Functional classification of protein toxins as a basis for bioinformatic screening

Proteins are fundamental to life and exhibit a wide diversity of activities, some of which are toxic. Therefore, assessing whether a specific protein is safe for consumption in foods and feeds is critical. Simple BLAST searches may reveal homology to a known toxin, when in fact the protein may pose no real danger. Another challenge to answer this question is the lack of curated databases with a representative set of experimentally validated toxins. Here we have systematically analyzed over 10,000 manually curated toxin sequences using sequence clustering, network analysis, and protein domain classification. We also developed a functional sequence signature method to distinguish toxic from non-toxic proteins. The current database, combined with motif analysis, can be used by researchers and regulators in a hazard screening capacity to assess the potential of a protein to be toxic at early stages of development. Identifying key signatures of toxicity can also aid in redesigning proteins, so as to maintain their desirable functions while reducing the risk of potential health hazards

Advancing Community Resilience through Community Radio: The Case of EK-FM in Western Kenya

Community radio can serve as a crucial channel to disseminate information in rural locations. This is the case of the Ekialo Kiona Community Youth Radio (EK-FM) on Mfangano Island, Kenya. EK-FM has been a platform for communicating health information during the outbreak of COVID-19. Now, over one year into the pandemic, our MDP student team alongside EK-FM staff are assessing community needs and EK-FM’s opportunities and limitations in meeting them. Motivated by the current negative impacts of COVID-19 on less privileged communities, we are developing organizational, financial, social, and strategic routes to enhance the radio’s ability to uphold its community resilience goals. By engaging Mfangano’s communities to understand the context surrounding EK-FM, we facilitate the discovery of community aspirations for the radio to connect Mfangano residents regardless of their location, whether about local Suba culture, health, or ecological issues. We also explore possible social entrepreneurial pathways and partnerships toward EK-FM’s lasting financial viability

Editor's Choice:Evaluating the potential for adverse interactions within genetically engineered breeding stacks

When genetically engineered stacks (also known as stacked or combined events) are produced by combining two or more single transgenic events by conventional breeding, the potential for interactions between the products of the events that impact food and feed safety can be evaluated and the need for further safety assessment can be determined on a case-by-case basis

Assessment of potential adjuvanticity of Cry proteins

Genetically modified (GM) crops have achieved success in the marketplace and their benefits extend beyond the overall increase in harvest yields to include lowered use of insecticides and decreased carbon dioxide emissions. The most widely grown GM crops contain gene/s for targeted insect protection, herbicide tolerance, or both. Plant expression of Bacillus thuringiensis (Bt) crystal (Cry) insecticidal proteins have been the primary way to impart insect resistance in GM crops. Although deemed safe by regulatory agencies globally, previous studies have been the basis for discussions around the potential immuno-adjuvant effects of Cry proteins. These studies had limitations in study design. The studies used animal models with extremely high doses of Cry proteins, which when given using the ig route were co-administered with an adjuvant. Although the presumption exists that Cry proteins may have immunostimulatory activity and therefore an adjuvanticity risk, the evidence shows that Cry proteins are expressed at very low levels in GM crops and are unlikely to function as adjuvants. This conclusion is based on critical review of the published literature on the effects of immunomodulation by Cry proteins, the history of safe use of Cry proteins in foods, safety of the Bt donor organisms, and pre-market weight-of-evidence-based safety assessments for GM crop

The COMPARE Database:A Public Resource for Allergen Identification, Adapted for Continuous Improvement

Motivation: The availability of databases identifying allergenic proteins via a transparent and consensus-based scientific approach is of prime importance to support the safety review of genetically-modified foods and feeds, and public safety in general. Over recent years, screening for potential new allergens sequences has become more complex due to the exponential increase of genomic sequence information. To address these challenges, an international collaborative scientific group coordinated by the Health and Environmental Sciences Institute (HESI), was tasked to develop a contemporary, adaptable, high-throughput process to build the COMprehensive Protein Allergen REsource (COMPARE) database, a publicly accessible allergen sequence data resource along with bioinformatics analytical tools following guidelines of FAO/WHO and CODEX Alimentarius Commission.Results: The COMPARE process is novel in that it involves the identification of candidate sequences via automated keyword-based sorting algorithm and manual curation of the annotated sequence entries retrieved from public protein sequence databases on a yearly basis; its process is meant for continuous improvement, with updates being transparently documented with each version; as a complementary approach, a yearly key-word based search of literature databases is added to identify new allergen sequences that were not (yet) submitted to protein databases; in addition, comments from the independent peer-review panel are posted on the website to increase transparency of decision making; finally, sequence comparison capabilities associated with the COMPARE database was developed to evaluate the potential allergenicity of proteins, based on internationally recognized guidelines, FAO/WHO and CODEX Alimentarius Commissio