136 research outputs found

    Magnetic levitation of large liquid volume

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    It is well known from experiments and industrial applications of cold crucible melting that an intense AC magnetic field can be used to levitate large volumes of liquid metal in the terrestrial conditions. The levitation confinement mechanism for large volumes of fluid is considerably different from the case of a small droplet, where surface tension plays a key role in constraining the liquid outflow at the critical bottom point. The dynamic interaction between the oscillatory motion of the free surface and the effects of turbulent flow is analysed using a unified numerical model, which describes the time dependent behaviour of the liquid metal and the magnetic field. The MHD modified k-? turbulence model is used to describe the mixing and damping properties at smaller scales not resolved by the macro model. The numerical multiphysics simulations suggest that it is possible to levitate a few kilograms of liquid metal in a cold crucible without requiring mechanical support from the container walls. Possible applications to the processing of reactive metals are discussed

    Use of a Static Magnetic Field in Measuring the Thermal Conductivity of a Levitated Molten Droplet

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    Numerical models are used to analyze the complex behaviour of magnetically levitated droplets in the context of determining their thermophysical properties. We focus on a novel method reported in Tsukada et al. [4] which uses periodic laser heating to determine the thermal conductivity of an electromagnetically levitated droplet in the presence of a static DC field to suppress convection. The results obtained from the spectral-collocation based free surface code SPHINX and the commercial package COMSOL independently confirm and extend previous findings in [4]. By including the effects of turbulence and movement of the free surface SPHINX can predict the behaviour of the droplet in dynamic regimes with and without the DC magnetic field. COMSOL is used to investigate arbitrary amplitude axial translational oscillations when the spherical droplet is displaced off its equilibrium. The results demonstrate that relatively small amplitude oscillations could cause significant variation in Joule heating and redistribution of the temperature. The effect of translational oscillations on the lumped circuit inductance is analysed. When a fixed voltage drive is applied across the terminals of the levitation coil, this effect will cause the coil current to change and a correction is needed to the electromagnetic force acting on the droplet

    Contactless ultrasound generation in a crucible

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    Ultrasound treatment is used in light alloys during solidification to refine microstructure, remove gas, or disperse immersed particles. A mechanical sonotrode immersed in the melt is most effective when probe tip vibrations lead to cavitation. Liquid contact with the probe can be problematic for high temperature or reactive melts leading to contamination. An alternative contactless method of generating ultrasonic waves is proposed, using electromagnetic (EM) induction. As a bonus, the EM force induces vigorous stirring distributing the effect to treat larger volumes of material. In a typical application, the induction coil surrounding the crucible— also used to melt the alloy—may be adopted for this purpose with suitable tuning. Alternatively, a top coil, immersed in the melt (but still contactless due to EM force repulsion) may be used. Numerical simulations of sound, flow, and EM fields suggest that large pressure amplitudes leading to cavitation may be achievable with this method

    Architecturally diverse proteins converge on an analogous mechanism to inactivate Uracil-DNA glycosylase

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    Uracil-DNA glycosylase (UDG) compromises the replication strategies of diverse viruses from unrelated lineages. Virally encoded proteins therefore exist to limit, inhibit or target UDG activity for proteolysis. Viral proteins targeting UDG, such as the bacteriophage proteins ugi, and p56, and the HIV-1 protein Vpr, share no sequence similarity, and are not structurally homologous. Such diversity has hindered identification of known or expected UDG-inhibitory activities in other genomes. The structural basis for UDG inhibition by ugi is well characterized; yet, paradoxically, the structure of the unbound p56 protein is enigmatically unrevealing of its mechanism. To resolve this conundrum, we determined the structure of a p56 dimer bound to UDG. A helix from one of the subunits of p56 occupies the UDG DNA-binding cleft, whereas the dimer interface forms a hydrophobic box to trap a mechanistically important UDG residue. Surprisingly, these p56 inhibitory elements are unexpectedly analogous to features used by ugi despite profound architectural disparity. Contacts from B-DNA to UDG are mimicked by residues of the p56 helix, echoing the role of ugi’s inhibitory beta strand. Using mutagenesis, we propose that DNA mimicry by p56 is a targeting and specificity mechanism supporting tight inhibition via hydrophobic sequestration
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