic studies were included in this meta-synthesis. SWs’ narratives revealed the nuanced ways that physical, social, and policy features of work environments shaped HIV risk and interacted with macrostructural constraints (e.g., criminalization, stigma) and community determinants (e.g., sex worker empowerment initiatives) to shape SWs’ agency in negotiating condom use. SWs’ narratives revealed the ways in which the existence of occupational health and safety standards in indoor establishments, as well as protective practices of third parties (e.g., condom promotion) and other SWs/peers were critical ways of enhancing safety and sexual risk negotiation within indoor work environments. Additionally, working in settings where negative interactions with law enforcement were minimized (e.g., working in decriminalized contexts or environments in which peers/managers successfully deterred unjust policing practices) was critical for supporting SWs’ agency to negotiate HIV prevention. Conclusions Policy reforms to remove punitive approaches to sex work, ensure supportive workplace standards and policies, and foster SWs’ ability to work collectively are recommended to foster the realization of SWs’ health and human rights across diverse settings. Future qualitative and mixed-methods research is recommended to ensure that HIV policies and programmes are grounded in SWs’ voices and realities, particularly in more under-represented regions such as Eastern Europe and Sub-Saharan Africa.Medicine, Faculty ofOther UBCNon UBCMedicine, Department ofReviewedFacult

Oral vitamin D - Is it necessary to be taken with meals containing fat?

Vitamin D is fat-soluble. It is suggested to be taken with meals containing fat to enhance its intestinal absorption. However, there are only a few controlled studies which investigate prandial inferences on its absorption. Within a group of 13 general practitioners, during a period of three months, we ran two identical study protocols during winter and early springtime. Blood was taken from the subjects, 60’000 units of vitamin D (cholecalciferol) in 3 ml of oil was ingested, and blood was sampled once again a week later. In a randomized cross-over design, one time the vitamin was ingested postprandially, and another time in a fasting state. The basal levels of 25-hydroxyvitamin D were 45 (28) (median, interquartile range) before fasting, and 40 (15) nmol/l before postprandial dosing. One week thereafter, serum 25-hydroxyvitamin D was increased by 13 (21), when the supplement was ingested in a fasting state, while taken after a meal containing fat, the rise was 25 (17) nmol/l (n.s., p=0.13). The other parameters which were tested remained unchanged throughout the study: calcium, phosphate, albumin, and 1,25?dihydroxyvitamin D (calcitriol). Our preliminary data do not support major influences on vitamin D absorption by the influence of fasting vs. concomitant fatty meal intake. Vitamin D oral loading doses can be applied irrespective of prandial state, while its maintenance dosing by the patients should still take place after a meal containing fat. Absorption of vitamin D seems to be unpredictable – fractionate dosage and determination of 25-hydroxyvitamin D during treatment may be helpful. Unexplained low serum levels after repeated supplementation could be due to intestinal disease, mainly celiac

An Evaluation of Indoor Sex Workers’ Sexual Health Access in Metro Vancouver: Applying an Occupational Health & Safety Lens in the Context of Criminalization

The criminalization of sex work has been consistently shown to undermine workers’ Occupational Health and Safety (OHS), including sexual health. Drawing on the ‘Guide to OHS in the New Zealand Sex Industry’ (the Guide), we assessed barriers to sexual health best practices among indoor sex workers in Metro Vancouver, Canada, in the context of ongoing criminalization. Part of a longstanding community-based study, this analysis drew on 47 qualitative interviews (2017–2018) with indoor sex workers and third parties. Participants’ narratives were analyzed drawing on a social determinants of health framework and on the Guide with specific focus on sexual health. Our findings suggest that sex workers and third parties utilize many sexual health strategies, including use of Personal Protective Equipment (PPE) and peer-driven sexual health education. However, participant narratives demonstrate how structural factors such as criminalization, immigration, and stigma limit the accessibility of additional OHS best practices outlined in the Guide and beyond, including access to non-stigmatizing sexual health assessments, and distribution of diverse PPE by third parties. Our current study supports the need for full decriminalization of sex work, including im/migrant sex work, to allow for the uptake of OHS guidelines that support the wellbeing and autonomy of all sex workers