29 research outputs found
The Mechanism of Peptide Hydrolysis Catalysed by Dipeptidyl Peptidase III from Bacteroides thetaiotaomicron
Dipeptidyl peptidase III (DPP III) is a zinc- dependent peptidase that cleaves dipeptides off of N-termini of its substrates. Previous studies on human DPP III reveal a reaction mechanism similar to that of thermolysin. Since the active site is conserved within the DPP III family, it is not surprising that the mechanism determined for Bacteroides thetaiotaomicron DPP III (BtDPP III) closely resembles that of hDPP III. However, the hydrogen bond network within the model differs slightly from that in the human ortholog, which results in two proposed pathways. The calculated Gibbs activation energy of 90.1 kJ molā1 is larger than the one calculated from kinetic data for the preferred substrate Arg2-2-naphthylamide at room temperature (69 kJ molā1), suggesting the importance of treating the whole DPP III enzyme in the calculations
Towards the Strongest Neutral Organic Superbases Based on Intramolecular H-bonds
Utilizing several different trialkylarsine oxides and substituted pyridine N-oxides as a hydrogen bond acceptors in tri-substituted guanidines we designed several very basic superbases possessing intramo-lecular hydrogen bonds (IHB-superbases), with proton affinity in the gas phase that comes very close to that of paradigmatic P4-tBu Schwesinger superbase and with pKa in acetonitrile up to 36
units
Utjecaj vinskih sorta na aromu destilata
Aroma is a phenomenon that occurrs when food and live organism mutually react during the process of consumption. Many factors influence the making of aroma of wine distillates (e.g. cognac) during the technological process of distillation. It is extremely difficult to bring an objective judgement on the influence of individual factors since aroma is very subjective in its nature. The possibility of objective assessment of the influence of grape varieties on wine distillate was performed in this work using a computer in processing the results of cromatographic analyses of aroma substances. The given results were verified by sensor analyses. The achieved results have shown that mathematical model for calculating aroma chromatogram similarity can be successfully used for objective assessment of the influence of individual factors on aroma of wine distillates and that grape varieties have significant impact on aroma and the quality of wine distillates.Aroma je fenomen koji se pojavljuje tijekom konzumiranja hrane u organizmu. Pri stvaranju ukupne kakvoÄe (tip cognac) vinskih destilata upravo taj fenomen ima dominirajuÄu, a vrlo Äesto i odluÄujuÄu ulogu. Na aromu vinskih destilata utjeÄe mnogo Äinitelja tijekom njezina stvaranja u tehnoloÅ”kom procesu proizvodnje. MeÄutim, objektivnu je prosudbu o utjecaju pojedinog Äinitelja iznimno teÅ”ko donijeti jer aroma ima i subjektivni karakter. U ovom je radu ispitana moguÄnost objektivne procjene utjecaja sorte na aromu vinskih destilata primjenom raÄunala u obradbi kromatografskih rezultata analize tvari arome, a navedeni su rezultati provjereni senzorskim analizama. Dobiveni su rezultati pokazali da se matematiÄki model za kompjutorsko izraÄunavanje sliÄnosti kromatograma arome može uspjeÅ”no koristiti pri objektivnoj procjeni utjecaja pojedinih Äinitelja na aromu vinskih destilata, te da sorta grožÄa bitno utjeÄe na aromu i kakvoÄu vinskih destilata
RAZVOJ EKSPLOATACIJE MINERALNIH SIROVINA ZA PROIZVODNJU CEMENTA NA POVRÅ INSKIM KOPOVIMA Ā»PARTIZANĀ« I Ā»PRVOBORACĀ«
The article presents the exploitation of mineral raw materials for the cement industry of Split from the beginning of this century till today. Geological and geomcchanical characteristic of the open pits Ā»PartizanĀ« and "PrvoboracĀ«, located within the same deposit and the technological exploitation process with basic parameters of suecessibility in both pits arc detaily discussed. The works of open pits\u27 reclamation which has to be carried out simultanouslly with the exploitation itself arc also considered, as well as the perspectives of further exploitation development, in accord with modern tendencies to the mining technology developmentU Älanku je iznesen prikaz eksploatacije mineralnih sirovina za cementnu industriju Splita, od poÄetka ovog stoljeÄa do danas, Dataljnije su obraÄene geoloÅ”ke i geomehaniÄke karakteristike kopova Ā»PartizanĀ« i Ā»PrvoboracĀ« koji se nalaze unutar istog ležiÅ”ta, te tehnoloÅ”ki proces eksploatacije s osnovnim pokazateljima uspjeÅ”nosti na oba kopa. TakoÄer, prikazani su radovi na sanaciji kopova, koju je potrebno provoditi paralelno sa samom eksploatacijom. KonaÄno, prikazane su perspektive daljnjeg razvoja eksploatacije u skladu sa suvremenim tendencijama razvoja rudarske tehnologij
KraÄe motornih vozila kao savremeni vid organizovanog kriminala u domaÄoj kriminalistiÄkoj praksi
The first part of the paper outlines the normative and legal framework within which criminal law provides protection of motor vehicles and lists the problems encountered by the judiciary in the practical implementation of legal provisions. The central part of the paper focuses on the modus operandi of organized criminal groups engaged in the perpetration of such criminal acts, particularly in the organization and deployment of various electronic tools used to overcome electronic protection of modern vehicle makes. Finally, the authors emphasize the fact that investigative activities aimed at tracking down organized criminal groups and identifying the roles of their members call for an approach that differs from that used for detecting other criminal acts and point out to the main guidelines for police activities aimed at dealing with such cases.U prvom delu rada autori ukazuju na normativno-pravni okvir kojim se obezbeÄuje kriviÄno-pravna zaÅ”tita motornih vozila i na probleme koji se pojavljuju u njegovoj praktiÄnoj primeni od strane policije i pravosuÄa. Centralni deo rada tretira modus operandi organizovanih kriminalnih grupa vezan za ovu vrstu kriviÄnih dela, a u prvom redu organizaciju kriminalne aktivnosti i primenu razliÄitih elektronskih alata u cilju savlaÄivanja elektronske zaÅ”tite prisutne na novijim tipovima vozila. Na kraju, autori ukazuju na Äinjenicu da kriminalistiÄka delatnost razotkrivanja organizovanih kriminalnih grupa oformljenih za vrÅ”enje kriviÄnih dela kraÄa vozila, njihove strukture i uloge pojedinih Älanova, zahteva drugaÄiji pristup u odnosu na druga kriviÄna dela, te ukazuju na osnovne pravce policijske delatnosti koja se preduzima u tom cilju
Održiva poljoprivreda - znaÄaj adaptacije agrotehniÄkih mera u proizvodnji ozime pÅ”enice
The objectives of this study were to examine influence conventional vs low-input technology on some important soil physical properties, weed control and grain yield of winter wheat. The main conclusion that can be drawn from this result is that the significantly bulk density has been reached in NT system. The implication of the results first figure can be seen in two next soil physical properties total porosity and air-filled porosity were significantly lower compared to CT and MT system, as a consequence of higher bulk density. The significantly higher the total number of weeds have been recorded, under NT what is expected tendency and it was especially to perennial weeds. Conventional tillage had better effect in weed control than both of conservation tillage systems had. Through this results we can see that low input cultivars yielded better than high input cultivars. The low-input cultivars out yielded than high-input cultivars. Alternative small grains (Tr. aestivum ssp compactum, Tr, durum and Tr. Spelta) gives lower grain yield compared with commercial cultivar NS-40S.Održiva poljoprivreda je kompleks pronalaženja novih puteva praÄen brojnim rizicima. Tu su rizici koji mogu biti praÄeni smanjenjem prinosa i brojnim nepoznanicama u zaÅ”titi bilja od bolesti i Å”tetoÄina, ali i nekim drugim. Prelazak sa konvencionalnih sistema gajenja na racionalne zahteva brojne promene u tehnologiji gajenja. Tehnologije nižih ulaganja u proizvodnji ozime pÅ”enice, uopÅ”teno gledajuÄi, zahtevaju adaptaciju najvažnijih mera i sastoje se od: redukovanih sistema obrade zemljiÅ”ta, nižih normi Äubrenja mineralnim Äubrivima, upotrebe mikrobioloÅ”kih Äubriva, veÄe diversifikacije useva u plodoredu, itd. Racionalne tehnologije gajenja u proizvodnji ozime pÅ”enice mogu znaÄajno doprineti smanjenju zemljiÅ”ne erozije, znaÄajnom smanjenju troÅ”kova, odnosno veÄoj ekonomskoj efikasnosti, i to bez veÄeg smanjenja prinosa. Redukcije u primeni agrotehniÄkih mera mogu biti izvor stresa (mehaniÄki, suÅ”a). Zato ovu Äinjenicu moraju imati u vidu oplemenjivaÄi bilja koji Äe stvarati sorte koje bi u novostvorenim uslovima reagovale veÄom efikasnoÅ”Äu. Nove tehnologije podrazumevaju veÄu fleksibilnost u primeni pojedinih agrotehniÄkih mera (obrade zemljiÅ”ta, Äubrenja, zaÅ”tite od bolesti, Å”tetoÄina i korova, plodoreda) sa pažljivo odabranim sortama za te uslove. Cilj ovog rada je da ukaže na znaÄaj kreiranja održivih tehnologija gajenja ozime pÅ”enice i njihov uticaj na fiziÄke osobine zemljiÅ”ta, kontrolu korova i prinos