NLRC5 Functions beyond MHC I Regulation—What Do We Know So Far?

NLRC5 is a member of the NLR family that acts as a transcriptional activator of MHC class I genes. In line with the function of several related NLR proteins in innate immune responses, there is, however, also ample evidence that NLRC5 contributes to innate and adaptive immune responses beyond the regulation of MHC class I genes. In human and murine cells, for example, NLRC5 was proposed to contribute to inflammatory and type I interferon responses. The role of NLRC5 in these and other cellular processes is hitherto still not well understood and blurred by discrepancies in the reported data. Here, we provide a detailed and critical discussion of the available experimental data on the emerging biological functions of NLRC5 in innate immune responses in men and mice. Better awareness of the multiple roles of NLRC5 will help to define its overall contribution to immune responses and cancer

A humán NOD-like receptor NLRC5 fehérje vizsgálata

A Flag-fúziós fehérjével jelölt NLRC5 fehérjét 293T sejtekben expresszáltattunk. A fúziós fehérje kimutatása során Western-blot eljárásban anti-flag és anti-NLRC5 antitesteket használtunk fel és teszteltünk. Az anti-flag antitestek használatával sikeresen kimutattuk az overexpresszált fehérjét, ezzel szemben az anti-NLRC5 antitestek kevésbé bizonyultak hatékonynak a flag-tagged NLRC5 fehérje kimutatásában. Az expresszáltatott NLRC5 fehérje jelenlétét Western-blot eljárással sikerült kimutatni, az eljárás során anti-flag antitesteket használtunk. Az NLRC5 molekula tömegének meghatározása érdekében teljes sejtlizátumból végeztünk Gélszűrést, az eredmények alapján feltételezhető az overexpresszált NLRC5 oligomerizációja.MSc/MABiotechnológiag

The NLRP3 inflammasome - interleukin 1 pathway as a therapeutic target in gout

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Caffeine Has Different Immunomodulatory Effect on the Cytokine Expression and NLRP3 Inflammasome Function in Various Human Macrophage Subpopulations

Besides its well-known psychoactive effects, caffeine has a broad range of actions. It regulates several physiological mechanisms as well as modulates both native and adaptive immune responses by various ways. Although caffeine is assumed to be a negative regulator of inflammation, the effect on the secretion of pro- and anti-inflammatory cytokines is highly controversial. Macrophages are major mediators of inflammatory responses; however, the various subpopulations develop different effects ranging from the initiation to the resolution of inflammation. Here we report a comparative analysis of the effect of caffeine on two subpopulations of human monocyte-derived macrophages differentiated in the presence of macrophage colony-stimulating factor (M-CSF) or granulocyte-macrophage colony-stimulating factor (GM-CSF), resulting in M-MΦs and GM-MΦs, respectively. We showed that although TNF-α secretion was downregulated in both LPS-activated MΦ subtypes by caffeine, the secretion of IL-8, IL-6, and IL-1β as well as the expression of Nod-like receptors was enhanced in M-MΦs, while it did not change in GM-MΦs. We showed that caffeine (1) altered adenosine receptor expression, (2) changed Akt/AMPK/mTOR signaling pathways, and (3) inhibited STAT1/IL-10 signaling axis in M-MΦs. We hypothesized that these alterations play an important modulatory role in the upregulation of NLRP3 inflammasome-mediated IL-1β secretion in LPS-activated M-MΦs following caffeine treatment

Immunogenic Dendritic Cell Generation from Pluripotent Stem Cells by Ectopic Expression of Runx3

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