Additional file 1 of Prospects for the development of community-based care in remote rural areas: a stakeholder analysis in Laos

Additional file 1: Interview guides. Translation of the qualitative interview guides

Comparisons of the prevalence of <i>T</i>. <i>whipplei</i> carriage in children feces between Senegal and Laos.

<p>Comparisons of the prevalence of <i>T</i>. <i>whipplei</i> carriage in children feces between Senegal and Laos.</p

Genetic diversity of 42 genotypes of <i>Tropheryma whipplei</i> obtained from 277 samples.

<p>Phylogenetic trees were constructed using the maximum likelihood method based on the Tamura 3-parameter substitution model. The countries in which the genotype has been detected are presented opposite the genotype, and the number of different individuals in which the genotype has been detected is provided in parentheses. The genotypes of each highly variable genomic sequence (HVGS) have intervened in 22, 13, 17 and 7 different combinations, respectively.</p

Reports of apparent mixed infections in Asia that included rickettsioses.

<p>Reports of apparent mixed infections in Asia that included rickettsioses.</p

Overview of PCR-based and DNA sequencing results.

*<p>positivity criteria in analogy to the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0002163#pntd.0002163-Bustin1" target="_blank">[19]</a>. GenBank accession numbers:</p>1<p>BankIt1587796 Seq2 KC283067.</p>2<p>BankIt1587796 Seq3 KC283068.</p>3<p>BankIt1587796 Seq1 KC283066.</p

Results of RDT stored at 35°C and in the hut in comparison to RDT stored at 4°C.

<p>Results of RDT stored at 35°C and in the hut in comparison to RDT stored at 4°C.</p

Comparison of agreements of the different techniques for RNA preparation with the neat serum extraction for Salavan Provincial Hospital sample.

<p>Comparison of agreements of the different techniques for RNA preparation with the neat serum extraction for Salavan Provincial Hospital sample.</p

Number of patients positive by DENV.

<p>All RT-PCR performed after extraction from RDT or filter paper and from neat serum for 362 patients admitted at Salavan Provincial Hospital.</p

Mean number of dengue RNA copies/μl recovered by extraction from RDT S, WS parts and filter paper, compared to direct extraction.

<p>Mean number of dengue RNA copies/μl recovered by extraction from RDT S, WS parts and filter paper, compared to direct extraction.</p

SYBR Green Real-Time PCR for the Detection of All Enterovirus-A71 Genogroups

<div><p>Enterovirus A71 (EV-A71) has recently become an important public health threat, especially in South-East Asia, where it has caused massive outbreaks of Hand, Foot and Mouth disease every year, resulting in significant mortality. Rapid detection of EV-A71 early in outbreaks would facilitate implementation of prevention and control measures to limit spread. Real-time RT-PCR is the technique of choice for the rapid diagnosis of EV-A71 infection and several systems have been developed to detect circulating strains. Although eight genogroups have been described globally, none of these PCR techniques detect all eight. We describe, for the first time, a SYBR Green real-time RT-PCR system validated to detect all 8 EV-A71 genogroups. This tool could permit the early detection and shift in genogroup circulation and the standardization of HFMD virological diagnosis, facilitating networking of laboratories working on EV-A71 in different regions.</p></div