Properties of magnetron hydroxyapatite coatings deposited on oxidized substrates

Hydroxyapatite (HA) coating were formed on oxidized niobium surfaces by the highfrequency magnetron sputtering method using hydroxyapatite and tricalcium phosphate targets. The structure, substructure and mechanical properties of the Nb–Nb2O5–HA system were investigated by X-ray diffraction, atomic force microscopy and nanoindentation and the stress state was assessed. The synthesized hydroxyapatite film had the following characteristics: thermal expansion coefficient 10–5 K–1; modulus of elasticity 120 GPa; adhesive strength not less than 0.45 kg/mm2; density 2900 kg/m3. The stress magnitude in the metal oxide substrate was from 11 to 14 MPa after hydroxyapatite film deposition

SUSY transformations with complex factorization constants. Application to spectral singularities

Supersymmetric (SUSY) transformation operators corresponding to complex factorization constants are analyzed as operators acting in the Hilbert space of functions square integrable on the positive semiaxis. Obtained results are applied to Hamiltonians possessing spectral singularities which are non-Hermitian SUSY partners of selfadjoint operators. A new regularization procedure for the resolution of the identity operator in terms of continuous biorthonormal set of the non-Hermitian Hamiltonian eigenfunctions is proposed. It is also shown that the continuous spectrum eigenfunction has zero binorm (in the sense of distributions) at the singular point.Comment: Thanks to A. Sokolov a number of inaccuracies are correcte

Perspectives approach to the assessment of the quality of the vaccine plague live in terms of immunogenicity. Epidemiology and Vaccinal Prevention

Research objective – studying of a possibility of application antigen – stimulated cellular in vitro tests and technology of the cytometric analysis for control of immunogene activity of batches of vaccine plague live.Materials and methods. As biomodels used white laboratory mice, immunized commercial medicine of vaccine of the plague NIIEG line, live from a strain of Yersinia pestis EV, in doses – 8 х 102, 4 х 103, 2 х 104 and 1 х 105 of living microbic cells. Blood for a research was taken from intact mice and on 7, 14 and 21 days after immunization. The intensity of an antigenreaktivnost of lymphocytes was defined in cellular in vitro tests, analyzing a marker of early activation (CD45+CD3+CD25+) of lymphocytes with use of the monoclonal antibodies conjugated from fluorokhroma. As specific antigen used a complex of water-soluble antigens of a plague microbe.Results. As a result of a research it is shown that at the animals vaccinated by doses 4 х 103 – 1 х 105 living microbic cells, the highest level of an expression activation marker lymphocytes at anti-gene stimulation of in vitro is registered on 14 days after immunization, at the same time the quantity of CD25 – positive lymphocytes are on average 6.8 times higher, than in control group. High degree of direct link (coefficient of correlation of r = 1,000) quantities of the survived animals with increase in level of lymphocytes, expressiruyushchy markers of early activation – CD25 is established.Conclusions. The offered technique can be used as the additional test when studying degree of immunogenicity of new (kandidatny) vaccines against plague

OPTIMIZATION OF THE METHOD OF OBTAINING PESTINE PP AND STUDYING ITS SPECIFIC ACTIVITY IN VITRO

The formation of immunity against infectious diseases is accompanied by an immunoallergic alteration of the organism, while the intensity of the allergic reaction is associated with the presence of specific immunity. Skin allergotesting is often used to determine the intensity of sensitization of the body. When determining the immunity of vaccines against the plague, previously an allergen was suggested as a pestin PP — a polypeptide polysaccharide complex of a plague microbe. The authors optimized the technique for obtaining the preparation of pestin with preservation of its chemical composition, high specificity and allergenic activity. It is known that a lack of allergic test in vivo is a high risk of formation of adverse reactions. A method for estimating adaptive antiplague immunity with the allergen pestin in antigen-specific cellular tests in vitro is proposed. The preparation of the allergen by a modified procedure was carried out by hydrolysis of the biomass of the vaccine strain of the plague microbe Yersinia pestis EV of the NIIEG line, followed by filtration and lyophilization of the precipitate. In the preparation obtained, the pH and the protein concentration were determined. To check the specificity, the samples were subjected to spectrophotometric and chromatographic analysis. To assess specific activity, blood samples of 17 people immunized with the plague live vaccine from the Yersinia pestis EV strain of the NIIEG line were used for epidemic indications. As a comparative control, a sterile isotonic sodium chloride solution was used. The contingent was examined before vaccination on days 7, 21 and 3 months after immunization by evaluating the expression intensity of basophils CD63. Biochemical analysis of the obtained by the modified procedure of the pestin and derivatives allowed to judge the qualitative composition, to show the absence of impurities of the protein nature, as well as to determine the carbohydrate profile. The use of the drug as an allergen to assess the formation of antiplague immunity in the vaccinated contingent confirmed its specificity. The obtained data showed the possibility and prospect of using the Pestin PP as a test allergen for the establishment of the reaction of activation of basophils in vitro

Системная и регионарная экстракорпоральная иммунокоррекция при гнойных заболеваниях легких

Systemic and regional extracorporal immunocorrection was used in complex treatment of 36 patients with acute lung suppuration. The method consisted of blood perfusion operation — leukocytapheresis with splenoplasmoperfusion with following fiberscope importation of cryopreserved autologic leukocytes into the pulmonary destruction focuses. Lung-pleural suppuration with the tendency to chronization of the process and with the drainaged focus, the tendency to intrapulmonary infection generalization are the indication for this procedure. Blood perfusion operation may be performed after the onset of the catabolic phase of lung suppuration only.The results showed that blood perfusion operations and following infusion of the autoleukocytes into the infection focuses lead to optimization of both systemic and regional immune reaction and to the more quick focus destruction healing.Использовалась системная и регионарная эктракорпоральная иммунокоррекция в комплексном лечении 36 больных с острым легочным нагноением. Метод состоял из операции перфузии крови — лейкоцитафереза со спленоплазмоперфузией с последующим фиброскопическим введением замороженных аутологичных лейкоцитов в очаг легочной деструкции. Легочно-плевральные нагноения с тенденцией к хронизации процесса и с дренированным очагом, с тенденцией к генерализации интрапульмональной инфекции являются показанием для проведения процедуры. Операция перфузии крови может быть выполнена только после начала катаболической фазы легочного нагноения.Результаты показали, что операции перфузии крови и последующая инфузия аутолейкоцитов в инфекционные очаги ведет к оптимизации как системной, так и регионарной иммунной реакции и к более быстрому разрешению очага деструкции