A rare case of hard palate radicular cyst

Inflammatory odontogenic cysts comprise the entity of radicular cysts which may affect bone jaws to a different extend. Most of these lesions encompass the highest structure – the dental apex and are well defined and delineated from adjacent benign structures. Concerning different clinical characteristics of the remaining odontogenic cysts differential diagnosis includes: residual cyst, follicular cyst, keratocyst, non-odontogenic fissural cysts as well as some malignant diseases including ameloblastoma, cementoma, Pindborg tumor, etc. This article presents a sole case of a massive odontogenic radicular cyst, affecting the right half of the hard palate at the area of lacking molars, crossing midline and containing dental apex. The case was diagnosed as infected radicular cyst on the basis of clinical, histopathological and radiological findings. The clinical characteristics of this cyst represent an interesting and extraordinary finding due to the presence of a foreign body in it, situated far from the upper left side teeth, extracted several years before its appearance. The lesion was surgically enucleated including the apex when all adjacent teeth and vital oral structures were kept intact. No postoperative complications were endured by the patient and normal healing process wasachieved

Extramammary Paget’s Disease Versus Lichen Sclerosus

Burning, itching and dyspareunia are typical symptoms of many genital diseases. These subjective complaints can be misdiagnosed because of different clinical presentations. We present a case report of a postmenopausal woman treated for genital warts over a period of three years followed by the development of leukoplastic lesions in the whitish area clinical classified as Lichen sclerosus (LS). Histology of this lesion revealed carcinoma in situ. After radical surgical removal, vulvar Paget’s disease was histologically verified. LS and extramammary Paget's disease (EMPD) belong to a group of uncommon dermatoses which mainly affect the skin of the genitals in postmenopausal women. Ulceration, erosions and leukoplastic lesions can signalize the development of squamous cell carcinoma in association of lichen sclerosus, on the other hand, they can be the sign of EMPD after a long period of time using different topical agents. The importance of reaching the correct diagnosis is essential and can influence current patient investigations and invasive or non-invasive treatment

Practical use of High-Resolution 20 MHz Ultrasonography in Dermatovenerology

High-frequency skin ultrasonography using Dermascan C, manufactured by Cortex Technology, is an important part of modern diagnostic procedure of various skin diseases. It has been used in dermatology since 1979, when it was first used for the measurement of cutaneous thickness. Ultrasonography is a universal, painless, low-risk and non-invasive procedure that can easily be performed and repeated. It provides real-time visual information about the processes in the skin. This technique has grown to become frequent imaging method in dermatology

Late mitotic failure in mice lacking Sak, a polo-like kinase

AbstractPolo-like kinases in yeast, flies, and mammals regulate key events in mitosis. Such events include spindle formation at G2/M, the anaphase-promoting complex (APC) at the exit from mitosis, the cleavage structure at cytokinesis, and DNA damage checkpoints in G2/M. Polo-like kinases are distinguished by two C-terminal polo box (pb) motifs, which localize the enzymes to mitotic structures. We previously identified Sak, a novel polo-like kinase found in Drosophila and mammals. Here, we demonstrate that the Sak kinase has a functional pb domain that localizes the enzyme to the nucleolus during G2, to the centrosomes in G2/M, and to the cleavage furrow during cytokinesis. To study the role of Sak in embryo development, we generated a Sak null allele, the first polo-like kinase to be mutated in mice. Sak−/− embryos arrested after gastrulation at E7.5, with a marked increase in mitotic and apoptotic cells. Sak−/− embryos displayed cells in late anaphase or telophase that continued to express cyclin B1 and phosphorylated histone H3. Our results suggest that Sak is required for the APC-dependent destruction of cyclin B1 and for exit from mitosis in the postgastrulation embryo

Cell adhesion and growth on ultrananocrystalline diamond and diamond-like carbon films after different surface modifications

Diamond and diamond-like carbon (DLC) films possess a set of excellent physical and chemical properties which together with a high biocompatibility make them attractive candidates for a number of medical and biotechnological applications. In the current work thin ultrananocrystalline diamond (UNCD) and DLC films were comparatively investigated with respect to cell attachment and proliferation after different surface modifications. The UNCD films were prepared by microwave plasma enhanced chemical vapor deposition, the DLC films by pulsed laser deposition (PLD). The films were comprehensively characterized with respect to their basic properties, e.g. crystallinity, morphology, chemical bonding nature, etc. Afterwards the UNCD and DLC films were modified applying O2 or NH3/N2 plasmas and UV/O3 treatments to alter their surface termination. The surface composition of as-grown and modified samples was studied by X-ray photoelectron spectroscopy (XPS). Furthermore the films were characterized by contact angle measurements with water, formamide, 1-decanol and diiodomethane; from the results obtained the surface energy with its dispersive and polar components was calculated. The adhesion and proliferation of MG63 osteosarcoma cells on the different UNCD and DLC samples were assessed by measurement of the cell attachment efficiency and MTT assays. The determined cell densities were compared and correlated with the surface properties of as-deposited and modified UNCD and DLC films.JRC.I.4-Nanobioscience